Molecular Cloning of the Glucoamylase Gene of Aspergillus shirousami and Its Expression in Aspergillus oryzae(Microbiology & Fermentation Industry)
スポンサーリンク
概要
- 論文の詳細を見る
The glucoamylase enzyme (GAase) gene from Aspergillus shirousami was cloned and sequenced from genomic and cDNA libraries. The genomic gene was located in the 5.4 kb EcoRI fragment. The deduced amino acid sequence of GAase contained 639 amino acid residues with a relative molecular mass of approximately 68,000 daltons (non-glycosylated form). The genomic gene of A. shirousami GAase was introduced into Aspergillus oryzae. These transformants had increased GAase and raw starch degradation (RSD) activity in culture media and in rice-koji extracts. Analysis by Southern, Northern, SDS-PAGE, and Western blot techniques confirmed the foreign gene was correctly transcribed, translated, and expressed in A. oryzae.
- 社団法人日本農芸化学会の論文
- 1990-08-23
著者
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Iimura Yuzuru
National Research Institute of Brewing
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Tamura G
Department Of Agricultural Chemistry The University Of Tokyo
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Tamura Gakuzo
Research Institute Of Brewing Resources Co. Ltd.
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Shibuya I
Nikka Distilling Co. Ltd. Chiba Jpn
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Iimura Y
National Research Institute Of Brewing:(present Address) Department Of Applied Biochemistry Yamanash
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GOMI Katsuya
National Research Institute of Brewing
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TAKAHASHI Kojiro
National Research Institute of Brewing
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HARA Shodo
National Research Institute of Brewing
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Tamura Gakuzo
Nuclear Receptor Institute Ksp
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Tsuchiya Kozo
Research Institute of Brewing Resources Co.
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SHIBUYA Ichiro
Research Institute of Brewing Resources Co., Ltd.
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Takahashi K
Life Science Research Laboratory Japan Tobacco Inc.
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Hara S
General Research Laboratory Of Kiku-masamune Sake Brewing Co. Ltd.
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Hara Shodo
Office Of Technical Officers Osaka Regional Taxation Bureau
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Gomi K
Tohoku Univ. Miyagi Jpn
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Tsuchiya K
Nisshin Oil Mills Ltd. Yokohama‐shi Jpn
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