Rapid Detection of Homologously Integrated DNA Fragments and Accurate Quantitation of Their Copy Number in Transgenic Aspergillus oryzae by PCR
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概要
- 論文の詳細を見る
A simple and rapid method for the analysis of artificially introduced DNA fragments has been developed using competitive PCR and long and accurate PCR. The locus and the copy number of the DNA fragments in each Aspergillus oryzae tranformant could be detected more rapidly and accurately by this method than by the conventional Southern hybridization method.
- 社団法人日本生物工学会の論文
- 2000-11-25
著者
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Minetoki Toshitaka
General Research Laboratory, Ozeki Corp.
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Machida Masayuki
Department Of Molecular Biology National Institute Of Bioscience And Human-technology
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Machida Masayuki
Department Of Molecular Biology National Institute Of Bioscience And Human Technology
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Gomi Katsuya
Faculty Of Agriculture Tohoku University
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Minetoki Toshitaka
General Research Laboratory Ozeki Corp.
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HONDA MUNECHIKA
Department of Plant Biotechnology, Gifu Prefectural Institute for Bioindustrial Technology
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Honda Munechika
Department Of Plant Biotechnology Gifu Prefectural Institute For Bioindustrial Technology
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MACHIDA Masayuki
Department of Agricultural Chemistry, The University of Tokyo
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