High Expression of a Synthetic Gene Encoding Potato α-Glucan Phosphorylase in Aspergillus niger(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
We describe the successful heterologous expression of the Solanum tuberosum α-glucan phosphorylase (GP) gene in Aspergillus niger. Special attention was paid to the influence of different codon usage and A+T content in the coding region on GP protein expression. Use of A. niger-preferred codon usage and lower A+T content in a synthetic gene (GP-syn) resulted in a significant improvement in the level of the GP mRNA and a dramatic increase in the quantity of GP protein produced such that it accounted for approximately 10% of the total soluble protein. We suggest that redesigning the primary DNA sequence encoding a desired protein product can be an extremely effective method for improving heterologous protein production in filamentous fungi.
- 社団法人日本生物工学会の論文
- 2005-11-25
著者
-
BOGAKI Takayuki
General Research Laboratory, Ozeki Corp
-
Minetoki Toshitaka
General Research Laboratory, Ozeki Corp.
-
HIROTSUNE MASATO
General Research Laboratory, Ozeki Corp.
-
KODA Akio
General Research Laboratory, Ozeki Corporation
-
Minetoki T
General Research Laboratory Ozeki Co. Ltd.
-
Minetoki Toshitaka
General Research Laboratory Ozeki Corp.
-
Koda Akio
General Research Laboratory Ozeki Co. Ltd.
-
Bogaki Takayuki
General Research Laboratory Ozeki Co. Ltd.
-
Hirotsune Masato
General Research Laboratory Ozeki Co. Ltd.
-
Hirotsune Masato
General Research Laboratory Ozeki Co.
関連論文
- Characteristic Expression of Three Amylase-encoding Genes, agdA, amyB, and glaA in Aspergillus oryzae Transformants Containing Multiple Copies of the agdA Gene
- Nucleotide Sequence and Expression of α-GIucosidase-encoding Gene (agdA) from Aspergillus oryzae
- Cloning and Nucleotide Sequence of the Alcohol Acetyltransferase II Gene (ATF2) from Saccharomyces cerevisiae Kyokai No.7
- The Purification, Properties and Internal Peptide Sequences of Alcohol Acetyltransferase Isolated from Saccharomyces cerevisiae Kyokai No. 7
- Optimizing Control of Sensory Evaluation in the Sake Mashing Process by Decentralized Learning of Fuzzy Inferences Using a Genetic Algorithm
- Rapid Detection of Homologously Integrated DNA Fragments and Accurate Quantitation of Their Copy Number in Transgenic Aspergillus oryzae by PCR
- Molecular Cloning and Characterization of a Transcriptional Activator Gene, amyR, Involved in the Amylolytic Gene Expression in Aspergillus oryzae
- Improvement of the Aspergillus oryzae Enolase Promoter (P-enoA) by the Introduction of cis-Element Repeats
- High Expression of a Synthetic Gene Encoding Potato α-Glucan Phosphorylase in Aspergillus niger(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Development of high expression system with the improved promoter using the cis-acting element in Aspergillus species
- Acceleration of Cell Growth and Ester Formation by Ultrasonic Wave Irradiation
- Analysis of the Pyruvate Permease Gene (JEN1) in Glucose Derepression Yeast (Saccharomyces cerevisiae) Isolated from a 2-Deoxyglucose-tolerant Mutant, and Its Application to Sake Making(Microbiology & Fermentation Technology)
- Hepatoprotective Effects of a Concentrate and Components of Sake against Galactosamine (GalN)-Induced Liver Injury in Mice
- Modeling of the Sensory Evaluation of Sake by Dempster-Shafer's Measure and Genetic Algorithm