Productino of HM-1 Killer Toxin in Saccharomyces cerevisiae Transformed with the PDR4 Gene and δ-Sequence-Mediated Multi-Integration System
スポンサーリンク
概要
- 論文の詳細を見る
The gene for the HM-1 killer toxin of Williopsis mrakii IFO 0895,which has a broad killer spectrum and is stable over side pH and temperature ranges, was introduced into a killer-resistant mutant of Saccharomyces cerevisiae using a 2μm plasmid or δ-sequence-mediated multi-integration system under the control of the ADH1 promoter together with the PDR4 gene as a dominant selective marker. The killer activity of the transformants harboring the killer gene on a YEp plasmid was mitotically unstable and the plasmid copy number was reduced on a non-selective medium. In contrast, some transformants obtained using the δ-integration system showed strong killer activity which was mitotically stable even on a non-selective medium. Integration of multiple copies of the killer gene was confirmed by genomic Southern blot analysis. In a mixed culture experiment of the transformant using δ-mediated multi-integration which produces HM-1 and a HM-1-sensitive strain, Pichia anomala IFO 0569,the transformant prevented the growth of the latter.
- 社団法人日本生物工学会の論文
- 1995-11-25
著者
-
Kojima Yuzo
Department Of Bioscience Faculty Of Bioresources Center For Molecular Biology And Genetics Mie Unive
-
Kajino T
Special Research Laboratory Ii Toyota Central R&d Laboratories Inc.
-
Iimura Yuzuru
National Research Institute of Brewing
-
Ishida Yuumi
Nite Biological Resource Center (nbrc) National Institute Of Technology And Evaluation
-
Iimura Yuzuru
Division Of Applied Biological Sciences Interdisciplinary Graduate School Of Medicine And Engineerin
-
Kimura Tetsuya
National Center For Seeds And Seedlings
-
Kitamoto Noriyuki
Food Research Center, Aichi Industrial Technology Institute
-
Kojima Y
Medical Enzyme Division Amano Enzyme Co. Ltd.
-
Iimura Y
National Research Institute Of Brewing
-
KITO YUKIO
Food Research Institute, Aichi Prefectural Government
-
KIMURA TETSUYA
Food Research Institute, Aichi Prefectural Goverment
-
Kitamoto N
Food Research Center Aichi Industrial Technology Institute
-
Kitamoto Noriyuki
Food Research Center Aichi Industrial Technology Institute
-
Kito Yukio
Food Research Institute Aichi Prefectural Government
-
Kitamoto Noriyuki
Food Res. Center Aichi Ind. Tech. Inst.
関連論文
- Development of SSR Markers in Carnation (Dianthus caryophyllus)
- Characterization of Paenibacillus curdlanolyticus Intracellular Xylanase Xyn10B Encoded by the xyn10B Gene
- Molecular Cloning, Characterization, and Expression Analysis of the xynF3 Gene from Aspergillus oryzae(Microbiology & Fermentation Technology)
- Molecular Cloning, Overexpression, and Purification of a Major Xylanase from Aspergillus oryzae
- Analysis of the Promoter Activity of the Taka-Amylase Gene and the Phosphoglycerate Kinase Gene in a Shoyu-koji Mold Aspergillus oryzae KBN616
- Breeding of Yukirasha : Common Scab-Resistant Potato Variety for Table Stock
- Identification of a Catalytic Residue of Clostridium paraputrificum N-Acetyl-β-D-glucosaminidase Nag3A by Site-Directed Mutagenesis
- A Novel Thermophilic Pectate Lyase Containing Two Catalytic Modules of Clostridium stercorarium
- Detection of Hydrogen Gas-Producing Anaerobes in Refuse-Derived Fuel (RDF) Pellets
- Promoter of Arabidopsis thaliana Phosphate Transporter Gene Drives Root-Specific Expression of Transgene in Rice(Plant Biotechnology)
- Functions of Family-22 Carbohydrate-Binding Module in Clostridium thermocellum Xyn10C
- Cloning of the Ruminococcus albus cel5D and cel9A Genes Encoding Dockerin Module-containing Endoglucanases and Expression of cel5D in Escherichia coli
- Interaction between a Type-II Dockerin Domain and a Type-II Cohesin Domain from Clostridium thermocellum Cellulosome
- Analysis of Estrogen-Like Compounds in the Environment by High Performance Liquid Choromatography Bioassay (ENVIRONMENTAL BIOTECHNOLOGY)
- Sequencing and Expression of the Gene Encoding the Clostridium stercorarium β-Xylosidase Xyl43B in Escherichia coli
- Hydrogen Gas Generation from Refuse-derived Fuel (RDF) under Wet Conditions
- Cloning, Sequencing, and Expression of the Gene Encoding the Clostridium stercorarium α-Galactosidase Aga36A in Escherichia coli(Microbiology & Fermentation Technology)
- Electrotransformation of Clostridium paraputrificum M-21 with Some Plasmids(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- A New Type of β-N-Acetylglucosaminidase from Hydrogen-Producing Clostridium paraputrificum M-21(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- 3D11-3 In vitro growth and hydrolysis of cellulose and xylan by Emericella nidulans Rs1 in the presence of fermentation inhibitors
- Application of Microbial Genes to Recalcitrant Biomass Utilization and Environmental Conservation
- Stable Expression of a Thermostable Xylanase of Clostridium thermocellum in Cultured Tobacco Cells
- Isothermal Titration Calorimetric Studies on the Binding of a Family 6 Carbohydrate-binding Module of Clostridium thermocellum XynA with Xlylooligosaccharides(Biochemistry & Molecular Biology)
- 613 Cloning, Sequencing and Expression of The Gene Encoding the Clostridium stercorarium Pectate Lyase Pel9A in Escherichia coli
- Characterization of Clostridium paraputrificum Chitinase A from a Recombinant Escherichia coli
- Cloning, DNA Sequencing, and Expression of the β1,4-Mannanase Gene from a Marine Bacterium, Vibrio sp. Strain MA-138
- Sequence of the Clostridium thermocellum Mannanase Gene man26B and Characterization of the Translated Product
- Cloning and Sequencing of a Novel Endo-1,4-β-Glucanase Gene from Ruminococcus albus
- Effect of Pressure on the Association States of Enzyme-treated Caseins
- A Beta-Glucosidase Gene Downstream of the Cellulose Synthase Operon in Cellulose-producing Acetobacter
- Characterization of Bacillus halodurans α-Galactosidase Mel4A Encoded by the mel4A Gene (BH2228)
- Isolation and expression analysis of phosphate transporter genes from Eucalyptus camaldulensis
- DNA Profiling of Fresh and Processed Fruits in Pear
- Identification of Quince Varieties Using SSR Markers Developed from Pear and Apple
- Identification of Asian Pear Varieties by SSR Analysis
- Nucleotide Sequence of the Clostridium stercorarium xylA Gene Encoding a Bifunctional Protein with β-D-Xylosidase and α-L-Arabinofuranosidase Activities, and Properties of the Translated Product
- Cloning and Expression in Escherichia coli of Clostridium stercorarium Strain F-9 Genes Related to Xylan Hydrolysis(Microbiology & Fermentation Industry)
- Molecular Cloning of Xylanase Gene xynG1 from Aspergillus oryzae KBN 616,a Shoyu Koji Mold,and Analysis of Its Expression
- Estimation of Ploidy Levels and Breeding Backgrounds in Pot Carnation Cultivars Using Flow Cytometry and SSR Markers
- Genetic linkage map of the Japanese pear 'Housui' identifying three homozygous genomic regions
- Identification of Parent-offspring Relationships in 55 Japanese Pear Cultivars Using S-RNase Allele and SSR markers
- Integrated Reference Genetic Linkage Maps of Pear Based on SSR and AFLP Markers
- Physiological Responses to Maximal Treadmill and Deep Water Running in the Young and the Middle Aged Males
- Maximal Physiological Responses to Deep Water Running at Thermoneutral Temperature
- Purification and Characterization of the Family J Catalytic Domain Derived from the Clostridium thermocellum Endoglucanase CelJ
- Specific Adsorption of Clostridium stercorarium Xylanase to Amorphous Cellulose and Its Desorption by Cellobiose
- Cloning and Sequencing of the Gene Encoding Chitinase ChiA from Xanthomonas sp.Strain AK and Some Properties of ChiA
- Metalloregulatory Properties of the ArsR and ArsD Repressors of Acidiphilium multivorum AIU 301
- Stable Production of Thermotolerant Xylanase B of Clostridium stercorarium in Transgenic Tobacco and Rice
- Two Proteins with Diaphorase Activity from Clostridium thermocellum and Moorella thermoacetica
- Cloning and Expression of a Clostridium kluyveri Gene Responsible for Diaphorase Activity
- Characterization of a Dihydrolipoyl Dehydrogenase Having Diaphorase Activity of Clostridium kluyveri
- Detection of Anaerobic Ammonium-Oxidizing Bacteria in Ago Bay Sediments
- Tri-/Hexanucleotide Microsatellite Markers in Peach Derived from Enriched Genomic Libraries and Their Application in Rosaceae
- Effect of Family 22 Carbohydrate-Binding Module on the Thermostability of Xyn10B Catalytic Module from Clostridium stercorarium
- Comparison of Microbial Consortia in Refuse-Derived Fuel (RDF) Preparations between Japan and Germany
- 1G14-5 Properties of Bacillus haloduarans Xyn11A Expressed in Kluyveromyces lactis
- Different Binding Specificities of S-Layer Homology Modules from Clostridium thermocellum AncA, Slp1, and Slp2
- Enzymatic Properties of Two Catalytic Modules of Clostridium stercorarium Pectate Lyase Pel9A
- Purification and Characterization of the Lipase from Pseudomonas fluorescens HU380(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Increased Cellulose Production from Sucrose with Reduced Levan Accumulation by an Acetobacter Strain Harboring a Recombinant Plasmid
- Molecular Cloning of Xylanase Gene xynG1 from Aspergillus oryzae KBN 616, a Shoyu Koji Mold, and Analysis of Its Expression
- The Characterization of Acetic Acid Bacteria Efficiently Producing Bacterial Cellulose from Sucrose : The Proposal of Acetobacter xylinum subsp. nonacetoxidans subsp. Nov.
- High Rate Production in Static Culture of Bacterial Cellulose from Sucrose by a Newly Isolated Acetobacter Strain
- Screening of Bacterial Cellulose-producing Acetobacter Strains Suitable for Sucrose as a Carbon Source
- Productino of HM-1 Killer Toxin in Saccharomyces cerevisiae Transformed with the PDR4 Gene and δ-Sequence-Mediated Multi-Integration System
- The Nitrate Reductase Gene from a Shoyu Koji Mold, Aspergillus oryzae KBN616
- Structural Relationships among Killer Toxins Secreted from the Killer Strains of the Genus Williopsis
- Purification and Characterization of Xylanase A from CIostridium stercorarium F-9 and a Recombinant Escherichia coli
- Nucleotide Sequence of the Clostridium stercorarium xynA Gene Encoding Xylanase A : Identification of Catalytic and Cellulose Binding Domains
- Cloning and Sequencing of the Gene Encoding Cyclodextrin Glucanotransferase from Bacillus sp. KC201
- Purification, Characterization, and Molecular Cloning of Acidophilic Xylanase from Penicillium sp.40
- Characterization of the Cellulolytic Complex (Cellulosome) from Ruminococcus albus
- Sequence of egV and Properties of EgV, a Ruminococcus albus Endoglucanase Containing a Dockerin Domain
- Expression of a Bacterial Endoglucanase Gene in Tobacco Increases Digestibility of Its Cell Wall Fibers
- Adsorption of Clostridium stercorarium Xylanase A to Insoluble Xylan and the Importance of the CBDs to Xylan Hydrolysis
- High Expression of the Xylanase B Gene from Clostridium stercorarium in Tobacco Cells
- 478 Affinity of cellulose-binding domains of Clostridium stercorarium xylanase A for xylan and soluble saccharides and effect of connection of CBD to an endoglucanase on its enzyme activity
- Functions of Family-22 Carbohydrate-Binding Modules in Clostridium josui Xyn10A
- 3C11-1 Functions of family-22 carbohydrate-binding module in Clostridium josui Xyn10A
- Analysis of a Clostridium josui Cellulase Gene Cluster Containing the man5A Gene and Characterization of Recombinant Man5A
- Importance of the Carbohydrate-Binding Module of Clostridium stercorarium Xyn10B to Xylan Hydrolysis
- Characterization of a Dihydrolipoyl Dehydrogenase Having Diaphorase Activity of Clostridium kluyveri
- Two Proteins with Diaphorase Activity from Clostridium thermocellum and Moorella thermoacetica
- Cloning and Expression of a Clostridium kluyveri Gene Responsible for Diaphorase Activity
- Characterization of Paenibacillus curdlanolyticus Intracellular Xylanase Xyn10B Encoded by the xyn10B Gene
- Stable Production of Thermotolerant Xylanase B of Clostridium stercorarium in Transgenic Tobacco and Rice
- Stable Expression of the Chlorocatechol Dioxygenase Gene from Ralstonia eutropha NH9 in Hybrid Poplar Cells
- Characterization of Bacillus halodurans α-Galactosidase Me14A Encoded by the mel4A Gene (BH2228)
- Detection of Anaerobic Ammonium-Oxidizing Bacteria in Ago Bay Sediments
- Thermal Unfolding and Modular Architecture of Clostridium stercorarium Xyn10B
- High-Level Heterologous Expression of Bacillus halodurans Putative Xylanase Xyn11A (BH0899) in Kluyveromyces lactis
- Expression of Hydrogenase Gene in Clostridium Paraputrificum for Enhancing Hydrogen Gas Production (Proceedings of the Asia-Pacific Conference on Sustainable Energy and Environmental Technologies)
- Conversion of Chitinous Wastes to Hydrogen Gas by Clostridium paraputrificum M-21
- Identification and Characterization of Clostridium paraputrificum M-21, a Chitinolytic, Mesophilic and Hydrogen-Producing Bacterium
- Cloning, Sequencing, and Expression of the Gene Encoding the Clostridium stercorarium Xylanase C in Escherichia coli
- Expression of a Ruminococcus albus Cellulase Gene in Tobacco Suspension Cells
- Purification and Some Properties of a Chitinase from Xanthomonas sp. Strain AK
- Cloning, Sequencing, and Expression of the Gene Encoding a Cell-bound Multi-domain Xylanase from Clostridium josui, and Characterization of the Translated Product
- Molecular Breeding of Cellulolytic Microbes, Plants, and Animals for Biomass Utilization