A Second Pectin Lyase Gene (pel2)from Aspergillus oryzae KBN616 : Its Sequence Analysis and Overexpression, and Characterization of the Gene Products
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概要
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A second pectin lyase gene, designated pel2, was isolated from a shoyu koji mold Aspergillus oryzae KBN616 and characterized. The structural gene comprised 1306 bp with three introns. The ORF encoded 375 amino acids with a signal peptide of 19 amino acids. The deduced amino acid sequence showed high similarity to those of A.oryzae Pel1, Aspergillus niger pectin lyases and Glomerella cingulata PnlA. The pel2 gene was overexpressed under the control of the promoter of the A.oryzae TEF1 gene for purification and enzymatic characterization of its gene product. The gene product exhibited two molecular masses of 48 and 44 kDa due to different degrees of glycosylation. Both proteins had the same pH optimum of 6.0 and temperature optimum of 50℃.
- 社団法人日本生物工学会の論文
- 2001-04-25
著者
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Ohmiya Kunio
Faculty Of Bioresources Mie University
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Kitamoto Noriyuki
Food Research Center, Aichi Industrial Technology Institute
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TSUKAGOSHI Norihiro
Department of Food Science and Technology, Faculty of Agriculture, Nagoya University
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Yoshino Shoko
Food Research Institute Aichi Prefectural Government
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YOSHINO-YASUDA SHOKO
Food Research lnstitute, Aichi Prefectural Government
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Kitamoto N
Food Research Center Aichi Industrial Technology Institute
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Kitamoto Noriyuki
Food Research Center Aichi Industrial Technology Institute
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Tsukagoshi N
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
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Tsukagoshi Norihiro
Department Of Applied Biological Sciences Faculty Of Agriculture Nagoya University
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Kitamoto Noriyuki
Food Res. Center Aichi Ind. Tech. Inst.
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YOSHINO-YASUDA Shoko
Food Research Center, Aichi Center for Industry and Science Technology
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