Transformation of the Industrial Strain of Aspergillus oryzae with the Homologous amdS Gene as a Dominant Selectable Marker

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概要

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• A homologous transformation system has been developed for Aspergillus oryzae industrial strains using the A. oryzae acetamidase-encoding gene (amdS) as a dominant selectable marker. Although the transformation efficiency is relatively low (0.4-1 transformants per μg DNA), the transformants contained significantly high copy numbers of the plasmid integrated into the genome. In addition, the promoter of the amdS gene was replaced by the strong expression promoter of the α-amylase-encoding gene (amyB) from A. oryzae, thereby enhancing the selection of the tranformants.

• 社団法人日本生物工学会の論文
• 1992-12-25

著者

• Kitamoto K

  Univ. Tokyo Tokyo Jpn

• Kitamoto K

  National Research Institute Of Brewing

• Kitamoto Katsuhiko

  Department Of Biotechnology University Of Tokyo

• GOMI Katsuya

  National Research Institute of Brewing

• Kumagai C

  National Research Institute Of Brewing

• Gomi K

  Tohoku Univ. Miyagi Jpn

• Kumagai Chieko

  General Research Laboratories Ozeki Corp.

• GOMI KATSUYA

  Natinal Research Institute of Brewing.

• KITAMOTO KATSUHIKO

  Natinal Research Institute of Brewing.

• KUMAGAI CHIEKO

  Natinal Research Institute of Brewing.

• Kimura K

  Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokkaido University

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