MpFAE3, aβ-Ketoacyl-CoA Synthase Gene in the Liverwort Marchantia polymorpha L., Is Preferentially Involved in Elongation of Palmitic Acid to Stearic Acid(Biochemistry & Molecular Biology)

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Fatty acid chain elongation is a crucial step in the biosynthesis of long chain fatty acids. An essential reaction in the elongation process is condensation of malonyl-CoA with acyl-CoA, which is catalyzed by β-ketoacyl-CoA synthase (KCS) in plants. We have isolated and characterized the MpFAE3 gene, one of the KCS gene family in the liverwort Marchantia polymorpha. Transgenic M. polymorpha plants overexpressing Mp-FAE3 accumulate fatty acids 18 : 0, 20 : 0, and 22 : 0. In these plants, the amount of 16 : 0 is reduced to 50% of wild type. In a heterologous assay, transgenic methylotrophic yeast expressing the MpFAE3 gene accumulates fatty acid 18 : 0 and generates several longer fatty acids which are not detectable in the control, accompanied by a decrease of 16 : 0. These observations indicate that the MpFAE3 protein is preferentially involved in the elongation of 16 : 0 to 18 : 0 and also in the subsequent steps of 18 : 0 to 20 : 0 and 20 : 0 to 22 : 0 in M. polymorpha.
社団法人日本農芸化学会の論文
2003-08-23