Cloning and Expression of NAD^+-Dependent L-Arabinitol 4-Dehydrogenase Gene (ladA) of Aspergillus oryzae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
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概要
- 論文の詳細を見る
A gene of Aspergillus oryzae, ladA, which encodes L-arabinitol 4-dehydrogenase (EC 1.1.1.12), and its cDNA were cloned in Escherichia coli. The gene consisted of a 1209-bp coding region, interrupted by a 59-bp intron, which encoded a 382-amino-acid polypeptide (40,812 Da). The protein showed 67% identity to a well-studied L-arabinitol 4-dehydrogenase (Lad1) of Hypocrea jecorina. The cell-free extract of E. coli, which expressed ladA cDNA, showed L-arabinitol dehydrogenase activity with NAD^+. It was also reactive for ribitol and xylitol.
- 社団法人日本生物工学会の論文
- 2005-10-25
著者
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鈴木 徹
岐阜大院・連農・生資科
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KAWAI Keiichi
Faculty of Health Science, School of Medicine, Kanazawa University
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SUZUKI Tohru
United Graduate School of Agricultural Science, Gifu University
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Kawai K
Faculty Of Applied Biological Sciences Gifu University
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Suzuki T
Life Science Research Center Gifu University:faculty Of Applied Biological Sciences Gifu University
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Tran Lien
Institute Of Biological Food Technology Hanoi University Of Technology
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Suzuki Tohru
Life Science Research Center, Gifu University
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Yogo Masanori
Faculty of Applied Biological Sciences, Gifu University
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Idota Osamu
Faculty of Applied Biological Sciences, Gifu University
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Kitamoto Noriyuki
Food Research Center, Aichi Industrial Technology Institute
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Takamizawa Kazuhiro
Faculty of Applied Biological Sciences, Gifu University
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Saito T
Kanagawa Univ. Kanagawa Jpn
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Suzuki T
Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University
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Suzuki Tohru
Life Science Research Center Gifu University:faculty Of Applied Biological Sciences Gifu University
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So Tomoko
Second Department Of Surgery Faculty Of Medicine University Of Occupational And Environmental Health
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So Tetsuya
Second Department Of Surgery School Of Medicine University Of Occupational And Environmental Health
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Idota Osamu
Faculty Of Applied Biological Sciences Gifu University
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Kawai Keiichi
Faculty Of Applied Biological Sciences Gifu University
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Takamizawa K
Faculty Of Applied Biological Sciences Gifu University
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Yogo Masanori
Faculty Of Applied Biological Sciences Gifu University
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Kitamoto N
Food Research Center Aichi Industrial Technology Institute
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Kitamoto Noriyuki
Food Research Center Aichi Industrial Technology Institute
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SUZUKI Tohru
Faculty of Applied Biological Sciences, Gifu University
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Suzuki Tohru
The United Graduate School Of Agricultural Science Gifu University
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Suzuki Tohru
Molecular Genetics Research Center Gifu University
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Suzuki Tohru
Life Science Research Center Gifu University
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Suzuki Tohru
United Grad. Sch. Agr. Sci. Gifu Univ.:fac. Appl. Biol. Sci. Gifu Univ.
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Kitamoto Noriyuki
Food Res. Center Aichi Ind. Tech. Inst.
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SUZUKI Tohru
The United Graduate School of Agricultural Science, Gifu University
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Takamizawa Kazuhiro
Faculty of Applied Biological Science, Gifu University
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