Enzymatic Conversion of Dethiobiotin to Biotin in Cell-free Extracts of a Bacillus sphaericus bioB Transformant

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概要

• 論文の詳細を見る

• The activity of biotin synthase, responsible for biotin synthesis from dethiobiotin, was demonstrated in a completely defined reaction mixture with cell-free extracts of a Bacillus sphaericus bioB transformant. Among the sulfur compounds tested, only S-adenosyl-L-methionine was active, while L-methionine and L-cysteine had no significant effect. Protein concentrations higher than 15mg/ml in the reaction mixture were needed to detect biotin synthase activity. When dialyzed cell-free extracts were used for the reaction, NADH, NADPH, or FAD among the well-known cofactors tested enhanced the activity, and Fe^<2+>, Mn^<2+>, and Ca^<2+> among the metal ions tested also had some effects.

• 社団法人日本農芸化学会の論文
• 1994-09-23

著者

• Marquet Andree

  Laboratoire De Chimie Organique Biologique- Ura Cnrs 493- Universite Pierre Et Marie Curie

• Florentin Dominique

  Laboratoire De Chimie Organique Biologique- Ura Cnrs 493- Universite Pierre Et Marie Curie

• Ohshiro Takashi

  Department of Biotechnology, Tottori University

• IZUMI Yoshikazu

  Department of Biotechnology, Tottori University

• YAMAMOTO MANABU

  Department of Gastroenterologic Surgery, National Kyushu Cancer Center

• YAMAMOTO Manabu

  Graduate School of Information Science and Technology, Hokkaido University

• Ohshiro Takashi

  Department Of Biotechnology Faculty Of Engineering Tottori University

• Izumi Y

  Tottori Univ. Tottori Jpn

• Izumi Yoshikazu

  Department Of Agricultural Chemistry Kyoto University

• Bui Tse

  Laboratoire de Chimie Organique Biologique-URA CNRS 493-Universite Pierre et Marie Curie

• Yamamoto Manabu

  Graduate School Of Information Science And Technology Hokkaido University

• Yamamoto Manabu

  Department Of Applied Physics Faculty Of Engineering Tokyo University Of Agriculture And Technology

• Yamamoto Manabu

  Department of Applied Electronics, Tokyo University of Science, 2641 Yamasaki, Noda, Chiba 278-8510, Japan

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