A Method for the Re-isolation of an Autonomously Replicating Plasmid from Aspergillus Transformants
スポンサーリンク
概要
- 論文の詳細を見る
An autonomously replicating plasmid is expected to increase the frequency of Aspergillus transformation. To construct this type of plasmid, we developed a rapid method of re-isolating autonomously replicating plasmids from Aspergillus transformants. Transformants grown in MM medium under selective pressure for 1-2 days were converted to protoplasts with a cell wall lytic enzyme (e. g. Yatalase). The protoplasts were lysed with phenol/chloroform followed by precipitation with ethanol. The total DNA was treated with RNaseA, re-precipitated with PEG, and then used to transform E. coli. These re-isolated plasmids were mainly the plasmid monomer.
- 社団法人日本農芸化学会の論文
- 1995-06-23
著者
-
Ozeki K
Ozeki Corp. Hyogo Jpn
-
Ozeki Kenji
General Research Laboratory Ozeki Corp.
-
Ozeki Kenji
General Research Laboratories Ozeki Corp.
-
HAMACHI Masaaki
General Research Laboratory, Ozeki Corp
-
Kanda Akihiro
Process And Systems Development Group Production Technology Center Kaneka Corporation
-
KANDA Akihiro
General Research Laboratory, Ozeki Corporation
-
NUNOKAWA Yataro
General Research Laboratory, Ozeki Corporation
-
Hizume Kazuhisa
General Research Laboratory, Ozeki Corporation
-
Hizume Kazuhiro
General Research Laboratory, Ozeki Corporation
-
Kanda Akihiro
General Research Laboratory Ozeki Corporation
-
Hizume Kazuhiro
General Research Laboratory Ozeki Corporation
-
Nunokawa Y
General Research Laboratory Ozeki Corporation
-
Hizume Kazuhisa
General Research Laboratory Ozeki Co.
-
Ohbuchi K
Ozeki Corp. Hyogo Jpn
-
Hamachi M
Ozeki Corp. Hyogo Jpn
-
Hamachi Masaaki
General Research Laboratory Ozeki Corp.
関連論文
- Cloning and Nucleotide Sequence of the Alcohol Acetyltransferase II Gene (ATF2) from Saccharomyces cerevisiae Kyokai No.7
- The Purification, Properties and Internal Peptide Sequences of Alcohol Acetyltransferase Isolated from Saccharomyces cerevisiae Kyokai No. 7
- A Specific Method for the Detection of Hiochi Bacteria Using an Enzyme-linked Immunosorbent Assay System with Anti-hiochi Bacteria Monoclonal Antibodies
- Identification of Hiochi Bacteria by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoretic Patterns of Whole Cell Proteins(Microbiology & Fermentation Industry)
- Optimizing Control of Sensory Evaluation in the Sake Mashing Process by Decentralized Learning of Fuzzy Inferences Using a Genetic Algorithm
- Ethyl α-D-Glucoside Increases Urine Volume and Causes Renal Morphologic Changes in Rats
- Construction of a Promoter Probe Vector Autonomously Maintained in Aspergillus and Characterization of Promoter Regions Derived from A. niger and A. oryzae Genomes
- Alcohol Dehydrogenase I of Sake Yeast Saccharomyces cerevisiae Kyokai No. 7
- A Method for the Re-isolation of an Autonomously Replicating Plasmid from Aspergillus Transformants
- Transformation of Intact Aspergillus niger by Electroporation
- Plasmids of Hiochi-Bacteria
- Molecular Cloning and Characterization of a Transcriptional Activator Gene, amyR, Involved in the Amylolytic Gene Expression in Aspergillus oryzae
- Isolation of a New Lytic Enzyme for Hiochi Bacteria and Other Lactic Acid Bacteria
- Acceleration of Cell Growth and Ester Formation by Ultrasonic Wave Irradiation
- Rapid and Sensitive Detection of Hiochi Bacteria by Amplification of Hiochi Bacterial Common Antigen Gene by PCR Method and Characterization of the Antigen
- Hepatoprotective Effects of a Concentrate and Components of Sake against Galactosamine (GalN)-Induced Liver Injury in Mice
- Modeling of the Sensory Evaluation of Sake by Dempster-Shafer's Measure and Genetic Algorithm
- Thermal Control Strategy for Isoamylacetate Formation in Sake Brewed with a Saccharified Rice Solution