Improvement of the Aspergillus oryzae Enolase Promoter (P-enoA) by the Introduction of cis-Element Repeats
スポンサーリンク
概要
- 論文の詳細を見る
We constructed a protein expression vector with an improved enoA promoter that harbored 12 tandem repeats of the cis-acting element (region III) of Aspergillus oryzae. The improved promoter yielded reporter β-glucuronidase (GUS) activity approximately 30-fold of the original promoter. Northern blot analysis confirmed that GUS expression was increased at the transcriptional level. The transformant harboring seven copies of the novel vector showed more than 100,000 U/mg GUS protein, which was approximately 30% of all the cell-free soluble proteins.
- 社団法人 日本農芸化学会の論文
- 2005-01-23
著者
-
Minetoki Toshitaka
General Research Laboratory, Ozeki Corp.
-
Machida Masayuki
National Institute Of Advanced Industrial Science And Technology (aist)
-
Machida Masayuki
National Inst. Of Bioscience And Human-technology
-
HIROTSUNE MASATO
General Research Laboratory, Ozeki Corp.
-
TSUBOI Hirokazu
General Research Laboratory, Ozeki Corporation
-
KODA Akio
General Research Laboratory, Ozeki Corporation
-
TODA Tomomi
National Institute of Advanced Industrial Science and Technology (AIST)
-
Minetoki T
General Research Laboratory Ozeki Co. Ltd.
-
Minetoki Toshitaka
General Research Laboratory Ozeki Corp.
-
Koda Akio
General Research Laboratory Ozeki Co. Ltd.
-
Tsuboi Hirokazu
General Research Laboratory Ozeki Corporation
-
Hirotsune Masato
General Research Laboratory Ozeki Co. Ltd.
-
Hirotsune Masato
General Research Laboratory Ozeki Co.
関連論文
- Characteristic Expression of Three Amylase-encoding Genes, agdA, amyB, and glaA in Aspergillus oryzae Transformants Containing Multiple Copies of the agdA Gene
- Nucleotide Sequence and Expression of α-GIucosidase-encoding Gene (agdA) from Aspergillus oryzae
- The Purification, Properties and Internal Peptide Sequences of Alcohol Acetyltransferase Isolated from Saccharomyces cerevisiae Kyokai No. 7
- Optimizing Control of Sensory Evaluation in the Sake Mashing Process by Decentralized Learning of Fuzzy Inferences Using a Genetic Algorithm
- Rapid Detection of Homologously Integrated DNA Fragments and Accurate Quantitation of Their Copy Number in Transgenic Aspergillus oryzae by PCR
- Molecular Cloning and Characterization of a Transcriptional Activator Gene, amyR, Involved in the Amylolytic Gene Expression in Aspergillus oryzae
- 3F0930 Unidirectional alignment of stretched DNA molecules on an atomically flat surface
- Improvement of the Aspergillus oryzae Enolase Promoter (P-enoA) by the Introduction of cis-Element Repeats
- High Expression of a Synthetic Gene Encoding Potato α-Glucan Phosphorylase in Aspergillus niger(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Development of high expression system with the improved promoter using the cis-acting element in Aspergillus species
- Acceleration of Cell Growth and Ester Formation by Ultrasonic Wave Irradiation
- Improvement of the Lectin-Antibody Enzyme Immunoassay of the Alphafetoprotein Carbohydrate Chain for Automation with the Enzyme Immunoassay Robot
- Analysis of the Pyruvate Permease Gene (JEN1) in Glucose Derepression Yeast (Saccharomyces cerevisiae) Isolated from a 2-Deoxyglucose-tolerant Mutant, and Its Application to Sake Making(Microbiology & Fermentation Technology)
- Hepatoprotective Effects of a Concentrate and Components of Sake against Galactosamine (GalN)-Induced Liver Injury in Mice
- Modeling of the Sensory Evaluation of Sake by Dempster-Shafer's Measure and Genetic Algorithm