Cloning and Nucleotide Sequence of the AgeI Methylase Gene from Agrobacterium gelatinovorum IAM 12617, a Marine Bacterium

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概要

• 論文の詳細を見る

• The AgeI restriction-modification system from a marine bacterium, Agrobacterium gelatinovorum IAM12617, recognizes the nucleotide sequence ACCGGT. The gene coding for the AgeI methylase (M.AgeI) was cloned into Escherichia coli DH5 αMCR, and the nucleotides of the gene were sequenced. The M.AgeI gene coded for a protein of 429 amino acid residues (molecular mass, 47,358 daltons). The deduced amino acid sequence of M.AgeI was compared with those of other methylases and showed that there are high degrees of similarity in some cytosine-5 methylases.

• 社団法人日本農芸化学会の論文
• 1996-03-23

著者

• Suzuki Tomomi

  School Of Agriculture Bioscience Center Nagoya University

• Yamada Y

  The Department Of Mechanical Engineering And Intelligent Systems University Of Electro-communication

• YAMADA Yuzo

  Laboratory of Applied Microbiology, Department of Applied Biological Chemistry, Faculty of Agricultu

• Yamada Yukio

  Fine Chemicals Research Laboratories Kaneka Corporation

• Yamada Yukio

  Mechanical Engineering Laboratory Material Science And Bioengineering Department Aist

• Suzuki T

  Division Of Marine Life Sciences Grauate School Of Fisheries Science Hokkaido University

• Yamada Y

  Fac. Of Education Utsunomiya Univ.

• TAHARA Yasutaka

  Laboratory of Applied Microbiology, Department of Agricultural Chemistry, Shizuoka University

• TAHARA Yasutaka

  Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University

• Tahara Y

  Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University

• SUZUKI Toshihiko

  United Graduate School of Agricultural Science, Gifu University (Shizuoka University)

• SUGIMOTO Emi

  Laboratory of Applied Microbiology, Department of Applied Biological Chemistry, Faculty of Agricultu

• Sugimoto Emi

  Laboratory Of Applied Microbiology Department Of Applied Biological Chemistry Faculty Of Agriculture

• Yamada Yuzo

  Laboratory Of Applied Microbioligy Department Of Agricultural Chemistry Shizuoka University

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