Nucleotide Sequence of the Clostridium stercorarium xynA Gene Encoding Xylanase A : Identification of Catalytic and Cellulose Binding Domains
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概要
- 論文の詳細を見る
The nucleotides of the xynA gene of Clostridium stercorarium were sequenced. The structural gene consists of an open reading frame of 1533 bp encoding 511 amino acids with an M_r of 56,519. The signal peptide cleavage site was identified by comparison with the N-terminal amino acid sequence of the enzyme produced by a recombinant Escherichia coli. Xylanase A consists of a catalytic domain belonging to family G at the N-terminus and two direct repeats of about 90 amino acids with a short spacing at the C-terminus. Deletion analysis showed that the repeated sequences were responsible for binding the enzyme to Avicel and were not essential for catalytic activity. The catalytic domain of this enzyme is highly homologous to xylanase A of Clostridium acetobutylicum (identity: 69%) and xylanase B of Bacillus pumilus (identity: 64%).
- 社団法人日本農芸化学会の論文
- 1993-02-23
著者
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Kojima Yuzo
Medical Enzyme Division, Amano Enzyme Co., Ltd.
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Kojima Yuzo
Department Of Bioscience Faculty Of Bioresources Center For Molecular Biology And Genetics Mie Unive
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Ohmiya Kunio
Department Of Food Science And Technology Faculty Of Agriculture Nagoya University
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Sakka Kazuo
Department of Bioscience, Faculty of Bioresources, Mie University
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Karita Shu-ichi
Center for Molecular Biology and Genetics, Mie University
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Shimada Kyo
Department of Bioscience, Faculty of Bioresources, Mie University
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Shimada Kyo
Department Of Bioscience Faculty Of Bioresources Mie University
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Kojima Y
Medical Enzyme Division Amano Enzyme Co. Ltd.
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Sakka Kazuo
Department Of Bioscience Faculty Of Bioresources Mie University
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Ohmiya Kunio
Department Of Bioresources School Of Bioresources Mie University
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Kondo T
Tokyo Univ. Agriculture And Technol. Tokyo Jpn
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Kondo Tatsuki
Department of Bioscience, Faculty of Bioresources Center for Molecular Biology and Genetics, Mie Uni
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SAKKA Kazuo
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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