Transformation System for Aspergillus oryzae with Double Auxotrophic Mutations, niaD and sC
スポンサーリンク
概要
- 論文の詳細を見る
We developed a transformation system for Aspergillus oryzae using the Aspergillus nidulans sC gene encoding ATP sulfurylase as a selectable marker. The sC^- mutants can be readily isolated by positive selection for selenate resistance, thereby the niaD^- mutant strain of A. oryzae was bestowed with the sC^- mutation. Transformation of the A. oryzae host (niaD^-, sC^-) with the plasmid carrying A. nidulans sC gave random and multi-copy integrants, while that with the A. oryzae niaD-carrying plasmid occurred mainly by single-copy and homologous integration events (more than 50% frequency), indicating that with this transformation system, the transformation marker could be selected according to the integration pattern one desires.
- 社団法人日本農芸化学会の論文
- 1997-08-23
著者
-
Yamada Osamu
National Cardiovascular Center
-
Lee B
National Research Institute Of Brewing
-
GOMI Katsuya
National Research Institute of Brewing
-
Lee B
Laboratory Of Molecular Biology And Microbial Chemistry Tokyo University Of Agriculture And Technolo
-
Lee Byung
National Research Institute Of Brewing
-
Gomi K
Tohoku Univ. Miyagi Jpn
-
Yamato Osamu
Kagoshima Univ. Kagoshima
-
Yamada O
Laboratory Of Clinical Pathology Department Of Veterinary Clinical Sciences Faculty Of Agriculture K
関連論文
- Thyroid Microsomal Antibody Test Predicts Hyperthyroidism during Prostacyclin Therapy in Patients with Primary Pulmonary Hypertention
- PJ-335 Clinical Features and Prognosis of Idiopathic Restrictive Cardiomyopathy in Children(Cardiomyopathy, basic/clinical-5 (M) PJ57,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
- Construction of Uracil and Tryptophan Auxotrophic Mutants from Sake Yeasts by Disruption of URA3 and TRP1 Genes(Microbiology & Fermentation Industry)
- Morphological Analysis of Corneal Opacity in Shiba Dog with GM1 Gangliosidosis(Anatomy)
- Construction of Urea Non-producing Yeast Saccharomyces cerevisiae by Disruption of the CAR1 Gene(Microbiology & Fermentation Industry)
- Insertion Analysis of Putative Functional Elements in the Promoter Region of the Aspergillus oryzae Taka-amylase A Gene (amyB) Using a Heterologous Aspergillus nidulans amdS-lacZ Fusion Gene System
- Characteristic Expression of Three Amylase-encoding Genes, agdA, amyB, and glaA in Aspergillus oryzae Transformants Containing Multiple Copies of the agdA Gene
- Nucleotide Sequence and Expression of α-GIucosidase-encoding Gene (agdA) from Aspergillus oryzae
- Cloning and Nucleotide Sequence of the Calmodulin-Encoding Gene (cmdA) from Aspergillus oryzae
- High Level Secretion of Calf Chymosin Using a Glucoamylase-prochymosin Fusion Gene in Aspergillus oryzae
- Deletion Analysis of the Taka-amylase A Gene Promoter Using a Homologous Transformation System in Aspergillus oryzae
- Identification of the Promoter Region of the Taka-amylase A Gene Required for Starch Induction(Microbiology & Fermentation Industry)
- The Glucoamylase cDNA from Aspergillus oryzae : Its Cloning, Nucleotide Sequence, and Expression in Saccharomyces cerevisiae(Microbiology & Fermentation Industry)
- Expression of Integrin Subunits Depend on Bovine Endometrial Stromal Cells Cultured In Vitro
- -298- THE ACCURACY OF THE ESTIMATION OF PRESSURE GRADIENT BY DOPPLER ECHO IN THE NON-ORIFICE LIKE STENOSIS : Echo Cardiography : FREE COMMUNICATIONS(II) : PROCEEDINGS OF THE 51th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
- Molecular Cloning of the Glucoamylase Gene of Aspergillus shirousami and Its Expression in Aspergillus oryzae(Microbiology & Fermentation Industry)
- Cloning and Nucleotide Sequence of the Genomic Taka-amylase A Gene of Aspergillus oryzae(Microbiology & Fermentation Industry)
- Cloning and Nucleotide Sequence of the Ribonuclease T_1 Gene (rntA) from Aspergillus oryzae and Its Expression in Saccharomyces cerevisiae and Aspergillus oryzae
- Cloning and Expression of 1,2-α-Mannosidase Gene (fmanIB) from Filamentous Fungus Aspergillus oryzae : in Vivo Visualization of the FmanIBp-GFP Fusion Protein
- Cloning, Characterization and Overexpression of a Gene (pdiA) Encoding Protein Disulfide Isomerase of Aspergillus oryzae
- Molecular Cloning and Characterization of a Transcriptional Activator Gene, amyR, Involved in the Amylolytic Gene Expression in Aspergillus oryzae
- Molecular Cloning of a Genomic DNA for Enolase from Aspergillus oryzae
- Cloning and Functional Analysis of the Aspergillus oryzae Conidiation Regulator Gene brlA by Its Disruption and Misscheduled Expression
- 904 Isolation and Characterization of Mutants of Saccharomyces cerevisiae with pH-sensitive Growth
- Electrophoretic Karyotype and Gene Assignment to Chromosomes of Aspergillus oryzae
- A Novel Culture Method for High Level Production of Heterologous Protein in Saccharomyces cerevisiae
- Cloning and Nucleotide Sequence of the Acid Protease-encoding Gene (pepA) from Aspergillus oryzae
- Mutant Isolation of Non-Urea Producing Sake Yeast by Positive Selection
- Transformation of the Industrial Strain of Aspergillus oryzae with the Homologous amdS Gene as a Dominant Selectable Marker
- Site-directed Mutagenesis of Catalytic Active-site Residues of Taka-amylase A
- Cloning and Heterologous Expression of the Antibiotic Peptide (ABP) Genes from Rhizopus oligosporus NBRC 8631
- CONTRACTILITY AND OXYGEN CONSUMFTION OF CANINE LEFT VENTRICLE : SYSTOLIC PRESSURE VOLUME AREA (PVA) : Cardiac Function (III) : III : 48 Annual Scientific Meeting, Japanese Circulation Society
- QUANTITATIVE ANALYSIS OF LEFT VENTRICULAR REGIONAL WORK BY WALL TENSION-AREA RELATION : Cardiac Function(II) : II : 48 Annual Scientific Meeting, Japanese Circulation Society
- The Promoter Activity of Isovaleryl-CoA Dehydrogenase-Encoding Gene (ivdA) from Aspergillus oryzae Is Strictly Repressed by Glutamic Acid
- Loss of Aspergillus oryzae amyR function indirectly affects hemicellulolytic and cellulolytic enzyme production(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Aflatoxin non-productivity of Aspergillus oryzae caused by loss of function in the aflJ gene product(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- dffA Gene from Aspergillus oryzae Encodes L-Ornithine N^5-Oxygenase and Is Indispensable for Deferriferrichrysin Biosynthesis(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Gene Silencing by RNA Interference in the Koji Mold Aspergillus oryzae
- Analysis of the Pyruvate Permease Gene (JEN1) in Glucose Derepression Yeast (Saccharomyces cerevisiae) Isolated from a 2-Deoxyglucose-tolerant Mutant, and Its Application to Sake Making(Microbiology & Fermentation Technology)
- Production of Fungal Cell Wall Lytic Enzymes by Oerskovia sp. CK and Some Propertics of the Crude Enzymes.
- Transformation System for Aspergillus oryzae with Double Auxotrophic Mutations, niaD and sC
- Increase in Activity for the C-Terminal Pro-X Bond by Site-directed Mutagenesis of Gly137 to Ala in Carboxypeptidase Z
- Transformation of Aspergillus oryzae through Plasmid-mediated Complementation of the Methionine-auxotrophic Mutation(Microbiology & Fermentation Industry)
- Molecular biological researches of Kuro-Koji molds, their classification and safety(BREWING AND FOOD TECHNOLOGY)
- Development of an efficient gene-targeting system in Aspergillus luchuensis by deletion of the non-homologous end joining system(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Lack of endoplasmic reticulum 1,2-α-mannosidase activity that trims N-glycan Man_9GlcNAc_2 to Man_8GlcNAc_2 isomer B in a manE gene disruptant of Aspergillus oryzae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- L-Canavanine Resistance as a Positive Selectable Marker in Diploid Yeast Transformation through Integral Disruption of the CAN1 Gene
- Lack of endoplasmic reticulum 1,2-αmannosidase activity that trims N-glycan Man_9GlcNAc_2 to Man_8GlcNAc_2 isomer B in a manE gene disruptant of Aspergillus oryzae
- Cathodoluminescence of Terbium Activated Metaphosphate Glasses