Agar Plate Method Using Lactobacillus plantarum for Biotin Determination in Serum and Urine.
スポンサーリンク
概要
- 論文の詳細を見る
An improved agar plate method of biotin bioassay using Lactobacillus plantarum ATCC 8014 and bromocresol purple was estab-lished to determine biotin levels in human serum and urine. Samples were treated with 4.5 N H2SO4 to liberate free biotin, autoclaved for 1 h and neutralized by 4.5N NaOH, then 10μl was added to wells in each plate. The biotin levels were measured in 190 serum and 59 urine samples, and the means were 2.7±0.53ng/ml and 12.4±5.56ng/mg of creatinine, re-spectively. The intra-assay coefficient varience (CV) were 3.2 (n=20) and 1.3% (n=23), respectively. The recovery of biotin added (10ng/ml) to serum was 110.7%, and to urine was 99.6%. These findings suggest that this assay is sufficiently accurate and reproducible for routine use in the clinical laboratory. The excretion of orally administered biotin was also demonstrated by the method.
- 財団法人 学会誌刊行センターの論文
著者
-
Izumi Yoshikazu
Department Of Agricultural Chemistry Kyoto University
-
FUKUI Toru
Byotai Seiri Laboratory
-
OIZUMI Jun
National Children's Medical Research Center
-
IINUMA Kazuso
National Children's Medical Research Center
関連論文
- Dibenzothiophene Desulfurizing Enzymes from Moderately Thermophilic Bacterium Bacillus subtilis WU-S2B : Purification, Characterization and Overexpression(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Thermostable Flavin Reductase That Couples with Dibenzothiophene Monooxygenase, from Thermophilic Bacillus sp. DSM411 : Purification, Characterization, and Gene Cloning
- Enhancement and Stabilization of Desulfurization Activity of Rhodococcus erythropolis KA2-5-1 by Feeding Ethanol and Sulfur Components(BIOCHEMICAL ENGINEERING)
- Identification of the Gene Encoding a NAD(P)H-Flavin Oxidoreductase Coupling with Dibenzothiophene (DBT)-Desulfurizing Enzymes from the DBT-Nondesulfurizing Bacterium Paenibacillus polymyxa A-1
- Bioconversion of Dethiobiotin into Biotin by Resting Cells and Protoplasts of Bacillus sphaericus bioB Transformant
- Functional Analysis of Two Solanesyl Diphosphate Synthases from Arabidopsis thaliana
- A Novel Enzyme, L-Tryptophan Oxidase, from a Basidiomycete, Coprinus sp. SF-1 : Purificationa and Characterization
- Purification and Characterization of Methanol Dehydrogenase of a Serine-Producing Methylotroph, Hyphomicrobium methylovorum
- Threonine Production from Glycine and Ethanol by a Methanol-Utilizing Bacterium
- Characterization of Biotin Biosynthetic Enzymes of Bacillus sphaericus: a Dethiobiotin producing Bacterium
- Biological Activity of β-Dolabrin, γ-Thujaplicin, and 4-Acetyltropolone, Hinokitiol-Related Compounds(Biochemistry/Molecular Biology)
- Biological Activity of α-Thujaplicin, the Isomer of Hinokitiol(Biochemistry/Molecular Biology)
- An Obligate Methylotrophic Hyphomicrobium Strain Identification, Growth Characteristics and Cell Composition
- L-Serine Production by Resting Cells of a Methanol-Utilizing Bacterium
- CoASAc-independent Phosphoenolpyruvate Carboxylase from an Obligate Methylotroph Hyphomicrobium methylovorum GM2 : Purification and Characterization
- Further Characterization of Serine Hydroxymethyltransferase from a Serine-producing Methylotroph, Hyphomicrobium methylovorum
- Production of Pyranose Oxidase by Basidiomycetous Fungus No. 52
- Purification and Properties of Pyranose Oxidase from Basidiomycetous Fungus No. 52(Microbiology & Fermentation Industry)
- Isolation of a New Pyranose Oxidase Producing Basidiomycete(Microbiology & Fermentation Industry)
- Biotin-Protein Ratios and Stability of Biotinylated Immunoglobulins as Standards for the Quantitation of Biotin-Binding Immunoglobulins(Analytical Biochemistry)
- Detection of Biotin-Binding Immunoglobulin G in Human Sera Using Avidin-Coated Multiwell Microplates
- Quantitation of Biotin-Binding Immunoglobulins G, A, and M in Human Sera Using F(ab')_2Anti-human Immunoglobulin-Coated Microplates(Analytical Biochemistry)
- Adaptation to Stress when Considered in Relation to Food Intake
- Quantitative Measurement of Biotin-Binding Immunoglobulin G in Human Sera Using F(ab')_2anti-human IgG-Coated Multi-well Plates(Analytical Biochemistry)
- Dibenzothiophene (DBT) Degrading Enzyme Responsible for the First Step of DBT Desulfurization by Rhodococcus erythropolils D-1 : Purification and Characterization
- Regulation of Dibenzothiophene Degrading Enzyme Activity of Rhodococcus erythropolis D-1
- Biotin Production by bioB Transformants of a Bacillus sphaericus Mutant Resistant to 1-(2'-Thenoyl)-3,3,3-trifluoroacetone, an Electron Transport Inhibitor
- An Assay for Serine-glyoxylate Aminotransferase(Microbiology & Fermentation Industry)
- Formation of Valine and Leucine by Analog-resistant Mutants of an Obligate Methylotroph, methylomonas aminofaciens
- Amino Acid Formation by Methanol-utilizing Bacteria
- Microbial Desulfurization of Organic Sulfur Compounds in Petroleum
- Stimulatory Factors for Enzymatic Biotin Synthesis from Dethiobiotin in Cell-free Extracts of Escherichia coli
- Enzymatic Conversion of Dethiobiotin to Biotin in Cell-free Extracts of a Bacillus sphaericus bioB Transformant
- Improvement of 2'-Hydroxybiphenyl-2-sulfinate Desulfinase, an Enzyme Involved in the Dibenzothiophene Desulfurization Pathway, from Rhodococcus erythropolis KA2-5-1 by Site-Directed Mutagenesis
- Production of Benzoylformic Acid from Phenylglycine by Saccharomycopsis lipolytica(Microbiology & Fermentation Industry)
- Isolation and Characterization of a Novel Fucoidan-Degrading Microorganism
- Novel Reactivity of Dibenzothiophene Monooxygenase from Bacillus subtilis WU-S2B
- NADH production from NAD^+ using a Formate Dehygdogenase System with Cells of a Methanol-Utilizing Bacterium
- Involvement of Flavin Coenzyme in Dibenzothiophene Degrading Enzyme System from Rhodococcus erythropolis D-1
- Purification and Characterization of Dibenzothiophene (DBT) Sulfone Monooxygenase, an Enzyme Involved in DBT Desulfurization, from Rhodococcus erythropolis D-1
- Characterization of the Biotin Synthase Reaction from Bacillus sphaericus Using the Photoreduced Deazaflavin System
- Characterization of Dibenzothiophene Desulfurization Reaction by Whole Cells of Rhodococcus erythropolis H-2 in the Presence of Hydrocarbon
- 321 NADPH Production Using a Glucose Dehydrogenase System with Intact Cells and Immobilized Cells of Gluconobacter suboxydans
- Efficient L-Serine Production from Methanol and Glycine by Resting Cells of Methylobacterium sp. Strain MN43
- L-Serine Synthesis Using the Resting Hyphomicrobium sp. NCIB10099 Cell under Suppressive Conditions for L-Serine-Degrading Activity
- L-Serine Production Using a Resting Cell System of Hyphomicrobium Strains
- The First Thermophilic α-Oxoamine Synthase Family Enzyme That Has Activities of 2-Amino-3-ketobutyrate CoA Ligase and 7-Keto-8-aminopelargonic Acid Synthase : Cloning and Overexpression of the Gene from an Extreme Thermophile, Thermus thermophilus, and Ch
- Biotin Overproduction by Biotin Analog-resistant Mutants of Bacillus sphaericus
- Enzymatic Assay for Biotin Using Biotinyl-CoA Synthetase
- Agar Plate Method Using Lactobacillus plantarum for Biotin Determination in Serum and Urine.