Cloning and Expression in Escherichia coli of Clostridium stercorarium Strain F-9 Genes Related to Xylan Hydrolysis(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
Nine distinct fragments of Clostridium stercorarium strain F-9 (which was isolated and identified in this laboratory) DNA have been cloned in Escherichia coli and shown to express enzymatic activities related to xylan hydrolysis. Two of the 9 E. coli clones showed xylanase activity, one showed β-xylosidase activity as well as xylanase activity, and the other 6 showed β-xylosidase activity. On comparing the restriction maps of the xylanase and xylosidase genes cloned in this study with those reported so far, it was concluded that all the 9 clones carried hitherto unidentified genes. All the enzymes expressed in the E. coli clones were thermophilic.
- 社団法人日本農芸化学会の論文
- 1990-02-23
著者
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Kojima Yuzo
Medical Enzyme Division, Amano Enzyme Co., Ltd.
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Kojima Yuzo
Department Of Bioscience Faculty Of Bioresources Center For Molecular Biology And Genetics Mie Unive
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YOSHIKAWA Kazuhiro
Department of Pathology, Aichi Medical University School of Medicine
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Yoshikawa K
Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University
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Yoshikawa Kentaro
Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University
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Yoshikawa Kazuhiro
Department Of Bioscience Faculty Of Bioresources Mie University
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Sakka Kazuo
Department of Bioscience, Faculty of Bioresources, Mie University
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Shimada Kyo
Department of Bioscience, Faculty of Bioresources, Mie University
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Shimada Kyo
Department Of Bioscience Faculty Of Bioresources Mie University
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Kojima Y
Medical Enzyme Division Amano Enzyme Co. Ltd.
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Sakka Kazuo
Department Of Bioscience Faculty Of Bioresources Mie University
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YOSHIKAWA Kazuhiro
Department of Applied Chemistry, School of Engineering, Nagoya University
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SAKKA Kazuo
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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