Genomic Structure and Promoter Analysis of Phosphoenolpyruvate Carboxylase in a C3 Plant, Nicotiana sylvestris(Biochemistry & Molecular Biology)

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概要

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• Three genes encoding phosphoenolpyruvate carboxylase were isolated from Nicotiana sylvestris and designated Nsppc1-3, Sequencing of nucleotides showed that the coding sequence and deduced amino acid sequences were highly conserved among the genes, but sequences for noncoding regions including introns and 5'-flanking regions were not conserved. Analysis of the transcript level of the genes by a combination of reverse transcriptase-PCR and restriction fragment polymorphism showed mostNsppc1 in the leaves, stems, roots, and cultured cells of N. sylvestris. β-Glucuronidase activity was detected histochemically in mesophyll cells in leaves, lateral buds, and vascular bundles in roots of transgenic tobacco harboring a chimeric construct of the Nsppc1 promoter and the gene for β-glucuronidase. Deletion analysis indicated the presence of a silencer-like element for basal expression in the promoter region of Nsppc1.

• 社団法人日本農芸化学会の論文
• 2002-08-23

著者

• Sato Fumihiko

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

• Yamada Y

  Doshisha Women's Coll. Liberal Arts Kyoto Jpn

• Yamada Y

  The Department Of Mechanical Engineering And Intelligent Systems University Of Electro-communication

• Sato F

  Div. Of Integrated Life Sci. Graduate School Of Biostudies Kyoto Univ.

• YAMADA Yasuyuki

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

• Yamada Yukio

  Fine Chemicals Research Laboratories Kaneka Corporation

• Yamada Yasuyuki

  Graduate School Of Biological Science Nara Institute Of Science And Technoiogy

• Kitajima Sakihito

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University:(present Address)

• Kitajima Sakihito

  Ri Ctr. Kyoto Univ.

• Kitajima Sakihito

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Yamada Yukio

  Mechanical Engineering Laboratory Material Science And Bioengineering Department Aist

• Sato F

  Division Of Integrated Life Science Graduate School Of Biostudies Kyoto University

• Yamada Y

  Fac. Of Education Utsunomiya Univ.

• Toyota K

  Kyoto Univ. Kyoto Jpn

• Yamada Y

  Faculty Of Education Utsunomiya University

• KOIOZUMI Nozomu

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

• TOYOTA Kentaro

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

• Toyota Kentaro

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Koiozumi Nozomu

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University:research And Educa

• Sato Fumihiko

  Graduate School Of Biostudies Kyoto University

• Yamada Yuko

  Kyoto Univ. Kyoto Jpn

• Sato Fumihiko

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Yamaguchi Yuko

  Laboratory For Molecular Functions Of Food Division Of Food Science And Biotechnology Graduate Schoo

• Koizumi Nozomu

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

• Sato Fumihiko

  Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University

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