Structures and Expression Patterns of Two Tropinone Reductase Genes from Hyoscyamus niger

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概要

• 論文の詳細を見る

• In the biosynthesis of tropane alkaloids, two tropinone reductases (TRs) catalyze reduction of tropinone to different stereoisomers, tropine and pseudotropine. Two TRs from Hyoscyamus niger have 64% of identical amino acids and hence a common evolutionary origin. In this study, genomic clones of TRs were isolated from H. niger. Their sequence comparison showed that although they have the same exon/intron organization, sequence similarity was restricted to the coding regions. In H. niger transgenic hairy roots, both TR promoters activated transcription of the reporter genes in endodermis and pericycle of the roots. A quantitative reporter assay and a nuclear run-on experiment indicated that the two genes are transcribed at a similar rate. The results indicate that although different activity levels have been observed for the TR enzymes in the H. niger root, the TR genes per se conserve similar tissuespecific expression pattern and transcriptional rate.

• 社団法人日本農芸化学会の論文
• 1999-10-23

著者

• Hashimoto Takashi

  Graduate School of Biological Sciences, Nara Institute of Science and Technology

• Yamada Y

  Doshisha Women's Coll. Liberal Arts Kyoto Jpn

• Yamada Y

  The Department Of Mechanical Engineering And Intelligent Systems University Of Electro-communication

• Hashimoto T

  Dep. Of Agrobioscience Graduate School Of Agricultural Sci. Kobe Univ.

• Yamada Yukio

  Fine Chemicals Research Laboratories Kaneka Corporation

• Yamada Yasuyuki

  Graduate School Of Biological Science Nara Institute Of Science And Technoiogy

• Hashimoto Takashi

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology

• Nakajima Keiichi

  Research Center For Glycoscience Advanced Institute Of Industrial Science And Technology (aist)

• Nakajima Keiichi

  United Graduate School Of Agricultural Science Tokyo University Of Agriculture And Technology

• Yamada Yukio

  Mechanical Engineering Laboratory Material Science And Bioengineering Department Aist

• Yamada Y

  Fac. Of Education Utsunomiya Univ.

• Yamada Y

  Faculty Of Education Utsunomiya University

• NAKAJIMA Keiji

  Graduate School of Biological Sciences, Nara Institute of Science and Technology

• OSHITA Yoko

  Graduate School of Biological Sciences, Nara Institute of Science and Technology

• KAYA Masahiko

  Graduate School of Biological Sciences, Nara Institute of Science and Technology

• Oshita Yoko

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology

• Hashimoto T

  Graduate School Of Agricultural Sci. Kobe Univ.

• Nakajima Keiji

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology

• Yamada Yuko

  Kyoto Univ. Kyoto Jpn

• Yamaguchi Yuko

  Laboratory For Molecular Functions Of Food Division Of Food Science And Biotechnology Graduate Schoo

• Hashimoto Takashi

  Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol.

• Hashimoto Takashi

  Graduate School Of Biol. Sci.

• Hashimoto Takashi

  Graduate School of Agricultural Science, Kobe University

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