Isolation of a New Lytic Enzyme for Hiochi Bacteria and Other Lactic Acid Bacteria
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概要
- 論文の詳細を見る
A microorganism producing a lytic enzyme preparation that could rapidly lyse bacterial cells such as hiochi bacteria and other lactic acid bacteria was screened. The microorganism was identified as Streptomyces fulvissimus. The enzyme produced by this organism lysed boil-denatured cells quicker than intact cells of hiochi bacteria. A mutant strain of S.fulvissimus producing the enzyme exhibiting high activity against intact cells of hiochi bacteria was screened on plates, containing intact cells inactivated with UV irradiation. The optimal pH for lytic activity against intact cells of the hiochi bacterium Lactobacillus casei S-4 was from 3.5 to 4.0, and the optimum temperature was close to 50℃. This enzyme activity was stable between pH 3.5 and pH 8.0 and up to 60℃. The enzyme exhibits N-acetyl glucosaminidase and muramidase activities. The effects of adjusting the pH and using different inducers for enzyme production were investigated. Chitin was the most effective inducer of enzyme production. Intact DNA was easily isolated from the cells of many lactic acid bacteria following lysis with the enzyme. It is thought that this enzyme will be a good biotechnological tool.
- 社団法人日本生物工学会の論文
- 2001-05-25
著者
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Hasegawa Kazuya
General Research Laboratory Ozeki Corp.
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Ozeki Kenji
General Research Laboratory Ozeki Corp.
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Ozeki Kenji
General Research Laboratories Ozeki Corp.
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HAMACHI Masaaki
General Research Laboratory, Ozeki Corp
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Kumagai Chieko
General Research Laboratories Ozeki Corp.
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Kumagai Chieko
General Research Laboratory Ozeki Corp.
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OHBUCHI KAZUHIKO
General Research Laboratory, Ozeki Corporation
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Ohbuchi Kazuhiko
General Research Laboratory Ozeki Corp.
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Hamachi Masaaki
General Research Laboratory Ozeki Corp.
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