Purification and Some Properties of Alkaline Proteinases from Cephalosporium sp. KM388(Biological Chemistry)

スポンサーリンク

概要

• 論文の詳細を見る

• Cephalosporium sp. KM388 produced two kinds of extracellular alkaline proteinases (C and D) in complex medium. Proteinases C and D were purified 263 and 195-fold, respectively, to an electrophoretically homogeneous state from the culture broth by hydrophobic adsorption on Butyl-Toyopearl 650M with 30% saturated ammonium sulfate and chromatographies on DEAE-Sepharose CL-6B, DEAE-Toyopearl 650M, CM-Sepharose CL-6B, and Sephadex G-75. The molecular weights of proteinases C and D were 22,000 and 24,000, respectively, by gel filtration. The isoelectric points were observed as pI>10.5 for proteinase C and pI=3.8 for proteinase D. The pH optima for the proteolytic activity of proteinases C and D were 11 and 10, respectively. Proteinase C was unstable below pH 10 but was stabilized by Ca^<2+> or Mg^<2+>. Proteinase D was stable above pH 7. Proteinase C was inhibited only Hg^<2+>, but proteinase D was inhibited by Mn^<2+> and Zn^<2+> in addition to Hg^<2+>. Both proteinases were inhibited strongly by chymostatin, weakly by DFP and PMSF, but little by PCMB, MIA, EDTA, and SDS. These enzymes showed very high activity against BTEE but low activities against BAEE and TAME as well as Bz-ala-OMe.

• 社団法人日本農芸化学会の論文
• 1987-11-23

著者

• Araki T

  Faculty Of Bioresources Mie University

• Arai Takamitsu

  Faculty Of Bioresources And Center For Molecular Biology And Genetics Mie University

• Aburatani Takeshi

  Faculty Of Bioresources Mie University

• TSUCHIYA Katsumi

  Faculty of Pharmaceutical Sciences, Josai University

• KIMURA Tetsu

  Faculty of Pharmaceutical Sciences, Josai University

• ARAI Tsutomu

  Faculty of Engineering, Toyo University

• Seki K

  Chiba Univ. Chiba Jpn

• Kimura T

  Osaka Kyoiku Univ. Osaka Jpn

• Seki Kiyohiko

  Department Of Applied Biological Sciences Faculty Of Agriculture Saga University

• Arai T

  National Inst. Of Fruit Tree Sci. Nagasaki Jpn

• Arai Tsutomu

  Faculty Of Engineering Toyo University

• Kimura Tetsu

  Faculty Of Pharmaceutical Sciences Josai University

• Tsuchiya K

  Josai Univ. Saitama Jpn

• SEKI Kazuyuki

  Faculty of Pharmaceutical Sciences, Josai University

• Tsuchiya K

  Univ. Tokushima Tokushima Jpn

• Tsuchiya Katsumi

  Faculty Of Pharmaceutical Sciences Josai University

• Seki Kazuyuki

  Faculty Of Pharmaceutical Sciences Josai University

• KIMURA Tetsu

  Faculty of Bioresources, Mie University

関連論文

• Molecular Cloning, Characterization, and Expression Analysis of the xynF3 Gene from Aspergillus oryzae(Microbiology & Fermentation Technology)
• Molecular Cloning, Overexpression, and Purification of a Major Xylanase from Aspergillus oryzae
• Analysis of the Promoter Activity of the Taka-Amylase Gene and the Phosphoglycerate Kinase Gene in a Shoyu-koji Mold Aspergillus oryzae KBN616
• Production of Recombinant Human Monoclonal Antibody Using ras-Amplified BHK-21 Cells in a Protein-free Medium
• Synthesis and Biological Activity of New Water-soluble Cytokinins
• Synthesis of Squamostanal-A
• Synthesis of Two Possible Diastereoisomers of the Epoxy Lactone Proposed for Epoxyrollin A
• Cloning and Expression of an Intracellular Alkaline Protease Gene from Alkalophilic Thermoactinomyces sp. HS682
• Generation of Monoclonal Antibodies for Detection of a Kuchiziro Fish Virus of Tiger Puffer (Takifugu rubripes)
• Changes in Specificities of Human Monoclonal Antibodies by Introduction of Drug-resistance Gene into Hybridomas(Biological Chemistry)
• 2P-079 グルコアミラーゼのデンプン結合ドメインの安定性とリフォールディング過程に対するジスルフィド結合の効果(蛋白質・物性(2),第46回日本生物物理学会年会)
• Isothermal Titration Calorimetric Studies on the Binding of a Family 6 Carbohydrate-binding Module of Clostridium thermocellum XynA with Xlylooligosaccharides(Biochemistry & Molecular Biology)
• Cloning, DNA Sequencing, and Expression of the β1,4-Mannanase Gene from a Marine Bacterium, Vibrio sp. Strain MA-138
• Production of a Recombinant Human Monoclonal Antibody Using a Novel Hollow Fiber Bioreactor System
• Sequence of the Clostridium thermocellum Mannanase Gene man26B and Characterization of the Translated Product
• Measurement of Thermal Diffusivity by Laser Pulse
• Relation of Laser Induced Ion Energy to Laser Power
• Primary Metabolic Pathway of Methylamine in Methylophaga sp. AA-30(Microbiology & Fermentation Industry)
• Sodium-dependent Growth of a Marine Methylotrophic Bacterium, Methylophaga sp. AA-30(Microbiology & Fermentation Industry)
• Use of Short-chain Amines and Amino Acids as Sole Sources of Nitrogen in a Marine Methylotrophic Bacterium, Methylophaga sp. AA-30(Microbiology & Fermentation Industry)
• Continuous Production of Maltose Using a Dual Immobilized Enzyme System(Biological Chemistry)
• Immobilization of Exo-maltotetraohydrolase and Pullulanase(Biological Chemistry)
• Purification and Some Properties of Alkaline Proteinases from Cephalosporium sp. KM388(Biological Chemistry)
• Cloning and Nucleotide Sequence of the Inulin Fructotransferase (DFA I-Producing)Gene of Arthrobacter globformis S14-3
• Substrate Specificity of Alkaline Proteases from Cephalosporium sp. KM388
• Purification and Properties of a Novel Inulin Fructotransferase(DFA I-Producing) from Arthrobacter globiformis S14-3(Microbiology & Fermentation Industry)
• Purification and Properties of Inulin Fructotransferase (Depolymerizing) from Arthrobacter globiformis C11-1(Microbiology & Fermentation Industry)
• Effect of Family 22 Carbohydrate-Binding Module on the Thermostability of Xyn10B Catalytic Module from Clostridium stercorarium
• Decrease of Protease Activity by the Addition of Glucose to the Culture of Cephalosporium sp.
• Thermal Unfolding and Modular Architecture of Clostridium stercorarium Xyn10B
• Process of Thermal Denaturation of Xylanase (XynB) from Clostridium stercorarium F-9
• Synthesis of (-)-Muricatacin
• Thermal Unfolding and Modular Architecture of Clostridium stercorarium Xyn10B
• Purification and Characterization of a Thermostable Alkaline Protease from Alkalophilic Thermoactinomyces sp. HS682
• Production of Thermostable Alkaline Protease by Alkalophilic Thermoactinomyces sp. HS682(Microbiology & Fermentation Industry)
• Production and Purification of Alkaline Protease Inhibitors of Streptomyces sp.(Microbiology & Fermentation Industry)
• Prohisin, New Thiol Protease Inhibitor Produced by Cephalosporium sp. KM 388

もっと見る 閉じる

スポンサーリンク