Sequence-specific Binding Sites in the Taka-amylase A G2 Promoter for the CreA Repressor Mediating Carbon Catabolite Repression
スポンサーリンク
概要
- 論文の詳細を見る
The N-terminal part of the CreA protein encompassing two zinc fingers was expressed in Escherichia coli as a fusion protein with the maltose binding protein (MalE) of E. coli. Our results show that CreA binds to the promoter of the Taa-G2 gene encoding Taka-amylase A of Aspergillus oryzae. DNase I footprinting experiments showed that CreA bound to three sites with high affinity and to one site with low affinity within the first 401-bp region upstream of the transcription initiation site. All of the sites contained sequences related to the CreA consensus binding site (5'-SYGGRG-3'), and are suggested to participate in repression of the Taa-G2 gene in response to glucose.
- 社団法人日本農芸化学会の論文
- 1996-11-23
著者
-
Kato Masashi
Department Of Bioenvironmental Medicine Graduate School Of Medicine Chiba University
-
Sekine Kotaro
Department Of Applied Biological Sciences Faculty Of Agriculture Nagoya University
-
Tsukagoshi Norihiro
Department Of Applied Biological Sciences Faculty Of Agriculture Nagoya University
-
Kato Masashi
Department Of Applied Biological Chemistry Fuculty Of Agriculture Meijo University
-
Kato Masashi
Department Of Applied Biological Sciences Faculty Of Agriculture Nagoya University
-
Kato Masashi
Department Of Applied Biological Chemistry Faculty Of Agriculture Meijo University
関連論文
- PJ-577 The targeting of cyclophilin D by RNA interference as a novel therapeutic strategy against myocardial ischemia/reperfusion injury(Acute coronary syndrome, basic/clinical(05)(IHD),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Ja
- PDZK1 is a potential target for 1q21-q22 amplification frequently detected in drug resistant ovarian cancer(Oncology 1)
- OJ-185 Effects of Cardioprotective Drugs on Mitochondrial Functions during Myocardial Ischemia/Reperfusion Revealed by Real-time Two-photon Imaging of Perfused Rat Hearts.(Myocardial ischemia / reperfusion, basic / clinical(02)(IHD),Oral Presentation (Jap
- PJ-125 Targeting Cyclophilin D by RNA Interference Inhibits Oxidant-Induced Mitochondrial Death Pathway in Cardiac Myocytes(Myocardial ischemia/reperfusion, basic/clinical-3, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- Changes of sperm functions and epididymal gene expression in the rats treated with sulfasalazine for several days(Development, Reproduction, Proceedings of the 32nd Annual Meeting)
- Psychometric validation of the Japanese version of the International Consultation on Incontinence Questionnaire-Short Form
- Differential Regulation of MMP-9 and TIMP-2 Expression in Malignant Melanoma Developed in Metallothionein/RET Transgenic Mice
- Annealing Study of the Electrochemically Deposited InS_xO_y Thin Film and Its Photovoltaic Application
- Genetic Transformation of Bacillus brevis with Plasmid DNA by Electroporation(Microbiology & Fermentation Industry)
- TRENDS IN RECENT RESEARCH ON POSSIBLE EFFECTS OF ENDOCRINE DISRUPTORS ON HUMAN HEALTH
- Effect of Exposure to High Isoflavone-Containing Diets on Prenatal and Postnatal Offspring Mice
- Deep Level Study in Heteroepitaxial 3C-SiC Grown on Si by Hexamethyldisilane : Electrical Properties of Condensed Matter
- Expression Profile of Amylolytic Genes in Aspergillus nidulans
- The Region in a Subunit of the Aspergillus CCAAT-Binding Protein Similar to the HAP4p-Recruiting Domain of Saccharomyces cerevisiae Hap5p Is Not Essential for Transcriptional Enhancement
- Mode of AmyR Binding to the CGGN_8AGG Sequence in the Aspergillus oryzae taaG2 Promoter
- No Factors Except for the Hap Complex increase the Taka-amylase A Gene Expression by Binding to the CCAAT Sequence in the Promoter Region(Biochemistry & Molecular Biology)
- In Vivo and in Vitro Analyses of the AmyR Binding Site of the Aspergillus nidulans agdA Promoter ; Requirement of the CGG Direct Repeat for Induction and High Affinity Binding of AmyR(Biochemistry & Molecular Biology)
- A Simple and Rapid Method for the Preparation of a Cell-free Extract with CCAAT-Binding Activity from Filamentous Fungi
- Non-neoplastic glandular structures in a benign peripheral nerve sheath tumor
- Passivation of Deep Levels in 3C-SiC on Si by a Hydrogen Plasma Treatment
- Search for Midgap Levels in 3C-SiC Grown on Si Substrates
- Excess Carrier Lifetime Measurement for Plasma-Etched GaN by the Microwave Photoconductivity Decay Method
- Pectin Methylesterase Gene (pmeA) from Aspergillus oryzae KBN616 : Its Sequence Analysis and Overexpression, and Characterization of the Gene Product
- A Second Pectin Lyase Gene (pel2)from Aspergillus oryzae KBN616 : Its Sequence Analysis and Overexpression, and Characterization of the Gene Products
- Sequence Analysis and Overexpression of a Pectin Lyase Gene (pel1) from Aspergillus oryzae KBN616
- Purification and Characterization of the Overexpressed Aspergillus oryzae Xylanase, XynF1
- Analysis by Deletion and Site-directed Mutagenesis of Promoters of the Cell Wall Protein Gene Operon in Bacillus brevis 47(Microbiology & Fermentation Industry)
- Enhancement of Interleukin-la Mediated Autocrine Growth of Cultured Human Keratinocytes by Sho-saiko-to
- The Nitrate Reductase Gene from a Shoyu Koji Mold, Aspergillus oryzae KBN616
- Sacrificial Anodic Oxidation of 6H-SiC : Semiconductors
- Observation of Defects that Reduce Schottky Barrier Height in 4H-SiC Schottky Contacts Using Electrochemical Deposition of ZnO
- Inducer-Dependent Nuclear Localization of a Zn(II)_2Cys_6 Transcriptional Activator, AmyR, in Aspergillus nidulans
- Regulation of the Violacein Biosynthetic Gene Cluster by Acylhomoserine Lactone-Mediated Quorum Sensing in Chromobacterium violaceum ATCC 12472
- Purification and Properties of Divalent Cation-Dependent Raw-Starch-Digesting α-Amylase from Bacillus sp. B1018
- Novel Promoter Sequence Required for Inductive Expression of the Aspergillus nidulans Endoglucanase Gene eglA
- Formation of Superoxide Anion during Ferrous Ion-Induced Decomposition of Linoleic Acid Hydroperoxide under Aerobic Conditions
- Observation of Regions with Low Schottky Barrier Height in 4H-SiC by the Electrochemical Deposition(Session9B: GaN and SiC Device Process Technology)
- Observation of Regions with Low Schottky Barrier Height in 4H-SiC by the Electrochemical Deposition(Session9B: GaN and SiC Device Process Technology)
- Development of a Highly Efficient Gene Replacement System for an Industrial Strain of Aspergillus oryzae Used in the Production of Miso, a Japanese Fermented Soybean Paste
- Mapping of Melanoma Modifier Loci in RET Transgenic Mice
- Secretion of Human Interleukin-2 in Biologically Active Form by Bacillus brevis Directly into Cultute Medium
- BbrPI, an Extracellular Proteinase Inhibitor of Bacillus brevis, Protects Cells from the Attack of Exogenous Proteinase
- Xylose Triggers Reversible Phosphorylation of XlnR, the Fungal Transcriptional Activator of Xylanolytic and Cellulolytic Genes in aspergillus oryzae
- Molecular Role of Ascorbate in Enhancement of NO Production in Activated Macrophage-Like Cell Line, J774.1
- Sequence-specific Binding Sites in the Taka-amylase A G2 Promoter for the CreA Repressor Mediating Carbon Catabolite Repression
- L-Ascorbic Acid 2-Phosphate Promotes Osteoblastic Differentiation of MC3T3-E1 Mediated by Accumulation of Type I Collagen
- Increase in the Activity of Alkaline Phosphatase by L-Ascorbic Acid 2-Phosphate in a Human Osteoblast Cell Line, HuO-3N1
- An Overview of the CCAAT-Box Binding Factor in Filamentous Fungi : Assembly, Nuclear Translocation, and Transcriptional Enhancement
- Observation of Inhomogeneity of Schottky Barrier Height on 4H–SiC Using the Electrochemical Deposition
- Excess Carrier Lifetime in a Bulk p-Type 4H–SiC Wafer Measured by the Microwave Photoconductivity Decay Method
- Excess Carrier Lifetime Measurement of Bulk SiC Wafers and Its Relationship with Structural Defect Distribution
- Periurethral injection of autologous adipose-derived regenerative cells for the treatment of male stress urinary incontinence : Report of three initial cases
- Disruption and Overexpression of Acid Phosphatase Gene (aphA) from a Miso Koji Mold, Aspergillus oryzae KBN630, and Characterization of the Gene Product
- BMP-4 down-regulates the expression of Ret in murine melanocyte precursors
- Sequence Analysis and Heterologous Expression of Polygalacturonase Gene (AspecA) from a Shoyu Koji Mold, Aspergillus sojae KBN1340
- Estimation of Surface Recombination Velocity from Thickness Dependence of Carrier Lifetime in n-Type 4H-SiC Epilayers
- YW2-08 A Follow-up Study of CD 28^-CD4^+T cells In Patients With Long-term Allograft Acceptance (Young Urologist Research Workshop)
- Passivation of Deep Levels in 3C-SiC on Si by a Hydrogen Plasma Treatment
- Leakage Current Suppression by Passivation of Defects by Anodic Oxidation of 4H-SiC Schottky Contacts
- Molecular Analysis of AsamyR Gene Encoding Transcriptional Factor for Amylolytic Gene from Shoyu Koji Mold, Aspergillus sojae KBN1340
- Pretreatment of Renal Supscapular Administration of Adipose Tissue-derived Stem Cells Ameliorate Ischemia-reperfusion-Induced Acute Kidney Injury [Innovation in Transplant and Regenerative Medicine. The 13th Meeting of Hirosaki International Forum of Medi
- Molecular Analysis of the α-Amylase Gene, AstaaG1, from Shoyu Koji Mold, Aspergillus sojae KBN1340
- Detection of Plasmids in Bacillus brevis Species and Introduction of a Plasmid into B. brevis 47, a Protein-producing Bacterium, by Cotransformation
- Sequence Analysis and Heterologous Expression of Rhamnogalacturonan Lyase A Gene (AsrglA) from Shoyu Koji Mold, Aspergillus sojae KBN1340
- Sequence Analysis and Heterologous Expression of Rhamnogalacturonan Lyase A Gene (AsrglA) from Shoyu Koji Mold, Aspergillus sojae KBN1340
- Stability of a recombinant plasmid containing .ALPHA.-amylase gene in chemostat.
- Electrochemical Etching of 6H-SiC Using Aqueous KOH Solutions with Low Surface Roughness
- Clones with New Phenotypes through Transformation of Bacillus brevis 47, a Protein-producing Bacterium
- Excess Carrier Lifetime in p-Type 4H-SiC Epilayers with and without Low-Energy Electron Irradiation
- Molecular Analysis of the α-Amylase Gene, AstaaG1, from Shoyu Koji Mold, Aspergillus sojae KBN1340
- Observation of deep levels and their hole capture behavior in p-type 4H-SiC epilayers with and without electron irradiation