Sequence of the Clostridium thermocellum Mannanase Gene man26B and Characterization of the Translated Product

スポンサーリンク

概要

• 論文の詳細を見る

• The man26B gene of Clostridium thermocellum strain F1 was found in pKS305,which had been selected as a recombinant plasmid conferring endoglucanase activity on Escherichia coli. The open reading frame of man26B consists of 1,773 nucleotides encoding a protein of 591 amino acids with a predicted molecular weight of 67,047. Man26B is a modular enzyme composed of an N-terminal signal peptide and three domains in the following order : a mannan-binding domain, a family 26 mannanase domain, and a dockerin domain responsible for cellulosome assembly. We found that this gene was a homologue of the man26A gene of C. thermocellum strain YS but that there were insertion or deletion mutations that caused a frame-shift mutation affecting a stretch of 26 amino acids in the catalytic domain. Man26B devoid of the dockerin domain was constructed and purified from a recombinant E.coli, and its enzyme properties were examined. Immunological analysis indicated that Man26B was a catalytic component of the C.thermocellum F1 cellulosome.

• 社団法人日本農芸化学会の論文
• 2001-03-23

著者

• Ohmiya Kunio

  Faculty Of Bioresources Mie University

• Araki T

  Faculty Of Bioresources Mie University

• Arai Takamitsu

  Faculty Of Bioresources And Center For Molecular Biology And Genetics Mie University

• KIMURA Tetsuya

  Faculty of Bioresources, Mie University

• Kajino T

  Special Research Laboratory Ii Toyota Central R&d Laboratories Inc.

• Kurokawa Junji

  Faculty Of Bioresources Mie University

• Aburatani Takeshi

  Faculty Of Bioresources Mie University

• SAKKA Kazuo

  Faculty of Bioresources, Mie University

• KARITA Shuichi

  Center for Molecular Biology and Genetics Mie University

• Kurokawa Junji

  Fac. Biores., Mie Univ.

• HEMJINDA Eiakalak

  Faculty of Bioresources, Mie University

• Sakka Kazuo

  Faculty Of Bioresources Mie University

• Arai T

  National Inst. Of Fruit Tree Sci. Nagasaki Jpn

• Hemjinda Eiakalak

  Faculty Of Bioresources Mie University

• Kimura Tetsuya

  Graduate School Of Bioresources Mie University

• Kimura Tetsuya

  Faculty Of Bioresources Mie University

関連論文

• Development of SSR Markers in Carnation (Dianthus caryophyllus)
• Round Shape Enlargement of the Yeast Spheroplast of Saccharomyces cerevisiae by HM-1 Toxin
• The Involvement of Two Specific Arginine Residues in the Action of HM-1 Killer Toxin was Deduced from Site-Directed Mutagenesis
• Characterization of Paenibacillus curdlanolyticus Intracellular Xylanase Xyn10B Encoded by the xyn10B Gene
• Molecular Cloning, Characterization, and Expression Analysis of the xynF3 Gene from Aspergillus oryzae(Microbiology & Fermentation Technology)
• Molecular Cloning, Overexpression, and Purification of a Major Xylanase from Aspergillus oryzae
• Analysis of the Promoter Activity of the Taka-Amylase Gene and the Phosphoglycerate Kinase Gene in a Shoyu-koji Mold Aspergillus oryzae KBN616
• Alanine-Scanning Mutagenesis of HM-1 Killer Toxin and the Essential Residues for Killing Activity
• Breeding of Yukirasha : Common Scab-Resistant Potato Variety for Table Stock
• Synthesis and Biological Activity of New Water-soluble Cytokinins
• Synthesis of Squamostanal-A
• Synthesis of Two Possible Diastereoisomers of the Epoxy Lactone Proposed for Epoxyrollin A
• 2P-079 グルコアミラーゼのデンプン結合ドメインの安定性とリフォールディング過程に対するジスルフィド結合の効果(蛋白質・物性(2),第46回日本生物物理学会年会)
• Identification of a Catalytic Residue of Clostridium paraputrificum N-Acetyl-β-D-glucosaminidase Nag3A by Site-Directed Mutagenesis
• A Novel Thermophilic Pectate Lyase Containing Two Catalytic Modules of Clostridium stercorarium
• Detection of Hydrogen Gas-Producing Anaerobes in Refuse-Derived Fuel (RDF) Pellets
• Promoter of Arabidopsis thaliana Phosphate Transporter Gene Drives Root-Specific Expression of Transgene in Rice(Plant Biotechnology)
• Functions of Family-22 Carbohydrate-Binding Module in Clostridium thermocellum Xyn10C
• Cloning of the Ruminococcus albus cel5D and cel9A Genes Encoding Dockerin Module-containing Endoglucanases and Expression of cel5D in Escherichia coli
• Interaction between a Type-II Dockerin Domain and a Type-II Cohesin Domain from Clostridium thermocellum Cellulosome
• Analysis of Estrogen-Like Compounds in the Environment by High Performance Liquid Choromatography Bioassay (ENVIRONMENTAL BIOTECHNOLOGY)
• Sequencing and Expression of the Gene Encoding the Clostridium stercorarium β-Xylosidase Xyl43B in Escherichia coli
• Hydrogen Gas Generation from Refuse-derived Fuel (RDF) under Wet Conditions
• Cloning, Sequencing, and Expression of the Gene Encoding the Clostridium stercorarium α-Galactosidase Aga36A in Escherichia coli(Microbiology & Fermentation Technology)
• Electrotransformation of Clostridium paraputrificum M-21 with Some Plasmids(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• A New Type of β-N-Acetylglucosaminidase from Hydrogen-Producing Clostridium paraputrificum M-21(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• 3D11-3 In vitro growth and hydrolysis of cellulose and xylan by Emericella nidulans Rs1 in the presence of fermentation inhibitors
• Application of Microbial Genes to Recalcitrant Biomass Utilization and Environmental Conservation
• Stable Expression of a Thermostable Xylanase of Clostridium thermocellum in Cultured Tobacco Cells
• Isothermal Titration Calorimetric Studies on the Binding of a Family 6 Carbohydrate-binding Module of Clostridium thermocellum XynA with Xlylooligosaccharides(Biochemistry & Molecular Biology)
• 613 Cloning, Sequencing and Expression of The Gene Encoding the Clostridium stercorarium Pectate Lyase Pel9A in Escherichia coli
• Characterization of Clostridium paraputrificum Chitinase A from a Recombinant Escherichia coli
• Cloning, DNA Sequencing, and Expression of the β1,4-Mannanase Gene from a Marine Bacterium, Vibrio sp. Strain MA-138
• Sequence of the Clostridium thermocellum Mannanase Gene man26B and Characterization of the Translated Product
• Cloning and Sequencing of a Novel Endo-1,4-β-Glucanase Gene from Ruminococcus albus
• Effect of Pressure on the Association States of Enzyme-treated Caseins
• Purification and Properties of the Endo-1,4-β-Glucanase III from Ruminococcus albus
• Purification and Properties of the Endo-1,4-β-Glucanase from Ruminococcus albus and Its Gene Product in Escherichia coli
• Characterization of Bacillus halodurans α-Galactosidase Mel4A Encoded by the mel4A Gene (BH2228)
• Isolation and expression analysis of phosphate transporter genes from Eucalyptus camaldulensis
• DNA Profiling of Fresh and Processed Fruits in Pear
• Identification of Quince Varieties Using SSR Markers Developed from Pear and Apple
• Identification of Asian Pear Varieties by SSR Analysis
• Thermostable β-Galactosidase from Bacillus acidocaldarius and Its Immobilization
• Purification and Some Properties of Alkaline Proteinases from Cephalosporium sp. KM388(Biological Chemistry)
• Molecular Cloning of Xylanase Gene xynG1 from Aspergillus oryzae KBN 616,a Shoyu Koji Mold,and Analysis of Its Expression
• Estimation of Ploidy Levels and Breeding Backgrounds in Pot Carnation Cultivars Using Flow Cytometry and SSR Markers
• Genetic linkage map of the Japanese pear 'Housui' identifying three homozygous genomic regions
• Identification of Parent-offspring Relationships in 55 Japanese Pear Cultivars Using S-RNase Allele and SSR markers
• Integrated Reference Genetic Linkage Maps of Pear Based on SSR and AFLP Markers
• Physiological Responses to Maximal Treadmill and Deep Water Running in the Young and the Middle Aged Males
• Maximal Physiological Responses to Deep Water Running at Thermoneutral Temperature
• Purification and Characterization of the Family J Catalytic Domain Derived from the Clostridium thermocellum Endoglucanase CelJ
• Specific Adsorption of Clostridium stercorarium Xylanase to Amorphous Cellulose and Its Desorption by Cellobiose
• 123 Characterization of An Endoglucanase Gene from Clostridium thermocellum and Its Translated Product
• Pectin Methylesterase Gene (pmeA) from Aspergillus oryzae KBN616 : Its Sequence Analysis and Overexpression, and Characterization of the Gene Product
• A Second Pectin Lyase Gene (pel2)from Aspergillus oryzae KBN616 : Its Sequence Analysis and Overexpression, and Characterization of the Gene Products
• Sequence Analysis and Overexpression of a Pectin Lyase Gene (pel1) from Aspergillus oryzae KBN616
• Purification and Characterization of the Overexpressed Aspergillus oryzae Xylanase, XynF1
• Robust Speed Control of Ultrasonic Motor Based on H_∞ Control with Repetitive Compensator
• Cloning and Sequencing of the Gene Encoding Chitinase ChiA from Xanthomonas sp.Strain AK and Some Properties of ChiA
• Metalloregulatory Properties of the ArsR and ArsD Repressors of Acidiphilium multivorum AIU 301
• Substrate Specificity of Alkaline Proteases from Cephalosporium sp. KM388
• Expression of a celE Gene from Clostridium thermocellum in Bacillus
• Construction of Genetically Marked Ruminococcus albus Strains and Conjugal Transfer of Plasmid pAMβ1 into Them
• Structure of a β-Glucosidase Gene from Ruminococcus albus and Properties of the Translated Product
• An Investigation of the pH-Activity Relationships of Cex, a Family 10 Xylanase from Cellulomonas fimi : Xylan Inhibition and the Influence of Nitro-Substituted Aryl-β-D-Xylobiosides on Xylanase Activity
• Stable Production of Thermotolerant Xylanase B of Clostridium stercorarium in Transgenic Tobacco and Rice
• Two Proteins with Diaphorase Activity from Clostridium thermocellum and Moorella thermoacetica
• Cloning and Expression of a Clostridium kluyveri Gene Responsible for Diaphorase Activity
• Effect of Family 22 Carbohydrate-Binding Module on the Thermostability of Xyn10B Catalytic Module from Clostridium stercorarium
• Comparison of Microbial Consortia in Refuse-Derived Fuel (RDF) Preparations between Japan and Germany
• 1G14-5 Properties of Bacillus haloduarans Xyn11A Expressed in Kluyveromyces lactis
• Different Binding Specificities of S-Layer Homology Modules from Clostridium thermocellum AncA, Slp1, and Slp2
• Enzymatic Properties of Two Catalytic Modules of Clostridium stercorarium Pectate Lyase Pel9A
• The Nitrate Reductase Gene from a Shoyu Koji Mold, Aspergillus oryzae KBN616
• Cloning and Sequencing of the Gene Encoding Cyclodextrin Glucanotransferase from Bacillus sp. KC201
• Purification, Characterization, and Molecular Cloning of Acidophilic Xylanase from Penicillium sp.40
• Characterization of the Cellulolytic Complex (Cellulosome) from Ruminococcus albus
• Sequence of egV and Properties of EgV, a Ruminococcus albus Endoglucanase Containing a Dockerin Domain
• Thermal Unfolding and Modular Architecture of Clostridium stercorarium Xyn10B
• Process of Thermal Denaturation of Xylanase (XynB) from Clostridium stercorarium F-9
• Synthesis of (-)-Muricatacin
• Expression of a Bacterial Endoglucanase Gene in Tobacco Increases Digestibility of Its Cell Wall Fibers
• Adsorption of Clostridium stercorarium Xylanase A to Insoluble Xylan and the Importance of the CBDs to Xylan Hydrolysis
• Functions of Family-22 Carbohydrate-Binding Modules in Clostridium josui Xyn10A
• Function of the Family-9 and Family-22 Carbohydrate-Binding Modules in a Modular β-1,3-1,4-Glucanase/Xylanase Derived from Clostridium stercorarium Xyn10B
• Importance of the Carbohydrate-Binding Module of Clostridium stercorarium Xyn10B to Xylan Hydrolysis
• Thermal Unfolding and Modular Architecture of Clostridium stercorarium Xyn10B
• Conversion of Chitinous Wastes to Hydrogen Gas by Clostridium paraputrificum M-21
• Phylogeography of the endangered tideland snail Batillaria zonalis in the Japanese and Ryukyu Islands
• Phylogenetic Relationships between the Tideland Snails Batillaria flectosiphonata in the Ryukyu Islands and B. multiformis in the Japanese Islands(Animal Diversity and Evolution)
• Identification and Characterization of Clostridium paraputrificum M-21, a Chitinolytic, Mesophilic and Hydrogen-Producing Bacterium
• Expression of a Ruminococcus albus Cellulase Gene in Tobacco Suspension Cells
• Purification and Some Properties of a Chitinase from Xanthomonas sp. Strain AK
• Cloning, Sequencing, and Expression of the Gene Encoding a Cell-bound Multi-domain Xylanase from Clostridium josui, and Characterization of the Translated Product
• Molecular Breeding of Cellulolytic Microbes, Plants, and Animals for Biomass Utilization
• Cloning of the celB Gene Encoding Endo-1,4-β-Glucanase-2 from Clostridium josui in Escherichia coli and the Properties of the Translated Product
• Purification and Characterization of the Clostridium josui Porphobilinogen Deaminase Encoded by the hemC Gene from a Recombinant Escherichia coil
• Nucleotide Sequence of the Clostridium stercorarium xynB Gene Encoding an Extremely Thermostable Xylanase, and Characterization of the Translated Product

もっと見る 閉じる

スポンサーリンク