Purification of Protein Disulfide Isomerase from a Thermophilic Fungus
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概要
- 論文の詳細を見る
A protein disulfide isomerase (PDI) was purified to homogeneity from the thermophilic fungus Humicola insolens by a rapid three-step procedure, anion-exchange chromatography, concanavalin A-affinity chromatography, and reverse phase high performance liquid chromatography. Forty-oneμg of PDI was obtained from 100g of wet mycelium. Concanavalin A-Sepharose chromatography is available for purification of the fungal PDI, indicating that the enzyme is also glycosylated like the yeast PDI. The fungal PDI exists as a dimer (2 x 60 kDa), has a pI of 3.5, and is fairly heat-stable. The amino acid composition of the PDI is similar to those of yeast and bovine liver PDI, and the high content of acidic amino acid residues agrees with the lower acidic pI.
- 社団法人日本農芸化学会の論文
- 1993-10-23
著者
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Asami Osamu
Toyota Central Research & Development Labs
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Kajino Tsutomu
Toyota Central Research & Development Labs
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Asami O
Toyota Central Res. & Dev. Labs. Inc. Aichi Jpn
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Kajino T
Toyota Central Research & Development Labs. Inc.
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Kajino Tsutomu
Toyota Central R & D Labs Inc.
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Hoshino Fumihiko
Toyota Central R&D Labs., INC
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YAMADA YUKIO
Toyota Central Research & Development Labs., Inc.
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UDAKA SHIGEZO
Department of Fermentation Sciences, Tokyo University of Agriculture
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Yamada Yukio
Fine Chemicals Research Laboratories Kaneka Corporation
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Idekoba Chie
Toyota Central Research and Development Laboratories Inc.
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Sugiyama Hidehiko
Toyota Central Research & Development Laboratories Inc.
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Hoshino Fumihiko
Toyota Central R&d Labs. Inc
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Hoshino Fumihiko
Toyota Central Research & Development Laboratories Inc.
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Yamada Yukio
Mechanical Engineering Laboratory Material Science And Bioengineering Department Aist
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Udaka S
Faculty Of Applied Bio-science Tokyo University Of Agriculture
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Udaka Shigezo
Department Of Brewing And Fermentation Tokyo University Of Agriculture
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Udaka Shigezo
Dept. Of Food Science And Technology Faculty Of Agriculture Nagoya University
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Sugiyama Hidehiko
Toyota Central Research & Development Laboratories Inc.
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