Purification and Properties of Two Deacetylases Produced by Vibrio alginolyticus H-8

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概要

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• The Chitinase-producing bacterium Vibrio alginolyticus H-8 isolated from mud of Hamana Lake also produced two deacetylases for (GlcNAc)_2 extracellularly. Deacetylases DA1 and DA2 were purified from crude enzyme by column chromatography on Q-Sepharose FF, Phenyl Sepharose HP, Gigapite, and Superdex 200 HR. The final preparation was homogeneous in SDS-PAGE. The molecular weights were 48,000 and 46,000 for deacetylases DA1 and DA2, respectively. The pIs, optimum pHs, and optimum temperatures for deacetylases DA1 and DA2 were as follows; DA1, pI 3.3, optimum pH 8.5-9.0, optimum temperature 45℃, DA2, pI 3.5, optimum pH 8.0-8.5, optimum temperature 40℃. Both deacetylases were stable at pHs between 7.0 and 11.0 and at temperatures below 40℃. The activities of both enzymes were inhibited by Ag^+ and Hg-<2+>. ^1H-NMR of the reaction product by deacetylase DA1 for (GlcNAc)_2 showed that the purified deacetylase selectively hydrolyzed the 2-acetamide group at the reducing end of (GlcNAc)_2.

• 社団法人日本農芸化学会の論文
• 1997-07-23

著者

• Sano H

  Marine Biotechnology Inst. Co. Ltd. Shizuoka Jpn

• Sano Hiroshi

  Research And Education Center For Genetic Information Nara Institute Of Science And Technology

• ADACHI Kyoko

  Marine Biotechnology Institute

• MIWA TAN

  K・I Chemical Industry Co. Ltd.,

• Sano Hiroshi

  Marine Biotechnology Institute Shimizu Laboratories

• Yamagishi Masaaki

  Numazu Industrial Research Institute Of Shizuoka Prefecture

• Ono T

  Shigei Medical Research Inst. Okayama

• Ohta T

  Numazu Industrial Res. Inst. Shizuoka Prefecture Numazu‐shi Jpn

• OHISHI KAZUO

  Numazu Industrial Research Institute of Shizuoka Prefecture

• OHTA TOSHIYA

  Numazu Industrial Research Institute of Shizuoka Prefecture

• Miwa T

  K・i Chemical Industry Co. Ltd.

• Izumida H

  Kansai Paint Co. Ltd. Technical Research Laboratory

• Izumida Hitoshi

  The Fuel Alcohol Research Association (fara) Technical Research Laboratory Kansai Paint Co. Ltd.

• Izumida Hitoshi

  Marine Biotechnology Institute Shimizu Laboratories.

• MOTOSUGI Masayoshi

  Numazu Industrial Research Institute of Shizuoka Prefecture

• Ohishi K

  United Graduate School Of Agricultural Sciences Gifu University

• Yamagishi M

  Tachikawa Hospital Tachikawa Jpn

• Adachi Kyoko

  Marine Biotech. Inst.

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