Cloning and Functional Analysis of the Aspergillus oryzae Conidiation Regulator Gene brlA by Its Disruption and Misscheduled Expression
スポンサーリンク
概要
- 論文の詳細を見る
We cloned the brlA gene from Aspergillus oryzae genomic DNA using the A.nidulans brlA gene as a probe. A 3.1-kb EcoRI-BalI genomic DNA fragment was cloned and sequenced. The deduced amino acid sequence revealed 70% identity with A.nidulans BRLA and contained two C2H2 zinc finger motifs in its carboxyl terminus, and the promoter sequence contained a 43-bp highly conserved region, indicating that the cloned gene was an A.oryzae homologue of A.nidulans brlA. Disruption of the brlA gene by homologous recombination resulted in the loss of ability to form conidiophores. These results suggest that the brlA gene of A.oryzae plays a fundamental role in controlling conidiophore development. When the brlA gene was expressed under the control of the amyB promoter in A.oryzae transformants, highly differentiated and compact colonies were observed on a solid medium. The misscheduled expression of the brlA gene in submerged culture, in which conidiation does not normally occur, caused the development of complex conidiophore structures with vesicles, phialides and conidia.
- 社団法人日本生物工学会の論文
- 1999-04-25
著者
-
Yamada Osamu
National Cardiovascular Center
-
Iimura Yuzuru
National Research Institute of Brewing
-
Lee B
National Research Institute Of Brewing
-
Ishida Yuumi
Nite Biological Resource Center (nbrc) National Institute Of Technology And Evaluation
-
Iimura Yuzuru
Division Of Applied Biological Sciences Interdisciplinary Graduate School Of Medicine And Engineerin
-
GOMI Katsuya
National Research Institute of Brewing
-
Lee B
Laboratory Of Molecular Biology And Microbial Chemistry Tokyo University Of Agriculture And Technolo
-
Lee Byung
National Research Institute Of Brewing
-
Gomi K
Tohoku Univ. Miyagi Jpn
-
Yamato Osamu
Kagoshima Univ. Kagoshima
-
Iimura Y
National Research Institute Of Brewing
-
Yamada O
Laboratory Of Clinical Pathology Department Of Veterinary Clinical Sciences Faculty Of Agriculture K
関連論文
- FLO11 Is the Primary Factor in Flor Formation Caused by Cell Surface Hydrophobicity in Wild-Type Flor Yeast
- Diversity Analysis of Actinomycetes Assemblages Isolated from Soils in Cool-temperate and Subtropical Areas of Japan
- Thyroid Microsomal Antibody Test Predicts Hyperthyroidism during Prostacyclin Therapy in Patients with Primary Pulmonary Hypertention
- Isolation and Characterization of a Yeast Cryptococcus sp. S-2 That Produces Raw Starch-digesting α-Amylase, Xylanase, and Polygalacturonase
- PJ-335 Clinical Features and Prognosis of Idiopathic Restrictive Cardiomyopathy in Children(Cardiomyopathy, basic/clinical-5 (M) PJ57,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
- Construction of Uracil and Tryptophan Auxotrophic Mutants from Sake Yeasts by Disruption of URA3 and TRP1 Genes(Microbiology & Fermentation Industry)
- Morphological Analysis of Corneal Opacity in Shiba Dog with GM1 Gangliosidosis(Anatomy)
- Effect of Yeast Fumarase Gene (FUM1) Dsruption on Production of Malic, Fumaric and Succinic Acids in Sake Mash
- Construction of Urea Non-producing Yeast Saccharomyces cerevisiae by Disruption of the CAR1 Gene(Microbiology & Fermentation Industry)
- Insertion Analysis of Putative Functional Elements in the Promoter Region of the Aspergillus oryzae Taka-amylase A Gene (amyB) Using a Heterologous Aspergillus nidulans amdS-lacZ Fusion Gene System
- Characteristic Expression of Three Amylase-encoding Genes, agdA, amyB, and glaA in Aspergillus oryzae Transformants Containing Multiple Copies of the agdA Gene
- Nucleotide Sequence and Expression of α-GIucosidase-encoding Gene (agdA) from Aspergillus oryzae
- Cloning and Nucleotide Sequence of the Calmodulin-Encoding Gene (cmdA) from Aspergillus oryzae
- High Level Secretion of Calf Chymosin Using a Glucoamylase-prochymosin Fusion Gene in Aspergillus oryzae
- Deletion Analysis of the Taka-amylase A Gene Promoter Using a Homologous Transformation System in Aspergillus oryzae
- Identification of the Promoter Region of the Taka-amylase A Gene Required for Starch Induction(Microbiology & Fermentation Industry)
- The Glucoamylase cDNA from Aspergillus oryzae : Its Cloning, Nucleotide Sequence, and Expression in Saccharomyces cerevisiae(Microbiology & Fermentation Industry)
- Expression of Integrin Subunits Depend on Bovine Endometrial Stromal Cells Cultured In Vitro
- -298- THE ACCURACY OF THE ESTIMATION OF PRESSURE GRADIENT BY DOPPLER ECHO IN THE NON-ORIFICE LIKE STENOSIS : Echo Cardiography : FREE COMMUNICATIONS(II) : PROCEEDINGS OF THE 51th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
- Development of an Integrative DNA Transformation System for the Yeast Hansenula anomala
- Molecular Cloning of the Glucoamylase Gene of Aspergillus shirousami and Its Expression in Aspergillus oryzae(Microbiology & Fermentation Industry)
- Cloning and Nucleotide Sequence of the Genomic Taka-amylase A Gene of Aspergillus oryzae(Microbiology & Fermentation Industry)
- Cloning and Nucleotide Sequence of the Ribonuclease T_1 Gene (rntA) from Aspergillus oryzae and Its Expression in Saccharomyces cerevisiae and Aspergillus oryzae
- Acid Xylanase from Yeast Cryptococcus sp. S-2: Purification, Characterization, Cloning, and Sequencing
- Molecular Cloning of CWP1: A Gene Encoding a Saccharomyces cerevisiae Cell Wall Protein Solubilized with Rarobacter faecitabidus Protease I
- Cloning and Expression of 1,2-α-Mannosidase Gene (fmanIB) from Filamentous Fungus Aspergillus oryzae : in Vivo Visualization of the FmanIBp-GFP Fusion Protein
- Cloning, Characterization and Overexpression of a Gene (pdiA) Encoding Protein Disulfide Isomerase of Aspergillus oryzae
- Virgisporangium aliadipatigenens sp. nov., Isolated from Soil in Iriomote Island and Emended Description of the Genus Virgisporangium
- FLO11 is essential for pellicle formation by wild pellicle-forming yeasts isolated from contaminated wines(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Productino of HM-1 Killer Toxin in Saccharomyces cerevisiae Transformed with the PDR4 Gene and δ-Sequence-Mediated Multi-Integration System
- Structural Relationships among Killer Toxins Secreted from the Killer Strains of the Genus Williopsis
- Isolation and Nucleotide Sequence of Hansenula anomala URA3 Gene Encoding Orotidine-5'-Phosphate Decarboxylase
- Molecular Cloning and Characterization of a Transcriptional Activator Gene, amyR, Involved in the Amylolytic Gene Expression in Aspergillus oryzae
- Molecular Cloning of a Genomic DNA for Enolase from Aspergillus oryzae
- A New Enrichment Method for the Selective Isolation of Streptomycetes from the Root Surfaces of Herbaceous Plants
- Stimulatory effect of indole-3-acetic acid on aerial mycelium formation and antibiotic production in Streptomyces spp.
- Two Alleles of the Sulfite Resistance Genes Are Differentially Regulated in Saccharomyces cerevisiae
- Distribution of the Sulfite Resistance Gene SSU1-R and the Variation in Its Promoter Region in Wine Yeasts(BREWING AND FOOD TECHNOLOGY)
- Species Diversity of Nocardiae Isolated from Lake and Moat Sediment Samples
- Cloning and Functional Analysis of the Aspergillus oryzae Conidiation Regulator Gene brlA by Its Disruption and Misscheduled Expression
- 904 Isolation and Characterization of Mutants of Saccharomyces cerevisiae with pH-sensitive Growth
- Electrophoretic Karyotype and Gene Assignment to Chromosomes of Aspergillus oryzae
- A Novel Culture Method for High Level Production of Heterologous Protein in Saccharomyces cerevisiae
- Cloning and Nucleotide Sequence of the Acid Protease-encoding Gene (pepA) from Aspergillus oryzae
- Mutant Isolation of Non-Urea Producing Sake Yeast by Positive Selection
- Transformation of the Industrial Strain of Aspergillus oryzae with the Homologous amdS Gene as a Dominant Selectable Marker
- Site-directed Mutagenesis of Catalytic Active-site Residues of Taka-amylase A
- Cloning and Heterologous Expression of the Antibiotic Peptide (ABP) Genes from Rhizopus oligosporus NBRC 8631
- CONTRACTILITY AND OXYGEN CONSUMFTION OF CANINE LEFT VENTRICLE : SYSTOLIC PRESSURE VOLUME AREA (PVA) : Cardiac Function (III) : III : 48 Annual Scientific Meeting, Japanese Circulation Society
- QUANTITATIVE ANALYSIS OF LEFT VENTRICULAR REGIONAL WORK BY WALL TENSION-AREA RELATION : Cardiac Function(II) : II : 48 Annual Scientific Meeting, Japanese Circulation Society
- The Promoter Activity of Isovaleryl-CoA Dehydrogenase-Encoding Gene (ivdA) from Aspergillus oryzae Is Strictly Repressed by Glutamic Acid
- The Breeding of Cryophilic Killer Wine Yeasts
- Loss of Aspergillus oryzae amyR function indirectly affects hemicellulolytic and cellulolytic enzyme production(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Aflatoxin non-productivity of Aspergillus oryzae caused by loss of function in the aflJ gene product(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- dffA Gene from Aspergillus oryzae Encodes L-Ornithine N^5-Oxygenase and Is Indispensable for Deferriferrichrysin Biosynthesis(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Gene Silencing by RNA Interference in the Koji Mold Aspergillus oryzae
- Analysis of the Pyruvate Permease Gene (JEN1) in Glucose Derepression Yeast (Saccharomyces cerevisiae) Isolated from a 2-Deoxyglucose-tolerant Mutant, and Its Application to Sake Making(Microbiology & Fermentation Technology)
- Production of Fungal Cell Wall Lytic Enzymes by Oerskovia sp. CK and Some Propertics of the Crude Enzymes.
- Transformation System for Aspergillus oryzae with Double Auxotrophic Mutations, niaD and sC
- Increase in Activity for the C-Terminal Pro-X Bond by Site-directed Mutagenesis of Gly137 to Ala in Carboxypeptidase Z
- Transformation of Aspergillus oryzae through Plasmid-mediated Complementation of the Methionine-auxotrophic Mutation(Microbiology & Fermentation Industry)
- Molecular biological researches of Kuro-Koji molds, their classification and safety(BREWING AND FOOD TECHNOLOGY)
- Development of an efficient gene-targeting system in Aspergillus luchuensis by deletion of the non-homologous end joining system(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Lack of endoplasmic reticulum 1,2-α-mannosidase activity that trims N-glycan Man_9GlcNAc_2 to Man_8GlcNAc_2 isomer B in a manE gene disruptant of Aspergillus oryzae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- L-Canavanine Resistance as a Positive Selectable Marker in Diploid Yeast Transformation through Integral Disruption of the CAN1 Gene
- Actinomycetospora iriomotensis sp. nov., a novel actinomycete isolated from a lichen sample
- Lack of endoplasmic reticulum 1,2-αmannosidase activity that trims N-glycan Man_9GlcNAc_2 to Man_8GlcNAc_2 isomer B in a manE gene disruptant of Aspergillus oryzae
- Cathodoluminescence of Terbium Activated Metaphosphate Glasses
- Isolation and Characterization of Starch-utilizing Mutants of Escherichia coli
- Yeast Transformation without the Spheroplasting Process
- Fatty Acids Associated with the Cell Wall in Film Strains of Saccharomyces