Screening of Wound-Responsive Genes Identifies an Immediate-Early Expressed Gene Encoding a Highly Charged Protein in Mechanically Wounded Tobacco Plants
スポンサーリンク
概要
- 論文の詳細を見る
In order to identify genes that are temporally and spatially regulated during wound response, a cDNA population in mechanically wounded tobacco leaves was screened by the fluorescence differential display method. Of 28 clones initially identified to have altered levels of transcripts within 3h of wounding, eight were characterized. Although each clone showed a unique pattern of transcript accumulation, one distinct clone was further characterized because of its immediate-early response. Its transcripts began to accumulate 10 min after wounding, reached a maximum level within 1h and disappeared after 2h. The response, which occurred repeatably and systemically, was observed by the treatment with propionic acid or erythrosin B, indicating that cytosolic acidification could be one of the signals for immediate-early response of this gene. The cDNA encodes a polypeptide of 513 amino acids with a relative molecular mass of 60,952. The putative polypeptide is rich in lysine(K), glutamic acid(E)and aspartic acid(D), which constitute up to 70% of total amino acids, and was therefore designated as KED. The KED polypeptide is composed of a highly hydrophilic N-terminal region and a relatively hydrophobic C-terminal region, suggesting that KED may function through electrostatic interactions with cellular components.
- 日本植物生理学会の論文
著者
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Sano Hiroshi
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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SANO Hiroshi
Research and Education Center for Genetic Information, Nara Institute of Science and Technology
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KOIZUMI Nozomu
Research and Education Center for Genetic Information, Nara Institute of Science and Technology
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KUSANO Tomonobu
Research and Education Center for Genetic Information, Nara Institute of Science and Technology
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Hara Kojiro
Research and Education Center for Genetic Information, Nara Institute of Science and Technology
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Yagi Mitsue
Research and Education Center for Genetic Information, Nara Institute of Science and Technology
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Koizumi N
Kyoto Univ. Kyoto Jpn
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Sano H
Marine Biotechnology Inst. Co. Ltd. Shizuoka Jpn
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Sano Hiroshi
Research And Education Center For Genetic Information Nara Institute Of Science And Technology
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Koizumi Nozomu
Research And Education Center For Genetic Information
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Sano H
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Koizumi N
Nara Inst. Sci. And Technol. Ikoma Jpn
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Koizumi Nozomu
Plant Molecular Breeding Genetics Center Nara Inst.sci.tech.
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Kusano Tomonobu
Research And Education Center For Genetic Information Nara Institute Of Science And Technology
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Kusano Tomonobu
Research And Education Center For Genetic Information
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Sano Hiromasa
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Kusano Tomonobu
Genetic Research Center Nara Institute Of Science And Technology
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Kusano Tomonobu
Res. And Edu. Ctr. For Genetic Information Nara Institute Of Science And Technology
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Hara Kojiro
Research And Education Center For Genetic Information Nara Inst. Sci. And Tech.
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Yagi Mitsue
Research And Education Center For Genetic Information Nara Inst. Sci. And Tech.
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Koizumi N
Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University
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Sano Hiroshi
Res. And Edu. Ctr. For Genetic Information Nara Institute Of Science And Technology
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