Cloning and Sequencing of the Gene Encoding Cyclodextrin Glucanotransferase from Bacillus sp. KC201
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概要
- 論文の詳細を見る
Bacillus sp. KC201 was newly isolated from soil as a high cyclodextrin glucanotransferase (CGTase) -producing-bacteria. The CGTase from Bacillus sp. KC201 converted 39.5% of soluble starch to cyclodextrins and the ratio of β- : γ-cyclodextrin produced was 6.2 : 1. No α-cyclodextrin was produced. A gene coding for the CGTase from Bacillus sp. KC201 was cloned into Escherichia coli, and its nucleotide sequence was determined. Starting at an ATG codon, there was an open reading frame composed of 2175bp (725 amino acids). The NH_2-terminal portion encoded a 51 amino acid-long signal peptide. The deduced amino acid sequence of the extracellular mature enzyme (674 amino acids) was identical with that of the CGTase from alkalophilic Bacillus sp. 1-1. Enzyme preparations purified from the culture supernatant of Bacillus sp. KC201 and from the intracellular fraction of the E. coli transformant had the same molecular weight. Amino acid sequences of both enzymes at the NH_2-terminal were consistent with each other. However, the enzymatic properties were slightly different.
- 社団法人日本生物工学会の論文
- 1992-12-25
著者
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Kojima Yuzo
Department Of Bioscience Faculty Of Bioresources Center For Molecular Biology And Genetics Mie Unive
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Ohmiya Kunio
Faculty Of Bioresources Mie University
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Kimura Tetsuya
National Center For Seeds And Seedlings
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Kitamoto Noriyuki
Food Research Center, Aichi Industrial Technology Institute
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Kojima Y
Medical Enzyme Division Amano Enzyme Co. Ltd.
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KITO YUKIO
Food Research Institute, Aichi Prefectural Government
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KIMURA TETSUYA
Food Research Institute, Aichi Prefectural Goverment
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Kitamoto N
Food Research Center Aichi Industrial Technology Institute
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Kitamoto Noriyuki
Food Research Center Aichi Industrial Technology Institute
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Kito Yukio
Food Research Institute Aichi Prefectural Government
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Kitamoto Noriyuki
Food Res. Center Aichi Ind. Tech. Inst.
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