Characteristic Expression of Three Amylase-encoding Genes, agdA, amyB, and glaA in Aspergillus oryzae Transformants Containing Multiple Copies of the agdA Gene
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概要
- 論文の詳細を見る
In Aspergillus oryzae wild-type strains, the expression of the agdA gene encoding α-glucosidase (AGL) is induced by maltose at the transcriptional level in a similar manner to the amyB gene encoding Takaamylase A (TAA) and the glaA gene encoding glucoamylase (GLA). In A. oryzae transformants containing multiple copies of the agdA gene, a high-level of AGL activity was observed. This was accompanied by a significant reduction in TAA and GLA activities. Moreover, transformants with the highest AGL activity showed the lowest degree of TAA and GLA activities. Northern blot analyses showed that the transcriptional levels of amyB and glaA in the AGL-overproducing transformant were drastically reduced when large amounts of agdA mRNA were detected in maltose-grown mycelia. In addition, the glucose concentration of the maltose-containing medium that was used to grow the AGL-overproducing transformant for RNA extraction was higher than that of the control transformant. These results suggest that the reduced expression of the amyB and glaA genes in the AGL-overproducing transformant was due to either titration of a common regulatory protein(s) involved in maltose induction or carbon catabolite repression.
- 社団法人日本農芸化学会の論文
- 1995-12-23
著者
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Kitamoto K
Univ. Tokyo Tokyo Jpn
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Kitamoto K
National Research Institute Of Brewing
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Tamura G
Department Of Agricultural Chemistry The University Of Tokyo
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Tamura G
Research Institute Of Brewing Resources Co. Ltd.
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Tamura Gakuzo
Research Institute Of Brewing Resources Co. Ltd.
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Kitamoto Katsuhiko
Department Of Biotechnology University Of Tokyo
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Kitamoto Katsuhiko
National Research Institute Of Brewing
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GOMI Katsuya
National Research Institute of Brewing
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Tamura Gakuzo
Nuclear Receptor Institute Ksp
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KUMAGAI Chieko
National Research Institute of Brewing
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Minetoki Toshitaka
Research Institute of Brewing Resources Co., Ltd.
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Kumagai C
National Research Institute Of Brewing
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Gomi K
Tohoku Univ. Miyagi Jpn
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Kumagai Chieko
General Research Laboratories Ozeki Corp.
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Minetoki T
General Research Laboratory Ozeki Co. Ltd.
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Kimura K
Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokkaido University
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