Three Arabidopsis Genes Encoding Proteins with Differential Activities for Cysteine Synthase and β-Cyanoalanine Synthase
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概要
- 論文の詳細を見る
Three cDNA clones encoding putative cysteine synthases(O-acetylserine(thiol)lyase, EC 4.2.99.8)were isolated from Arabidopsis thaliana and designated AtcysC1, AtcysD1 and AtcysD2, respectively. Southern blot analyses suggested that the corresponding genes were present as a single copy, or at most two copies, in the A.thaliana genome. Escherichia coli complementation analyses confirmed that the cDNAs encode cysteine synthase and the corresponding proteins produced in E.coli clearly showed cysteine synthase activity. In addition, AtcysC1 protein showed β-cyanoalanine synthase(EC 4.4.1.9)activity, but the other two did not. Kinetic analysis suggests that AtcysC1 actually functions as β-cyanoalanine synthase rather than cysteine synthase in vivo. The mRNA accumulation of AtcysC1, AtcysD1 and AtcysD2 differed in various organs, but did not change markedly when A.thaliana seedlings were subjected to various stresses, including mutrient deprivation. In vivo targeting experiments indicated that AtcysD1 and AtcysD2 are cytoplasmic isozymes, and AtcysC1 is a mitochondrial isozyme.
- 日本植物生理学会の論文
著者
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Nakamura Tomohiko
キリンビール
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Sano Hiroshi
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Nakamura Tomohiko
Applied Bioresearch Center Corporate Research And Development Division Kirin Brewery Co. Ltd.
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YAMAGUCHI Yube
Research and Education Center for Genetic Information, Nara Institute of Science and Technology
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NAKAMURA Tatsuo
Research and Education Center for Genetic Information, Nara Institute of Science and Technology
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SANO Hiroshi
Research and Education Center for Genetic Information, Nara Institute of Science and Technology
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KUSANO Tomonobu
Research and Education Center for Genetic Information, Nara Institute of Science and Technology
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Nakamura Tomohiko
Applied Bioresearch Center Corporate Research And Development Department Kirin Brewery Co. Ltd.
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Sano Hiroshi
Research And Education Center For Genetic Information Nara Institute Of Science And Technology
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Yamaguchi Y
Department Of Bioresources Science Graduate School Of Science And Technology Chiba University
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Sano H
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Yamaguchi Yoshitaka
Department Of Biotechnology Faculty Of Engineering Fukuyama University
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Kusano T
Research And Education Center For Genetic Information Nara Institute Of Science And Technology
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Kusano Tomonobu
Research And Education Center For Genetic Information
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Yamaguchi Y
Research And Education Center For Genetic Information Nara Institute Of Science And Technology
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Sano Hiromasa
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Nakamura T
Research And Education Center For Genetic Information Nara Institute Of Science And Technology
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Yamaguchi Yube
Research And Education Center For Genetic Information Nara Institute Of Science And Technology
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Sano Hiroshi
Res. And Edu. Ctr. For Genetic Information Nara Institute Of Science And Technology
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