Bacterial Production and Purification of Phosphorylatable Phosphoenolpyruvate Carboxylase from Tobacco
スポンサーリンク
概要
- 論文の詳細を見る
Tobacco phosphoenolpyruvate carboxylase (PEPC) [EC 4.1.1.31] cDNA was efficiently expressed in E. coli under the control of the lacZ promoter. The enzyme, purified to homogeneity, had the same catalytic activity, and was phosphorylated in vitro by maize PEPC kinase.
- 社団法人日本農芸化学会の論文
- 1996-12-23
著者
-
Koizumi N
Kyoto Univ. Kyoto Jpn
-
Sato Fumihiko
Department of Medicine, Metabolism and Endocrinology, Juntendo University School of Medicine
-
Yamada Y
The Department Of Mechanical Engineering And Intelligent Systems University Of Electro-communication
-
Sato Fumihiko
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
-
KOIZUMI Nozomu
Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University
-
YAMADA Yasuyuki
The Graduate School of Biological Science, Nara Institute of Science and Technology
-
Yamada Yukio
Fine Chemicals Research Laboratories Kaneka Corporation
-
Koizumi N
Nara Inst. Sci. And Technol. Ikoma Jpn
-
Koizumi Nozomu
Plant Molecular Breeding Genetics Center Nara Inst.sci.tech.
-
Koizumi Nozomu
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University:research And Education
-
Yamada Yukio
Mechanical Engineering Laboratory Material Science And Bioengineering Department Aist
-
Yamada Y
Fac. Of Education Utsunomiya Univ.
-
Koizumi N
Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University
-
Sato Fumihiko
Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University
関連論文
- Combinatorial Microarray Analysis Revealing Arabidopsis Genes Implicated in Cytokinin Responses through the His→Asp Phosphorelay Circuitry
- Rapid Response of Arabidopsis T87 Cultured Cells to Cytokinin through His-to-Asp Phosphorelay Signal Transduction
- Isolation and Characterization of a Putative Transducer of Endoplasmic Reticulum Stress in Oryza sativa
- IDENTIFICATION OF EARLY EXPRESSED GENES IN TOBACCO PLANTS DURING HYPERSENSITIVE RESPONSE UPON TMV INFECTION
- PROMOTER ANALYSIS OF WIPK-A TOBACCO WOUND INDUCED MAP KINASE
- An Arabidopsis SNF1-related protein kinase, AtSR1,interacts with a calcium-binding protein, CBL2
- Cytosine methylation of chloroplast DNA in Chlamydomonas reinhardtii
- Screening of Wound-Responsive Genes Identifies an Immediate-Early Expressed Gene Encoding a Highly Charged Protein in Mechanically Wounded Tobacco Plants
- Differential Accumulation of Transcripts Encoding Sulfur Assimilation Enzymes upon Sulfur and/or Nitrogen Deprivation in Arabidopsis thaliana
- Molecular characterization of a gene encoding an Ire1 homolog from rice
- A Set of Wound Responding Genes in Tobacco Plants
- IDENTIFICATION AND CHARACTERIZATION OF SECRETED PROTEINS FROM CULTURED TOBACCO CELLS
- SECRETED PROTEINS FROM CULTURED TOBACCO CELLS
- Intracellular lipid accumulation and insulin resistance in skeletal muscle and liver
- Therapeutic Efficacy of Mitiglinide Combined with Once Daily Insulin Glargine after Switching from Multiple Daily Insulin Regimen of Aspart Insulin and Glargine in Patients with Type 2 Diabetes Mellitus
- Fabrication of Realistic and Dynamic Human Head Phantoms
- Isolation of a Protease-Deficient Mutant of Bacillus brevis and Efficient Secretion of a Fungal Protein Disulfied Isomerase by the Mutant
- Simple and Rapid Screening Method for Photosystem II Inhibitory Herbicides Using Photoautotrophically Cultured Plant Cells with Chlorophyll Fluorescence Monitoring
- A Potent Hypotensive Peptide, Novokinin, Induces Relaxation by AT2- and IP-Receptor-Dependent Mechanism in the Mesenteric Artery from SHRs
- Design of a Highly Potent Anti-hypertensive Peptide Based on Ovokinin(2-7)(Biochemistry & Molecular Biology)
- Designing Potent Derivatives of Ovokinin(2-7), an Anti-hypertensive Peptide Derived from Ovalbumin
- Variable Interactions between Sucrose Non-fermented 1-Related Protein Kinases and Regulatory Proteins in Higher Plants(Biochemistry & Molecular Biology)
- An Arabidopsis SNF1-Related Protein Kinase, AtSR1, Interacts with a Calcium-Binding Protein, AtCBL2, of Which Transcripts Respond to Light
- EARLY RESPONDING GENES TO WOUND STRESS OF TOBACCO PLANTS
- Application of the Freeze-Blast Method to Disruption of Cultured Plant Cells
- Bacterial Production and Purification of Phosphorylatable Phosphoenolpyruvate Carboxylase from Tobacco
- Purification and Characterization of Phosphoenolpyruvate Carboxylase of Photomixotrophically Cultured Green Tobacco Cells
- REGULATION BY NUTRIENTS OF GENES INVOLVED INTHE SULFUR ASSIMILATION PATHWAY OF Arabidopsis thaliana
- Production of Thermotolerant N-Carbamyl-D-Amino Acid Amidohydrolase by Recombinant Escherichia coli
- Thermostability Reinforcement through a Combination of Thermostability-related Mutations of N-Carbamyl-D-Amino Acid Amidohydrolase
- Immobilization of N-Carbamyl-D-Amino Acid Amidohydrolase
- Relationship Between an Increase in Thermostability and Amino Acid Substitutions in N-Carbamyl-D-Amino Acid Amidohydrolase
- Increase in Thermostability of N-Carbamyl-D-Amino Acid Amidohydrolase on Amino Acid Substitutions
- Screening, Characterization, and Cloning of the Gene for N-Carbamyl-D-Amino acid Amidohydrolase from Thermotolerant Soil Bacteria
- Isolation of Agrobacterium sp. Strain KNK712 That Produces N-Carbamyl-D-Amino Acid Amidohydrolase, Cloning of the Gene for this Enzyme, and Properties of the Enzyme
- Strict Glycemic Control Ameliorates the Increase of Carotid IMT in Patients with Type 2 Diabetes
- Molecular Cloning of a Fungal cDNA Encoding Protein Disulfide Isomerase
- Purification of Protein Disulfide Isomerase from a Thermophilic Fungus
- Development of a Simple Non-Freezing Method to Preserve Cultured Plant Cells
- Pigment Synthesis by Immobilized Cultured Cells of Lavandula vera and Characterization of a Component of the Pigments(Microbiology & Fermentation Industry)
- Influence of Carbon Source on Pigment Production by Immobilized Cultured Cells of Lavandula vera
- Immobilized Plant Cell Reactor for Continuous Production of Blue Pigments(Microbiology & Fermentation Industry)
- Enhancement of Pigment Productivity of Immobilized Cultured Lavandula vera Cells by Limitation of Nitrogen Sources
- Phylogenetic Relationships of Species of the Genus Saccharomyces MEYEN ex REESS Deduced from Partial Base Sequences of 18S and 26S Ribosomal RNAs (Saccharomycetaceae)
- Phylogenetic Relationships of Species of the Genus Kluyveromyces VAN DER WALT (Saccharomycetaceae) Deduced from the Partial Sequences of 18S and 26S Ribosomal RNAs
- Molecular Cloning and Characterization of S-Adenosyl-L-Methionine : Scoulerine-9-O-Methyltransferase from Cultured Cells of Coptis japonica : GENE STRUCTURE AND EXPRESSION : PROTEINS, ENZYMES AND METABOLISM
- Analysis of Nucleoid-Proteins in Tobacco Chloroplasts
- Photoautotrophic and Photomixotrophic Culture of Green Tobacco Cells in a Jar-Fermenter
- An efficient method of selecting photoautotropbic cells from cultured heterogeneous cells
- 葉緑体NAD(P)Hデヒドロゲナーゼ複合体変異株での電子伝達活性
- Genomic Structure and Promoter Analysis of Phosphoenolpyruvate Carboxylase in a C3 Plant, Nicotiana sylvestris(Biochemistry & Molecular Biology)
- Insight into the Molecular Evolution of Two Tropinone Reductases
- Structures and Expression Patterns of Two Tropinone Reductase Genes from Hyoscyamus niger
- Purification and Characterization of Tobacco Pathogenesis-Related Protein PR-5d, an Antifungal Thaumatin-like Protein
- Spasmolytic Activity of Aurapten Analogs
- The Phylogeny of Species of the Genus Issatchehkia KUDRIAVZEZV (Saccharomycetaceae) Based on the Partial Sequences of 18S and 26S Ribosomal RNAs
- Effects of Oxygen on Nicotine and Tropane Alkaloid Production in Cultured Roots of Duboisia myoporoides
- Scopolamine Yield in Cultured Roots of Duboisia myoporoides Improved by a Novel Two-stage Culture Method
- Increase of Scopolamine Production by High Density Culture of Duboisia myoporoides Roots
- Tropane Alkaloid Production in Root Cultures of Duboisia myoporoides Obtained by Repeated Selection
- Time-Resolved Measurements of in vivo Optical Properties of Piglet Brain
- Anatomical and Functional Images of in vitro and in vivo Tissues by NIR Time-domain Diffuse Optical Tomography(Reviewed Papers Accepted for Publication in this Special Issue)(Special Issue on Bioengineering)
- Time-Resolved Diffuse Optical Tomography Using a Modified Generalized Pulse Spectrum Technique(Special Issue on Measurements and Visualization Technology of Biological Information)
- Transgenic Tobacco Plants with Two Consecutive Oxidation Reactions Catalyzed by Hyoscyamine 6β-Hydroxylase
- Functional Similarities of Recombinant OLP and Cytokinin-Binding Protein 2(Biochemistry & Molecular Biology)
- Ethylene-Induced Gene Expression of Osmotin-Like Protein, a Neutral Isoform of Tobacco PR-5, is Mediated by the AGCCGCC cis-Sequence
- Characterization of Accumulation of Tobacco PR-5 Proteins by IEF-Immunoblot Analysis : ENVIRONMENTAL AND STRESS RESPONSES : PROTEINS, ENZYMES AND METABOLISM
- A Small Subfamily of Arabidopsis RADIALIS-LIKE SANT/MYB Genes : A Link to HOOKLESS1-Mediated Signal Transduction during Early Morphogenesis
- A Link between Cytokinin and ASL9 (ASYMMETRIC LEAVES 2 LIKE 9) That Belongs to the AS2/LOB (LATERAL ORGAN BOUNDARIES) Family Genes in Arabidopsis thaliana
- The Phylogeny of Williopsis salicorniae HINZELIN, KURTZMAN et SMITH Based on the Partial Sequences of 18S and 26S Ribosomal RNAs (Saccharomycetaceae)
- Accumulation of the Bioactive Peptides, Novokinin, LPYPR and Rubiscolin, in Seeds of Genetically Modified Soybean
- A Potent Hypotensive Peptide, Novokinin, Induces Relaxation by AT_2- and IP-Receptor-Dependent Mechanism in the Mesenteric Artery from SHRs
- Characterization of a Unique GATA Family Gene That Responds to Both Light and Cytokinin in Arabidopsis thaliana
- Isolation of Putative Glycoprotein Gene from Early Somatic Embryo of Carrot and its Possible Involvement in Somatic Embryo Development
- CONCENTRATION OF LEVOFLOXACIN IN CERVICAL MUCUS AND ITS CLINICAL EFFECTS ON CERVICITIS
- Photosynthetic carbon metabolism in photoautotrophically and photomixotrophically cultured tobacco cells
- Photosynthetic Characteristics and Carbonic Anhydrase Activity in Cells Cultured Photoautotrophically and Mixotrophically and Cells Isolated from Leaves
- Cloning and Nucleotide Sequence of ApaLI Restriction-modification System from Acetobacter pasteurianus IFO 13753
- Cloning and Nucleotide Sequence of the AgeI Methylase Gene from Agrobacterium gelatinovorum IAM 12617, a Marine Bacterium
- Soymorphin-5, a soy-derived μ opioid peptide, improves glucose and lipid metabolism in diabetic KK-Ay mice
- The Phylogeny of Species of the Genus Saccharomycopsis SCHIONNING (Saccharomycetaceae) Based on the Partial Sequences of 18S and 26S Ribosomal RNAs
- Should Petasospora BOIDIN et ABADIE (Saccharomycetaceae) Be Retained? : The Phylogeny Based on the Partial Sequences of 18S and 26S Ribosomal RNAs
- The Phylogeny of Yamadazyma ohmeri (ETCHELLS et BELL) BILLON-GRAND Based on the Partial Sequences of 18S and 26S Ribosomal RNAs : The Proposal of Kodamaea Gen. Nov. (Saccharomycetaceae)
- The Phylogenetic Relationships of Pichia jadinii, Formerly Classified in the Genus Hansenula, and Related Species Based on the Partial Sequences of 18S and 26S Ribosomal RNAs (Saccharomycetaceae)
- The Phylogenetic Relationships of the Q_9-equipped, Hat-shaped Ascospore-forming Species of the Genus Yamadazyma BILLON-GRAND (Saccharomycetaceae) Based on the Partial Sequences of 18S and 26S Ribosomal RNAs
- The Phylogenetic Relationships of Methanol-assimilating Yeasts Based on the Partial Sequences of 18S and 26S Ribosomal RNAs : The Proposal of Komagataella Gen. Nov.(Saccharomycetaceae)
- The Phylogenetic Relationships of Species of the Genus Dekkera VAN DER WALT Based on the Partial Sequences of 18S and 26S Ribosomal RNAs (Saccharomycetaceae)
- Microbial Transformation of (±)-Epoxyaurpten by Pseudomonas aeruginosa
- Adopting Due-Date Buffer for Real-Time Scheduling Using Lagrangean Relaxation Method in an APS Environment
- 6B2 ADOPTING DUE-DATE BUFFER FOR REAL-TIME SCHEDULING USING LAGRANGEAN RELAXATION METHOD IN AN APS ENVIRONMENT(Technical session 6B : Lagrangian decomposition)
- Photoautotrophy and the photosynthetic potential of chlorophyllous cells in mixotrophic cultures
- Changes Related to Salt Tolerance in Thylakoid Membranes of Photoautotrophically Cultured Green Tobacco Cells : STRUCTURE AND FUNCTION OF CELLS
- The Phylogenetic Relationships of the Saturn-shaped Ascospore-forming Species of the Genus Williopsis Zender and Related Genera Based on the Partial Sequences of 18S and 26S Ribosomal RNAs (Saccharomycetaceae) : The Proposal of Komagataea Gen.Nov.
- The Phylogenetic Relationships of the Hat-shaped Ascospore-forming, Nitrate-assimilat-ing Pichia Species, Formerly Classified in the Genus Hansenula SYDOW et SYDOW,Based on the Partial Sequences of 18S and 26S Ribosomal RNAs (Saccharomycetaceae) : The Pro
- The Phylogenetic Relationships of Rhodosporidium dacryoidum FELL, HUNTER et TALLMAN Based on the Partial Sequences of 18S and 26S Ribosomal RNAs : The Proposal of Sakaguchia Gen. Nov., a Heterobasidiomycetous Yeast Genus
- The photoautotrophic culture of chlorophyllous cells
- An in Vitro Analysis System Using a Fluorescence Protein Reporter for Evaluating Anti-Inflammatory Effects in Macrophages
- A case of long-standing autoimmune type 1 diabetes with common variable immunodeficiency
- An Improved Medium for the Photoautotrophic Culture of Cytisus scoparius Link Cells
- Characterization of chromosomal aberrations in thymic MALT lymphoma