Purification and Characterization of the Overexpressed Aspergillus oryzae Xylanase, XynF1

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概要

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• The Aspergillus oryzae xynF1 gene coding for a xylanase, XynF1,was successfully overexpressed under the strong A. oryzae TEF1 gene promoter. The high-XynF1-producing transformant secreted about 180mg/l of XynF1 in the glucose-containing medium. The overexpressed XynF1 was purified by only one chromatographic step. The purified XynF1 had a molecular mass of 35.0 kDa, a pH optimum of 5.0,and a temperature optimum of 60℃.

• 社団法人日本農芸化学会の論文
• 1999-10-23

著者

• Ohmiya Kunio

  Faculty Of Bioresources Mie University

• Kitamoto Noriyuki

  Food Research Center, Aichi Industrial Technology Institute

• TSUKAGOSHI Norihiro

  Department of Food Science and Technology, Faculty of Agriculture, Nagoya University

• YOSHINO Shoko

  Food Research Institute, Aichi Prefectural Government

• Yoshino Shoko

  Food Research Institute Aichi Prefectural Government

• Kitamoto N

  Food Research Center Aichi Industrial Technology Institute

• Kitamoto Noriyuki

  Food Research Center Aichi Industrial Technology Institute

• Tsukagoshi N

  Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya

• Tsukagoshi Norihiro

  Department Of Applied Biological Sciences Faculty Of Agriculture Nagoya University

• Kitamoto Noriyuki

  Food Res. Center Aichi Ind. Tech. Inst.

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