Functions of Family-22 Carbohydrate-Binding Modules in Clostridium josui Xyn10A

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概要

• 論文の詳細を見る

• Clostridium josui xylanase Xyn10A is a modular enzyme comprising two family-22 carbohydrate-binding modules (CBMs), a family-10 catalytic module (CM), a family-9 CBM, and two S-layer homologous modules, consecutively from the N-terminus. To study the functions of the family-22 CBMs, truncated derivatives of Xyn10A were constructed: a recombinant CM polypeptide (rCM), a family-22 CBM polypeptide (rCBM), and a polypeptide composed of the family-22 CBMs and CM (rCBM-CM). Recombinant proteins were characterized by enzyme and binding assays. rCBM-CM showed the highest activity toward xylan and weak activity toward some polysaccharides such as barley β-glucan and carboxymethyl-cellulose. Although rCBM showed an affinity for insoluble and soluble xylan as well as barley β-glucan and Avicel in qualitative binding assays, removal of the CBMs negligibly affected the catalytic activity and thermostability of the CM.

• 社団法人 日本農芸化学会の論文
• 2005-12-23

著者

• KIMURA Tetsuya

  Faculty of Bioresources, Mie University

• Kajino T

  Special Research Laboratory Ii Toyota Central R&d Laboratories Inc.

• Kimura Tetsuya

  National Center For Seeds And Seedlings

• Ali Ehsan

  Fac. Biores. Mie Univ.

• Karita Shuichi

  Faculty of Bioresources, Mie University

• SAKKA Kazuo

  Faculty of Bioresources, Mie University

• Sakka K

  Graduate School Of Bioresources Mie Univ.

• Shima M

  Department Of Pediatrics Nara Medical University

• ZHAO Guangshan

  Faculty of Bioresources, Mie University

• SAKKA Makiko

  Faculty of Bioresources, Mie University

• ALI Ehsan

  Faculty of Bioresources, Mie University

• Sakka Kazuo

  Faculty Of Bioresources Mie University

• Zhao Guiwen

  Department Of Chemistry University Of Science And Technology Of China

• Sakka Makiko

  Graduate School Of Bioresources Mie Univ.

• Shibata M

  Univ. Tokyo Tokyo Jpn

• Karita S

  Graduate School Of Regional Innovation Studies Mie Univ.

• Karita Shuichi

  Faculty Of Bioresources Mie University

• ARAKI Rie

  Faculty of Bioresources, Mie University

• Kimura Tetsuya

  Graduate School Of Bioresources Mie University

• Araki Rie

  Sustainable Resource Sciences Graduate School Of Bioresources Mie University

• Sakka Makiko

  Faculty Of Bioresources Mie University

• Kimura Tetsuya

  Faculty Of Bioresources Mie University

• SHIBATA Masaru

  Department of Earth and Space Science, Faculty of ScienceOsaka University

• SHIBATA Masaru

  Department of Earth and Space Science Faculty of Science, Osaka University

• SHIBATA Masaru

  Department of Earth and Space Science, Faculty of Science Osaka University

• SHIBATA Masaru

  Graduate School of Arts and Sciences, University of Tokyo

• SIBATA Masaru

  Department of Physics, University of Illinois:Department of Earth and Space Science, Graduate School of Science, Osaha University

• SHIBATA Masaru

  Department of Earth and Space Science, Osaka University

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