Analysis of the Promoter Activity of the Taka-Amylase Gene and the Phosphoglycerate Kinase Gene in a Shoyu-koji Mold Aspergillus oryzae KBN616

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概要

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• Promoter activity of the genes encoding Taka-amylase A and phosphoglycerate kinase of Aspergillus oryzae was analyzed using Escherichia coli β-glucuronidase as a reporter in a shoyu-koji mold A. oryzae KBN616. Assay of the β-glucuronidase extracted from the mycelia of the transformants grown in the media containing different carbon sources suggests that expression of the Taka-amylase A gene was not induced by starch in A. oryzae KBN616. Analysis of proteins in the culture supernatant of A. oryzae KBN616 after cultivation in starch medium supports this result. The phosphoglycerate kinase gene of A. oryzae KBN616 was shown to be expressed constitutively in the medium containing glucose and starch.

• 社団法人　日本食品科学工学会の論文
• 2000-02-01

著者

• Ohmiya Kunio

  Faculty Of Bioresources Mie University

• Araki T

  Faculty Of Bioresources Mie University

• Arai Takamitsu

  Faculty Of Bioresources And Center For Molecular Biology And Genetics Mie University

• KIMURA Tetsuya

  Faculty of Bioresources, Mie University

• Kajino T

  Special Research Laboratory Ii Toyota Central R&d Laboratories Inc.

• Aburatani Takeshi

  Faculty Of Bioresources Mie University

• SAKKA Kazuo

  Faculty of Bioresources, Mie University

• KARITA Shuichi

  Center for Molecular Biology and Genetics Mie University

• MAKINO Takashi

  Faculty of Bioresources, Mie University

• Sakka Kazuo

  Faculty Of Bioresources Mie University

• Arai T

  National Inst. Of Fruit Tree Sci. Nagasaki Jpn

• Takahashi Masaaki

  Mie Pref. Institute Of Public Health & Environmental Science

• Kimura Tetsuya

  Graduate School Of Bioresources Mie University

• Kimura Tetsuya

  Faculty Of Bioresources Mie University

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