Cloning and Nucleotide Sequence of ApaLI Restriction-modification System from Acetobacter pasteurianus IFO 13753

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概要

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• The ApaLI restriction-modification system from Acetobacter pasteurianus IFO l3753 recognizes the nucleotide sequence GTGCAC. The gene coding for the ApaLI methylase (M.ApaLI) was cloned into Escherichia coli DH5αMCR, and the nucleotide sequence of the gene was analyzed. The M.ApaLI gene coded for a protein of 429 amino acid residues (molecular mass, 46,554 daltons). The ApaLI restriction endonuclease (R.ApaLI) gene was analyzed by inverse polymerase chain reaction. The R.ApaLI gene coded for a protein of 375 amino acid residues (molecular mass, 42,143 daltons). The two genes had the same orientation separated by two base pairs. The deduced amino acid sequence of M.ApaLI shows significant similarities to the family of cytosine-5 methylases. However, the deduced amino acid sequence of R.ApaLI did not have as much relatedness in the nucleotide sequence, when compared with those of the other restriction endonucleases already reported.

• 社団法人日本農芸化学会の論文
• 1996-09-23

著者

• Suzuki Tomomi

  School Of Agriculture Bioscience Center Nagoya University

• Yamada Y

  The Department Of Mechanical Engineering And Intelligent Systems University Of Electro-communication

• YAMADA Yuzo

  Laboratory of Applied Microbiology, Department of Applied Biological Chemistry, Faculty of Agricultu

• Yamada Yukio

  Fine Chemicals Research Laboratories Kaneka Corporation

• Yamada Yukio

  Mechanical Engineering Laboratory Material Science And Bioengineering Department Aist

• Suzuki T

  Division Of Marine Life Sciences Grauate School Of Fisheries Science Hokkaido University

• Yamada Y

  Fac. Of Education Utsunomiya Univ.

• TAHARA Yasutaka

  Laboratory of Applied Microbiology, Department of Agricultural Chemistry, Shizuoka University

• TAHARA Yasutaka

  Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University

• Tahara Y

  Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University

• SUZUKI Toshihiko

  United Graduate School of Agricultural Science, Gifu University (Shizuoka University)

• SUGIMOTO Emi

  Laboratory of Applied Microbiology, Department of Applied Biological Chemistry, Faculty of Agricultu

• Sugimoto Emi

  Laboratory Of Applied Microbiology Department Of Applied Biological Chemistry Faculty Of Agriculture

• Yamada Yuzo

  Laboratory Of Applied Microbioligy Department Of Agricultural Chemistry Shizuoka University

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