Expression of xyrA Gene Encoding for D-Xylose Reductase of Candida tropicalis and Production of Xylitol in Escherichia coli

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概要

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• The D-Xylose reductase (XR) gene (xyrA) of Candida tropicalis IFO 0618 was expressed in Escherichia coli JM109. The enzymatic properties of each recombinant XR such as the K_m value for D-xylose and NADPH, the substrate specificity for other sugars and the optimal pH were essentially the same as those of the corresponding enzyme of C.tropicalis. The recombinant XR was more heat-stable than C.tropicalis XR at 60°C.E.coli, expressing the xyrA gene, successfully converted D-xylose to xylitol. When D-xylose (50g/l) and D-glucose (5g/l) were added to IPTG-induced cells, 13.3g/l of xylitol was produced during 20h of cultivation.

• 社団法人日本生物工学会の論文
• 1999-03-25

著者

• Yokoyama Shin-ichiro

  Department of Medicine, Division of Cardiovascular Medicine, Nihon University School of Medicine

• Yokoyama S

  Laboratory Of Microbiology Department Of Public Health Pharmacy Gifu Pharmaceutical University

• SUZUKI Tohru

  United Graduate School of Agricultural Science, Gifu University

• Yokoyama S

  Takara Shuzo Co. Ltd. Shiga Jpn

• Kawai K

  Faculty Of Applied Biological Sciences Gifu University

• HATSU Masahiro

  Department of Bioprocessing, Faculty of Agriculture, Gifu University

• TAKAMIZAWA Kazuhiro

  Department of Bioprocessing, Faculty of Agriculture, Gifu University

• Suzuki T

  Life Science Research Center Gifu University:faculty Of Applied Biological Sciences Gifu University

• YAMADA Hiroko

  Department of Biotechnology, Tottori University

• SUZUKI TOHRU

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• Kawai Keiichi

  Department of Biotechnology, Division of Bioresources Utilization, Faculty of Agriculture, Gifu Univ

• Takamizawa Kazuhiro

  Faculty of Applied Biological Sciences, Gifu University

• KINOSHITA YOSHIKO

  Department of Biotechnology, Faculty of Agriculture, Gifu University

• Kinoshita Yoshiko

  Department Of Biotechnology Faculty Of Agriculture Gifu University

• Saito T

  Kanagawa Univ. Kanagawa Jpn

• Suzuki T

  Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University

• So Tetsuya

  Second Department Of Surgery School Of Medicine University Of Occupational And Environmental Health

• Kimata Yuki

  (present Address)faculty Of Home Economics Department Of Food And Nurition Chukyo Women's Unive

• Kawai Keiichi

  Faculty Of Applied Biological Sciences Gifu University

• Takamizawa K

  Faculty Of Applied Biological Sciences Gifu University

• Hatsu M

  Gifu Univ. Gifu Jpn

• Hatsu Masahiro

  Department Of Bioprocessing Faculty Of Agriculture Gifu University

• Yokoyama Shin-ichiro

  Department Of Advanced Medicine Nihon University School Of Medicine

• Takamizawa Kazuhiro

  Department Of Agriculture Gifu University

• Kawai Keiichi

  Department Of Bioresources Utilization Faculty Of Agriculture Gifu University

• Yamada Hiroko

  Department Of Biology Faculty Of Education Saitama University:(present Address)shikamachi Junior Hig

• Yamada Hiroko

  Department Of Biotechnology Faculty Of Agriculture Gifu University

• KAWAI Keiichi

  Department of Agricultural Chemistry, Faculty of Agriculture, Gifu University

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