Functional Analysis of a β-Ketoacyl-CoA Synthase Gene, MpFAE2, by Gene Silencing in the Liverwort Marchantia polymorpha L.(Biochemistry & Molecular Biology)

概要

論文の詳細を見る
We have isolated a β-ketoacyl CoA synthase (KCS) gene, MpFAE2, from a liverwort, Marchantia polymorpha, and identified its substrate specificity using the technique of dsRNA-mediated gene silencing and overexpression. KCS catalyzes an essential reaction in the fatty acid elongation process, i. e., condensation of malonyl-CoA with acyl-CoA. By introducing a construct with a hairpin structure containing a partial MpFAE2 gene, the level of the MpFAE2 gene expression was suppressed constitutively. The transgenic plants showed a specific accumulation of fatty acid 18 : 0. In contrast, in transgenic M. polymorpha plants overexpressing the MpFAE2 gene, fatty acid 22 : 0 is accumulated. These results indicate that the MpFAE2 gene product catalyzes the elongation steps of 18 : 0 to 20 : 0 and possibly also of 20 : 0 to 22 : 0.
社団法人日本農芸化学会の論文
2003-03-23