Pyruvate Decarboxylase Encoded by the PDC1 Gene Contributes, at Least Partially, to the Decarboxylation of α-Ketoisocaproate for Isoamyl Alcohol Formation in Saccharomyces cerevisiae

概要

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Isoamyl alcohol is an important flavor component of yeast-fermented alcoholic beverages. To identify the enzyme and gene involved in the decarboxylation of α-ketoisocaproate(α-KIC)for isoamyl alcohol formation, the enzyme was partially purified and analyzed by mass spectrometry. The pyruvate decarboxylase encoded by the PDC1 gene was considered a likely candidate enzyme. Genetic analysis showed that the activity of α-KIC decarboxylase and production of isoamyl alcohol partially decreased in a pdc1 null mutant and increased in a transformant with a multi-copy plasmid carrying the PDC1 gene. These results indicate that pyruvate decarboxylase encoded by the PDC1 gene contributes, at least partially, to the decarboxylation of α-KIC for isoamyl alcohol formation.
社団法人日本生物工学会の論文
2001-07-25