Optimum Culture Conditions for Production by Pseudomonas chlororaphis B23 of Nitrile Hydratase(Microbiology & Fermentaion Industry)
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概要
- 論文の詳細を見る
We sought optimum culture conditions for the production by Pseudomonas chlororaphis B23 of nitrile hydratase activity. Addition of ferric and ferrous ions and the use of methacrylamide as an inducer greatly enhanced nitrile hydratase formation. When P. chlororaphis B23 was cultivated for 26 hr at 25℃ in a medium consisting of 1 g of sucrose, 0.5 g of methacrylamide, 0.2 g of L-cysteine, 0.2g of L-glutamate (Na), 0.2g of L-proline, 50mg of KH_2PO_4, 50mg of K_2HPO_4, 50mg of MgSO_4-7H_2O, and 1 mg of FeSO_4-7H_2O per 100ml of tap water with the pH controlled at pH 7.5 to 7.8, the enzyme activity in the culture broth was 900-times that previously reported.
- 社団法人日本農芸化学会の論文
- 1986-11-23
著者
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Nagasawa Toru
Department of Biomolecular Science, Gifu University
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YAMADA Hideaki
Department of Agricultural Chemistry, Kyoto University
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Nagasawa T
Department Of Agricultural Chemistry Kyoto University
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Nagasawa Toru
Department Of Agricultural Chemistry Kyoto University
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Yamada H
Nagoya Univ. Nagoya Jpn
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Ryuno Koichiro
Nitto Chemical Industry Co. Ltd.
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Watanabe Ichiro
Central Research Laboratory, Nitto Chemical Industry Co., Ltd.
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ENOMOTO Kanehiko
Nitto Chemical Industry Co., Ltd.
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WATANABE Ichiro
Nitto Chemical Industry Co., Ltd.
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Enomoto K
Central Research Laboratory Nitto Chemical Industry Co. Ltd.:(present Office)mitsubishi Rayon Co. Lt
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Watanabe I
T. Hasegawa Co. Ltd. Tokyo Jpn
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Yamada Hideaki
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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