Introduction of Foreign Genes into Tomato Protoplasts by Electroporation

スポンサーリンク

概要

• 論文の詳細を見る

• The conditions required for the electroporation of a plasmid which harbored the gene for chloramphenicol acetyltransferase (CAT) into protoplasts from cultivated tomato leaves were optimized at 666 or 7,000 V/cm (single discharge) using a 47-μF capacitor in the presence of 10-100μg DNA. The CAT gene was strongly expressed by when under the control of the promoter for the 35S RNA from cauliflower mosaic virus and CAT activity was detected in cells 10 days after electroporation.

• 日本植物生理学会の論文

著者

• Kusano T

  Research And Education Center For Genetic Information Nara Institute Of Science And Technology

• Kusano Tomonobu

  Plant Cenetic Engineering Laboratory Biotechnology Institute Akita Prefectural Cllege Of Agriculture

• Tsukada Mikako

  Plant Cenetic Engineering Laboratory Biotechnology Institute Akita Prefectural Cllege Of Agriculture

• Kitagawa Y

  Akita Prefectural Coll. Agriculture Akita Jpn

• Kitagawa Yoshichika

  Plant Cenetic Engineering Laboratory Biotechnology Institute

関連論文

• Three Arabidopsis Genes Encoding Proteins with Differential Activities for Cysteine Synthase and β-Cyanoalanine Synthase
• ISOLATION AND FUNCTIONAL ANALYSIS OF PHODANESE cDNAs FROM Arabidopsis thaliana
• A Set of Wound Responding Genes in Tobacco Plants
• Alteration in Gene Expression during Cold Treatment of Rice Plant
• DIURNAL VARIATION IN THE CHILLING SENSITIVITY OF CROP SEEDLINGS
• Introduction of Foreign Genes into Tomato Protoplasts by Electroporation

スポンサーリンク