Purification and Properties of Pyranose Oxidase from Basidiomycetous Fungus No. 52(Microbiology & Fermentation Industry)

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概要

• 論文の詳細を見る

• Pyranose oxidase was purified from the crude extract of basidiomycetous fungus No. 52 about 74-fold to a single protein band on polyacrylamide gel electrophoresis with an overall yield of 23 %. The molecular weight of the native enzyme was about 300,000 on gel filtration and 69,000 on SDS-polyacrylamide gel electrophoresis. Its isoelectric point was pH 6.3. The enzyme activity showed high stability over a wide pH range of 5.5-9.0 and below 50℃, and 60 % of its initial activity remained even after heating at 70℃. The enzyme was inactivated by Ag^+ , Hg^<2+> , and Cu^<2+>. The enzyme contained FAD covalently bound to the protein and was a glycoprotein containing about 0.7% carbohydrate.

• 社団法人日本農芸化学会の論文
• 1990-06-23

著者

• FURUYA Yuji

  Research Laboratory, Ikeda Food Research Co. Ltd.

• Furuya Yuji

  Research Laboratory Ikeda Food Research Co. Ltd.

• Furuya Yuji

  Research Laboratory Ikeda Toka Kogyo Co.

• Furuya Y

  Department Of Biotechnology Faculty Of Engineering Tottori University:research Laboratory Ikeda Food

• YAMADA Hideaki

  Department of Agricultural Chemistry, Kyoto University

• Yamada H

  Department Of Agricultural Chemistry Facutly Of Agriculture Kyoto University

• IZUMI Yoshikazu

  Department of Biotechnology, Tottori University

• Yamada H

  Department Of Biotechnology Faculty Of Engineering Tottori University

• Furuya Yuji

  Department Of Physics Faculty Of Science Hokkaido University

• Yamada H

  Res. Inst. Tuberculosis Tokyo Jpn

• Yamada Hideaki

  Biotechnology Research Center, Toyama Prefectural University

• Furuya Yuji

  Department Of Biotechnology Faculty Of Engineering Tottori University:research Laboratory Ikeda Food

• Yamada H

  Department Of Agricultural Chemistry Kyoto University

• Izumi Y

  Tottori Univ. Tottori Jpn

• Izumi Yoshikazu

  Department Of Agricultural Chemistry Kyoto University

• Yamada Hideaki

  Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University

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