Round Shape Enlargement of the Yeast Spheroplast of Saccharomyces cerevisiae by HM-1 Toxin

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概要

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• The effects of HM-1 killer toxin (HM-1) on yeast spheroplasts of Saccharomyces cerevisiae were examined under osmotically stabilized conditions. Prolonged incubation of spheroplasts in nutrient-rich media resulted in an increase in volume, accompanied by aberrant morphological changes. By contrast, spheroplasts were enlarged, maintaining a round shape, when incubated in HM-1 media. The required 50% effective dose of HM-1 was as low as 2.2×10^<-8> M, and this effect by HM-1 was specific to yeast sensitive to HM-1. Some parts of the enlarged spheroplasts were stable, but the round shape was deformed as HM-1 was removed from the medium. In both the control and HM-1-treated spheroplasts, the total protein and DNA content were increased by approximately three and four times in response to their incubations, respectively. Cytochemical analysis by 4'6-diamidino-2-phenylindol (DAPI) staining showed multiple nuclei. Consistently, actin patches of cells were evenly distributed in both the control and HM-1-treated spheroplasts. A similar enlargement of spheroplasts was observed with lipophilic antifungal compounds, aculeacin A and papulacandin B, but the effects were distinct from those of HM-1 because the spheroplasts resulted in lysis after a long incubation. The molecular mechanism(s) behind this unique observation remains to be studied, but it is clear that HM-1 is an excellent tool for studying yeast cell biology.

• 社団法人日本薬学会の論文
• 2002-08-01

著者

• FURUICHI Yasuhiro

  GeneCare (previously AGENE) Research Institute

• Furuichi Y

  Genecare Research Institute

• Furuichi Yasuhiro

  新潟薬科大学 薬学部生化学

• Furuichi Yasuhiro

  ジーンケア研究所

• Furuichi Yasuhiro

  Genecare Research Institute

• Furuichi Yasuhiro

  Genecare Research Institute Co. Ltd.

• KOMIYAMA Tadazumi

  Department of Biochemistry, Niigata College of Pharmacy

• KIMURA Tetsuya

  Faculty of Bioresources, Mie University

• Furuichi Yasuhiro

  Genecare Research Institute Co. Ltd.:core Research For Evolution Science And Technology Japan Scienc

• Kimura T

  Okayama Univ. Okayama Jpn

• Koide T

  Laboratory Of Pharmacognosy Graduate School Of Pharmaceutical Sciences Nagoya City University

• Komiyama T

  Department Of Biochemistry Faculty Of Pharmaceutical Sciences Niigata University Of Pharmacy And App

• Komiyama Tadazumi

  Department Of Biochemistry Faculty Of Pharmaceutical Sciences Niigata University Of Pharmacy And App

• Kimura Tetsuya

  Faculty Of Bioresources Mie University

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