Different Binding Specificities of S-Layer Homology Modules from Clostridium thermocellum AncA, Slp1, and Slp2

スポンサーリンク

概要

• 論文の詳細を見る

• S-layer homology (SLH) module polypeptides were derived from Clostridium thermocellum S-layer proteins Slp1 and Slp2 and cellulosome anchoring protein AncA as rSlp1-SLH, rSlp2-SLH, and rAncA-SLH respectively. Their binding specificities were investigated using C. thermocellum cell-wall preparations. rAncA-SLH associated with native peptidoglycan-containing sacculi from C. thermocellum, including both peptidoglycan and secondary cell wall polymers (SCWP), but not to hydrofluoric acid-extracted peptidoglycan-containing sacculi (HF-EPCS) lacking SCWPs, suggesting that SCWPs are responsible for binding with SLH modules of AncA. On the other hand, rSlp1-SLH and rSlp2-SLH associated with HF-EPCS, suggesting that these polypeptides had an affinity for peptidoglycan. A binding assay using a peptidoglycan fraction prepared from Escherichia coli cells definitely confirmed that rSlp1-SLH and rSlp2-SLH specifically interacted with peptidoglycan but not with SCWP.

• 社団法人 日本農芸化学会の論文
• 2006-07-23

著者

• KIMURA Tetsuya

  Faculty of Bioresources, Mie University

• Kajino T

  Special Research Laboratory Ii Toyota Central R&d Laboratories Inc.

• Kimura Tetsuya

  National Center For Seeds And Seedlings

• SAKKA Kazuo

  Faculty of Bioresources, Mie University

• Sakka K

  Graduate School Of Bioresources Mie Univ.

• Shima M

  Department Of Pediatrics Nara Medical University

• LI Huazhong

  School of Medicine, Southern Yangtze University

• ZHAO Guangshan

  Faculty of Bioresources, Mie University

• SAKKA Makiko

  Faculty of Bioresources, Mie University

• Kimura Tetsuya

  Department Of Science Kobe University

• Wamalwa Benson

  Analytical Chemistry Lab. Dep. Of Chemistry Univ. Of Nairobi

• Li Huazhong

  School Of Medicine Southern Yangtze University

• Sakka Kazuo

  Faculty Of Bioresources Mie University

• Zhao Guiwen

  Department Of Chemistry University Of Science And Technology Of China

• Sakka Makiko

  Graduate School Of Bioresources Mie Univ.

• Shibata M

  Univ. Tokyo Tokyo Jpn

• Karita S

  Graduate School Of Regional Innovation Studies Mie Univ.

• WAMALWA Benson

  Faculty of Bioresources, Mie University

• Kimura Tetsuya

  Graduate School Of Bioresources Mie University

• Sakka Makiko

  Faculty Of Bioresources Mie University

• Kimura Tetsuya

  Faculty Of Bioresources Mie University

• SHIBATA Masaru

  Department of Earth and Space Science, Faculty of ScienceOsaka University

• SHIBATA Masaru

  Department of Earth and Space Science Faculty of Science, Osaka University

• SHIBATA Masaru

  Department of Earth and Space Science, Faculty of Science Osaka University

• SHIBATA Masaru

  Graduate School of Arts and Sciences, University of Tokyo

• SIBATA Masaru

  Department of Physics, University of Illinois:Department of Earth and Space Science, Graduate School of Science, Osaha University

• SHIBATA Masaru

  Department of Earth and Space Science, Osaka University

関連論文

• Development of SSR Markers in Carnation (Dianthus caryophyllus)
• Round Shape Enlargement of the Yeast Spheroplast of Saccharomyces cerevisiae by HM-1 Toxin
• The Involvement of Two Specific Arginine Residues in the Action of HM-1 Killer Toxin was Deduced from Site-Directed Mutagenesis
• Characterization of Paenibacillus curdlanolyticus Intracellular Xylanase Xyn10B Encoded by the xyn10B Gene
• Molecular Cloning, Characterization, and Expression Analysis of the xynF3 Gene from Aspergillus oryzae(Microbiology & Fermentation Technology)
• Molecular Cloning, Overexpression, and Purification of a Major Xylanase from Aspergillus oryzae
• Analysis of the Promoter Activity of the Taka-Amylase Gene and the Phosphoglycerate Kinase Gene in a Shoyu-koji Mold Aspergillus oryzae KBN616
• Alanine-Scanning Mutagenesis of HM-1 Killer Toxin and the Essential Residues for Killing Activity
• Breeding of Yukirasha : Common Scab-Resistant Potato Variety for Table Stock
• Identification of a Catalytic Residue of Clostridium paraputrificum N-Acetyl-β-D-glucosaminidase Nag3A by Site-Directed Mutagenesis
• A Novel Thermophilic Pectate Lyase Containing Two Catalytic Modules of Clostridium stercorarium
• Detection of Hydrogen Gas-Producing Anaerobes in Refuse-Derived Fuel (RDF) Pellets
• Promoter of Arabidopsis thaliana Phosphate Transporter Gene Drives Root-Specific Expression of Transgene in Rice(Plant Biotechnology)
• Functions of Family-22 Carbohydrate-Binding Module in Clostridium thermocellum Xyn10C
• Cloning of the Ruminococcus albus cel5D and cel9A Genes Encoding Dockerin Module-containing Endoglucanases and Expression of cel5D in Escherichia coli
• Interaction between a Type-II Dockerin Domain and a Type-II Cohesin Domain from Clostridium thermocellum Cellulosome
• Analysis of Estrogen-Like Compounds in the Environment by High Performance Liquid Choromatography Bioassay (ENVIRONMENTAL BIOTECHNOLOGY)
• Sequencing and Expression of the Gene Encoding the Clostridium stercorarium β-Xylosidase Xyl43B in Escherichia coli
• Hydrogen Gas Generation from Refuse-derived Fuel (RDF) under Wet Conditions
• Cloning, Sequencing, and Expression of the Gene Encoding the Clostridium stercorarium α-Galactosidase Aga36A in Escherichia coli(Microbiology & Fermentation Technology)
• Electrotransformation of Clostridium paraputrificum M-21 with Some Plasmids(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• A New Type of β-N-Acetylglucosaminidase from Hydrogen-Producing Clostridium paraputrificum M-21(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• 3D11-3 In vitro growth and hydrolysis of cellulose and xylan by Emericella nidulans Rs1 in the presence of fermentation inhibitors
• Application of Microbial Genes to Recalcitrant Biomass Utilization and Environmental Conservation
• Stable Expression of a Thermostable Xylanase of Clostridium thermocellum in Cultured Tobacco Cells
• Isothermal Titration Calorimetric Studies on the Binding of a Family 6 Carbohydrate-binding Module of Clostridium thermocellum XynA with Xlylooligosaccharides(Biochemistry & Molecular Biology)
• 613 Cloning, Sequencing and Expression of The Gene Encoding the Clostridium stercorarium Pectate Lyase Pel9A in Escherichia coli
• Characterization of Clostridium paraputrificum Chitinase A from a Recombinant Escherichia coli
• Cloning, DNA Sequencing, and Expression of the β1,4-Mannanase Gene from a Marine Bacterium, Vibrio sp. Strain MA-138
• Sequence of the Clostridium thermocellum Mannanase Gene man26B and Characterization of the Translated Product
• Cloning and Sequencing of a Novel Endo-1,4-β-Glucanase Gene from Ruminococcus albus
• Effect of Pressure on the Association States of Enzyme-treated Caseins
• Characterization of Bacillus halodurans α-Galactosidase Mel4A Encoded by the mel4A Gene (BH2228)
• Isolation and expression analysis of phosphate transporter genes from Eucalyptus camaldulensis
• DNA Profiling of Fresh and Processed Fruits in Pear
• Identification of Quince Varieties Using SSR Markers Developed from Pear and Apple
• Identification of Asian Pear Varieties by SSR Analysis
• Molecular Cloning of Xylanase Gene xynG1 from Aspergillus oryzae KBN 616,a Shoyu Koji Mold,and Analysis of Its Expression
• Estimation of Ploidy Levels and Breeding Backgrounds in Pot Carnation Cultivars Using Flow Cytometry and SSR Markers
• Genetic linkage map of the Japanese pear 'Housui' identifying three homozygous genomic regions
• Identification of Parent-offspring Relationships in 55 Japanese Pear Cultivars Using S-RNase Allele and SSR markers
• Integrated Reference Genetic Linkage Maps of Pear Based on SSR and AFLP Markers
• Physiological Responses to Maximal Treadmill and Deep Water Running in the Young and the Middle Aged Males
• Maximal Physiological Responses to Deep Water Running at Thermoneutral Temperature
• Purification and Characterization of the Family J Catalytic Domain Derived from the Clostridium thermocellum Endoglucanase CelJ
• Specific Adsorption of Clostridium stercorarium Xylanase to Amorphous Cellulose and Its Desorption by Cellobiose
• Robust Speed Control of Ultrasonic Motor Based on H_∞ Control with Repetitive Compensator
• Cloning and Sequencing of the Gene Encoding Chitinase ChiA from Xanthomonas sp.Strain AK and Some Properties of ChiA
• Metalloregulatory Properties of the ArsR and ArsD Repressors of Acidiphilium multivorum AIU 301
• Stable Production of Thermotolerant Xylanase B of Clostridium stercorarium in Transgenic Tobacco and Rice
• Two Proteins with Diaphorase Activity from Clostridium thermocellum and Moorella thermoacetica
• Cloning and Expression of a Clostridium kluyveri Gene Responsible for Diaphorase Activity
• Characterization of a Dihydrolipoyl Dehydrogenase Having Diaphorase Activity of Clostridium kluyveri
• Detection of Anaerobic Ammonium-Oxidizing Bacteria in Ago Bay Sediments
• Tri-/Hexanucleotide Microsatellite Markers in Peach Derived from Enriched Genomic Libraries and Their Application in Rosaceae
• Effect of Family 22 Carbohydrate-Binding Module on the Thermostability of Xyn10B Catalytic Module from Clostridium stercorarium
• Comparison of Microbial Consortia in Refuse-Derived Fuel (RDF) Preparations between Japan and Germany
• 1G14-5 Properties of Bacillus haloduarans Xyn11A Expressed in Kluyveromyces lactis
• Different Binding Specificities of S-Layer Homology Modules from Clostridium thermocellum AncA, Slp1, and Slp2
• Enzymatic Properties of Two Catalytic Modules of Clostridium stercorarium Pectate Lyase Pel9A
• Molecular Cloning of Xylanase Gene xynG1 from Aspergillus oryzae KBN 616, a Shoyu Koji Mold, and Analysis of Its Expression
• Productino of HM-1 Killer Toxin in Saccharomyces cerevisiae Transformed with the PDR4 Gene and δ-Sequence-Mediated Multi-Integration System
• The Nitrate Reductase Gene from a Shoyu Koji Mold, Aspergillus oryzae KBN616
• Structural Relationships among Killer Toxins Secreted from the Killer Strains of the Genus Williopsis
• Purification and Characterization of Xylanase A from CIostridium stercorarium F-9 and a Recombinant Escherichia coli
• Cloning and Sequencing of the Gene Encoding Cyclodextrin Glucanotransferase from Bacillus sp. KC201
• Purification, Characterization, and Molecular Cloning of Acidophilic Xylanase from Penicillium sp.40
• Characterization of the Cellulolytic Complex (Cellulosome) from Ruminococcus albus
• Sequence of egV and Properties of EgV, a Ruminococcus albus Endoglucanase Containing a Dockerin Domain
• Expression of a Bacterial Endoglucanase Gene in Tobacco Increases Digestibility of Its Cell Wall Fibers
• Adsorption of Clostridium stercorarium Xylanase A to Insoluble Xylan and the Importance of the CBDs to Xylan Hydrolysis
• High Expression of the Xylanase B Gene from Clostridium stercorarium in Tobacco Cells
• 478 Affinity of cellulose-binding domains of Clostridium stercorarium xylanase A for xylan and soluble saccharides and effect of connection of CBD to an endoglucanase on its enzyme activity
• Functions of Family-22 Carbohydrate-Binding Modules in Clostridium josui Xyn10A
• 3C11-1 Functions of family-22 carbohydrate-binding module in Clostridium josui Xyn10A
• Function of the Family-9 and Family-22 Carbohydrate-Binding Modules in a Modular β-1,3-1,4-Glucanase/Xylanase Derived from Clostridium stercorarium Xyn10B
• Analysis of a Clostridium josui Cellulase Gene Cluster Containing the man5A Gene and Characterization of Recombinant Man5A
• Importance of the Carbohydrate-Binding Module of Clostridium stercorarium Xyn10B to Xylan Hydrolysis
• Characterization of a Dihydrolipoyl Dehydrogenase Having Diaphorase Activity of Clostridium kluyveri
• Two Proteins with Diaphorase Activity from Clostridium thermocellum and Moorella thermoacetica
• Cloning and Expression of a Clostridium kluyveri Gene Responsible for Diaphorase Activity
• Characterization of Paenibacillus curdlanolyticus Intracellular Xylanase Xyn10B Encoded by the xyn10B Gene
• Stable Production of Thermotolerant Xylanase B of Clostridium stercorarium in Transgenic Tobacco and Rice
• Stable Expression of the Chlorocatechol Dioxygenase Gene from Ralstonia eutropha NH9 in Hybrid Poplar Cells
• Characterization of Bacillus halodurans α-Galactosidase Me14A Encoded by the mel4A Gene (BH2228)
• Detection of Anaerobic Ammonium-Oxidizing Bacteria in Ago Bay Sediments
• Thermal Unfolding and Modular Architecture of Clostridium stercorarium Xyn10B
• High-Level Heterologous Expression of Bacillus halodurans Putative Xylanase Xyn11A (BH0899) in Kluyveromyces lactis
• Expression of Hydrogenase Gene in Clostridium Paraputrificum for Enhancing Hydrogen Gas Production (Proceedings of the Asia-Pacific Conference on Sustainable Energy and Environmental Technologies)
• Conversion of Chitinous Wastes to Hydrogen Gas by Clostridium paraputrificum M-21
• Phylogeography of the endangered tideland snail Batillaria zonalis in the Japanese and Ryukyu Islands
• Phylogenetic Relationships between the Tideland Snails Batillaria flectosiphonata in the Ryukyu Islands and B. multiformis in the Japanese Islands(Animal Diversity and Evolution)
• Identification and Characterization of Clostridium paraputrificum M-21, a Chitinolytic, Mesophilic and Hydrogen-Producing Bacterium
• Cloning, Sequencing, and Expression of the Gene Encoding the Clostridium stercorarium Xylanase C in Escherichia coli
• Expression of a Ruminococcus albus Cellulase Gene in Tobacco Suspension Cells
• Purification and Some Properties of a Chitinase from Xanthomonas sp. Strain AK
• Cloning, Sequencing, and Expression of the Gene Encoding a Cell-bound Multi-domain Xylanase from Clostridium josui, and Characterization of the Translated Product
• Molecular Breeding of Cellulolytic Microbes, Plants, and Animals for Biomass Utilization
• Purification and Characterization of the Clostridium josui Porphobilinogen Deaminase Encoded by the hemC Gene from a Recombinant Escherichia coil
• Purification of the Ruminococcus albus Endoglucanase IV Using a Cellulose-Binding Domain as an Affinity Tag

もっと見る 閉じる

スポンサーリンク