The Purification, Properties and Internal Peptide Sequences of Alcohol Acetyltransferase Isolated from Saccharomyces cerevisiae Kyokai No. 7
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概要
- 論文の詳細を見る
Alcohol acetyltransferase (AATase) catalyzes the esterification of isoamyl alcohol by acetyl coenzyme A. The enzyme was solubilized from the microsomal fraction of Saccharomyces cerevisiae Kyokai No. 7, using Triton X-100 and then purified by a series of chromatographic separations: Poly Buffer Exchanger 94 (PBE94), DEAE Toyopearl, Toyopearl HW60, hydroxyapatite, Octyl-Sepharose CL-4B, and hexanolaffinity column chromatography. When the solubilized enzyme was put on PBE94, two active fractions were obtained. The enzyme obtained after affinity column chromatography had a single band on an SDS polyacrylamide gel, and its molecular mass was estimated to be 60 kDa. The enzyme was most active at pH 8.0 and 25℃. It was stable between pH 7.5 and 8.5, but was unstable at tempratures above 10℃. The activity was markedly inhibited by heavy metal ions such as Cd^<2+>, Cu^<2+>, Zn^<2+>, and Hg^<2+>, and sulfhydryl reagents. The K_m for acetyl-CoA was 0.19 mM. The internal peptide sequences were also identified.
- 社団法人日本農芸化学会の論文
- 1993-12-23
著者
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IWAMATSU Akihiro
Central Lab. Key Tech., Kirin Brewery Co.
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Iwamatsu A
Kirin Brewery Co. Ltd. Kanagawa Jpn
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Iwamatsu A
Kirin Brewery Co. Ltd. Yokohama Jpn
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Iwamatsu Akihiro
Central Lab. Key Tech. Kirin Brewery Co.
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BOGAKI Takayuki
General Research Laboratory, Ozeki Corp
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HAMACHI Masaaki
General Research Laboratory, Ozeki Corp
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Minetoki Toshitaka
General Research Laboratory, Ozeki Corp.
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Fujii Toshio
Central Laboratories for Key Technology, Kirin Brewery Co., Ltd.
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Minetoki T
General Research Laboratory Ozeki Co. Ltd.
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Minetoki Toshitaka
General Research Laboratory Ozeki Corp.
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Bogaki Takayuki
General Research Laboratory Ozeki Corp
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Bogaki Takayuki
General Research Laboratory Ozeki Co. Ltd.
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Hamachi M
Ozeki Corp. Hyogo Jpn
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Hamachi Masaaki
General Research Laboratory Ozeki Corp.
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Fujii Toshio
Central Laboratories For Key Technology Kirin Brewery Co. Ltd.
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Fujii Toshio
Central Lab.key Tech.kirin Brewery Co.
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IWAMATSU Akihiro
Central Lab. Key Tech. , Kirin Brewery Co.
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