Screening, Isolation and Taxonomical Properties of Microorganisms Having Acrylonitrile-hydrating Activity(Microbiology & Fermentation Industry)

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概要

• 論文の詳細を見る

• Acrylonitrile-hydrating activity was found in various bacteria belonging to the genera Arthrobacter, Corynebacterium, Microbacterium, Nocardia, Pseudomonas and Rhodococcus. A strain, N-774, isolated by acetonitrile enrichment culture from soil showed the highest activity. Taxonomic studies indicated that strain N-774 belonged to the genus Rhodococcus. The acrylonitrile- hydrating enzyme of strain N-774 was constitutively formed in the cells. Besides acrylonitrile, many nitriles were hydrated to the corresponding amides. n-Butyronitrile, succinonitrile and chloroacetonitrille served as suitable substrates. This bacterium could utilize many aliphatic nitriles and amides as a sole source of nitrogen but hardly utilized malononitrile, acrylonitrile or acrylamide. Cells having high nitrile hydratase activity (about 50 units/mg of dry cells) could be easily prepared by cultivation at 30℃ for 40hr in a malt extract and yeast extract medium.

• 社団法人日本農芸化学会の論文
• 1987-12-23

著者

• Satoh Y

  Mitsubishi Petrochemical Co. Ltd. Ibaraki Jpn

• Watanabe Ichiro

  Central Research Laboratory, Nitto Chemical Industry Co., Ltd.

• Enomoto K

  Central Research Laboratory Nitto Chemical Industry Co. Ltd.:(present Office)mitsubishi Rayon Co. Lt

• Watanabe I

  T. Hasegawa Co. Ltd. Tokyo Jpn

• SATOH Yoshiaki

  Central Research Laboratory, Nitto Chemical Industry Co., Ltd.

• ENOMOTO Kanehiko

  Central Research Laboratory, Nitto Chemical Industry Co., Ltd.

• Watanabe Ichiro

  Central Research Laboratories Nitto Chemical Industry Co. Ltd.

関連論文

• Cloning of Two Halohydrin Hydrogen-Halid-Lyase Genes of Corynebacterium sp.Strain N-1074 and Structural Comparison of the Genes and Gene Products
• Microbial Transformation of Prochiral 1,3-Dichloro-2-propanol into Optically Active 3-Chloro-1,2-propanediol(Microbiology & Fermentation Industry)
• Optimum Culture Conditions for Production by Pseudomonas chlororaphis B23 of Nitrile Hydratase(Microbiology & Fermentaion Industry)
• Screening of Microorganisms Producing D-β-Acetylthioisobutyric Acid from Methyl DL-β-Acetylthioisobutyrate
• Optimal Conditions for Cultivation of Rhodococcus sp. N-774 and for Conversion of Acrylonitrile to Acrylamide by Resting Cells(Microbiorogy & Fermentation Industry)
• Screening, Isolation and Taxonomical Properties of Microorganisms Having Acrylonitrile-hydrating Activity(Microbiology & Fermentation Industry)

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