Application of Long-distance PCR to Restriction Site Mapping of a Cloned DNA Fragment on the λEMBL3 Phage Vector

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概要

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• Long-distance PCR was applied to rapidly map the restriction sites of long inserts cloned on λEMBL3 phage vector. The restriction sites of 9 of 15 enzymes were completely assigned in a model experiment within 14 h, including 8 h for the PCR amplification. This method was found particularly useful for genomic DNA cloning when the partial sequence of the corresponding cDNA is known.

• 社団法人日本農芸化学会の論文
• 1996-06-23

著者

• MANABE Mariko

  National Food Research Institute

• Machida Masayuki

  Department Of Molecular Biology National Institute Of Bioscience And Human-technology

• Machida Masayuki

  Department Of Molecular Biology National Institute Of Bioscience And Human Technology

• Jigami Yoshifumi

  Department Of Molecular Biology National Institute Of Bioscience And Human-technology

• YASUKAWA Makoto

  Fukushima Technology Center

• MACHIDA Masayuki

  Department of Agricultural Chemistry, The University of Tokyo

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