Purification of Protein Disulfide Isomerase from a Thermophilic Fungus

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概要

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• A protein disulfide isomerase (PDI) was purified to homogeneity from the thermophilic fungus Humicola insolens by a rapid three-step procedure, anion-exchange chromatography, concanavalin A-affinity chromatography, and reverse phase high performance liquid chromatography. Forty-oneμg of PDI was obtained from 100g of wet mycelium. Concanavalin A-Sepharose chromatography is available for purification of the fungal PDI, indicating that the enzyme is also glycosylated like the yeast PDI. The fungal PDI exists as a dimer (2 x 60 kDa), has a pI of 3.5, and is fairly heat-stable. The amino acid composition of the PDI is similar to those of yeast and bovine liver PDI, and the high content of acidic amino acid residues agrees with the lower acidic pI.

• 社団法人日本農芸化学会の論文
• 1993-10-23

著者

• Asami Osamu

  Toyota Central Research & Development Labs

• Kajino Tsutomu

  Toyota Central Research & Development Labs

• Asami O

  Toyota Central Res. & Dev. Labs. Inc. Aichi Jpn

• Kajino T

  Toyota Central Research & Development Labs. Inc.

• Kajino Tsutomu

  Toyota Central R & D Labs Inc.

• Hoshino Fumihiko

  Toyota Central R&D Labs., INC

• YAMADA YUKIO

  Toyota Central Research & Development Labs., Inc.

• UDAKA SHIGEZO

  Department of Fermentation Sciences, Tokyo University of Agriculture

• Yamada Yukio

  Fine Chemicals Research Laboratories Kaneka Corporation

• Idekoba Chie

  Toyota Central Research and Development Laboratories Inc.

• Sugiyama Hidehiko

  Toyota Central Research & Development Laboratories Inc.

• Hoshino Fumihiko

  Toyota Central R&d Labs. Inc

• Hoshino Fumihiko

  Toyota Central Research & Development Laboratories Inc.

• Yamada Yukio

  Mechanical Engineering Laboratory Material Science And Bioengineering Department Aist

• Udaka S

  Faculty Of Applied Bio-science Tokyo University Of Agriculture

• Udaka Shigezo

  Department Of Brewing And Fermentation Tokyo University Of Agriculture

• Udaka Shigezo

  Dept. Of Food Science And Technology Faculty Of Agriculture Nagoya University

• Sugiyama Hidehiko

  Toyota Central Research & Development Laboratories Inc.

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