Thermostable Flavin Reductase That Couples with Dibenzothiophene Monooxygenase, from Thermophilic Bacillus sp. DSM411 : Purification, Characterization, and Gene Cloning
スポンサーリンク
概要
- 論文の詳細を見る
Flavin reductase is essential for the oxygenases involved in microbial dibenzothiophene (DBT) desulfurization. An enzyme of the thermophilic strain, Bacillus sp. DSM411, was selected to couple with DBT monooxygenase (DszC) from Rhodococcus erythropolis D-1. The flavin reductase was purified to homogeneity from Bacillus sp. DSM411, and the native enzyme was a monomer of Mr 16 kDa. Although the best substrates were flavin mononucleotide and NADH, the enzyme also used other flavin compounds and acted slightly on nitroaromatic compounds and NADPH. The purified enzyme coupled with DszC and had a ferric reductase activity. Among the flavin reductases so far characterized, the present enzyme is the most thermophilic and thermostable. The gene coded for a protein of 155 amino acids with a calculated mass of 17,325 Da. The enzyme was overproduced in Escherichia coli, and the specific activity in the crude extracts was about 440-fold higher than that of the wild-type strain, Bacillus sp. DSM411.
- 社団法人 日本農芸化学会の論文
- 2004-08-23
著者
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大城 隆
鳥取大・工・生応工
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Izumi Yoshikazu
Dep. Of Biotechnology Tottori Univ.
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Ohshiro Takashi
Department of Biotechnology, Tottori University
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Ishii Yoshitaka
Department of Applied Chemistry, School of Science and Engineering, Waseda University
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IZUMI Yoshikazu
Department of Biotechnology, Tottori University
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Matsubara Toshiyuki
Department of Biotechnology, Tottori University
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YAMADA Hiroko
Department of Biotechnology, Tottori University
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SHIMODA Tomohisa
Department of Biotechnology, Tottori University
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Ishii Yoshitaka
Institute For Biomedical Engineering Consolidated Research Institute For Advanced Science And Medica
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Ohshiro Takashi
Department Of Biotechnology Faculty Of Engineering Tottori University
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Ishii Yoshitaka
Bio-refining Process Laboratory Advanced Technology And Research Institute Petroleum Energy Center
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Izumi Yoshikazu
Department Of Agricultural Chemistry Kyoto University
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大城 隆
Department Of Biotechnology Tottori University
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Matsubara Toshiyuki
Department Of Biotechnology Tottori University
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Shimoda Tomohisa
Department Of Biotechnology Tottori University
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Yamada Hiroko
Department Of Biology Faculty Of Education Saitama University:(present Address)shikamachi Junior Hig
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Yamada Hiroko
Department Of Biotechnology Tottori University
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