Cloning of Two Halohydrin Hydrogen-Halid-Lyase Genes of Corynebacterium sp.Strain N-1074 and Structural Comparison of the Genes and Gene Products
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概要
- 論文の詳細を見る
We cloned the genes from Corynebacterium sp. strain N-1074, for two halohydrin hydrogen-halide-lyase isoenzymes (hheA and hheB), which are involved in the transformation of alpha, beta-halohydrin into the corresponding epoxide and the reverse reaction. The nucleotide sequences of 1057 base pairs including the hheA gene and of 1130 base pairs including the hheB gene were analyzed. The predicted amino acid sequence of the hheA gene product consisted of 244 residues, and the calculated molecular weight was 26, 465. The hheB gene had two sets of potential ribosome binding site and a possible initiation codon. The predicted amino acid sequences of the gene products consisted of 235 and 227 residues, and the calculated molecular weights were 26, 179 and 25, 236, respectively. Site-directed mutagenesis experiments strongly suggested dual translational initiation in the hheB gene. Comparison of the predicted amino acid sequences for the hheA and hheB genes found signiticant homology only in the carboxyl terminal region. An analysis of the upstream regions of the both hhe genes suggested the presence of putative epoxide hydrolase genes, which might be involved in further degradation of epoxide compounds.
- 社団法人日本農芸化学会の論文
- 1994-08-23
著者
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Nakamura Tomohiko
キリンビール
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Nakamura Tomohiko
Applied Bioresearch Center Corporate Research And Development Division Kirin Brewery Co. Ltd.
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Nakamura Tomohiko
Applied Bioresearch Center Corporate Research And Development Department Kirin Brewery Co. Ltd.
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Watanabe I
Department Of Electrical And Electronic Engineering Faculty Of Engineering Kanazawa University
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Watanabe I
Sankyo Co. Ltd. Tokyo Jpn
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Nakamura T
Research And Education Center For Genetic Information Nara Institute Of Science And Technology
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Yu Fujio
Central Research Laboratory, Nitto Chemical Industry Co., Ltd.
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Nakamura Tetsuji
Central Research Laboratory, Nitto Chemical Industry Co., Ltd.
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Mizunashi Wataru
Central Research Laboratory, Nitto Chemical Industry Co., Ltd.
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Watanabe Ichiro
Central Research Laboratory, Nitto Chemical Industry Co., Ltd.
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Yu Fujio
Central Research Laboratory Nitto Chemical Industry Co. Ltd.
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Mizunashi Wataru
Central Research Laboratory Nitto Chemical Industry Co. Ltd.
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Watanabe Ichiro
Central Research Laboratories Nitto Chemical Industry Co. Ltd.
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