Cloning and Nucleotide Sequence of the Alcohol Acetyltransferase II Gene (ATF2) from Saccharomyces cerevisiae Kyokai No.7

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概要

• 論文の詳細を見る

• The ATF2 gene, which encodes alcohol acetyltransferase II (AATase II), was cloned from Saccharomyces cerevisiae Kyokai No.7 (sake yeast). The ATF2 gene coded for a protein of 535 amino acid residues with a calculated molecular mass of 61,909 daltons. The deduced amino acid sequences of the ATF2 showed 36.9% similarity with that of ATF1,which encodes AATase I. The hydrophobicity profiles for the Atf2 protein and Atf1 protein were similar. A transformant carrying multiple copies of the ATF2 gene had 2.5-fold greater AATase activity than the control, and this activity was not significantly inhibited by linoleic acid. A Southern analysis of the yeast genomes in which the ATF2 gene was used as a probe showed that S. cerevisiae and brewery lager yeast have one ATF2 gene, while S. bayanus has no similar gene.

• 社団法人日本農芸化学会の論文
• 1998-10-23

著者

• IWAMATSU Akihiro

  Central Lab. Key Tech., Kirin Brewery Co.

• Iwamatsu A

  Kirin Brewery Co. Ltd. Kanagawa Jpn

• Iwamatsu A

  Kirin Brewery Co. Ltd. Yokohama Jpn

• Iwamatsu Akihiro

  Central Lab. Key Tech. Kirin Brewery Co.

• NAGASAWA Naoshi

  General Research Laboratory, Ozeki Corp

• BOGAKI Takayuki

  General Research Laboratory, Ozeki Corp

• HAMACHI Masaaki

  General Research Laboratory, Ozeki Corp

• KUMAGAI Chieko

  General Research Laboratory, Ozeki Corp

• Kumagai Chieko

  General Research Laboratories Ozeki Corp.

• Kumagai C

  Ozeki Corp. Hyogo Jpn

• Bogaki Takayuki

  General Research Laboratory Ozeki Corp

• Bogaki Takayuki

  General Research Laboratory Ozeki Co. Ltd.

• Nagasawa Naoshi

  General Research Laboratory Ozeki Corp

• Hamachi M

  Ozeki Corp. Hyogo Jpn

• Hamachi Masaaki

  General Research Laboratory Ozeki Corp.

• IWAMATSU Akihiro

  Central Lab. Key Tech. , Kirin Brewery Co.

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