Development of a Simple Non-Freezing Method to Preserve Cultured Plant Cells
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概要
- 論文の詳細を見る
A simple procedure to preserve suspended or immobilized plant cell culture (Lavandula vera and Nicotiana tabacum) has been developed. L. vera cells were stored under dark and cool (4℃)conditions in sealed glass bottles. When medium was removed from the system with the immobilized cells, enhanced viability after long-term preservation was obtained. The cells were able to grow successfully after storage for 30 d, and incubation for only 10 d after storage was enough to recover the oxygen uptake activity to the level of that of the original cells. L. vera cells retained the ability to synthesize blue pigments, specific secondary metabolites of the cells, even after storage. Entrapment in calcium alginate beads was effective to preserve the cells more than 50 d. The importance of air supply in preservation was strongly suggested. N. tabacum cells could also be preserved for 30 d.
- 社団法人日本生物工学会の論文
- 1991-08-25
著者
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YAMADA Yasuyuki
Department of Thoracic and Cardiovascular Surgery, Dokkyo Medical University
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TAKAHASHI Junzo
Department of Dental Materials Science and Technology, Faculty of Dentistry, Osaka University
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Sato Fumihiko
Department of Medicine, Metabolism and Endocrinology, Juntendo University School of Medicine
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Sonomoto Kenji
Lab. Of Functional Food Design Dep. Of Functional Metabolic Design Bio-architecture Center Kyushu Un
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Sonomoto Kenji
Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyoto University:(
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Sonomoto Kenji
Laboratory Of Microbial Technology Department Of Food Science And Technology Faculty Of Agriculture
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Sonomoto Kenji
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
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Sato Fumihiko
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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ICHIMURA Kunihiro
Research Institute for Science and Technology, Science University of Tokyo
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Nakajima Hiroki
Department Of Industrial Chemistry Faculty Of Engineering Kyoto University
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Sonomoto Kenji
Laboratory Of Microbial Technology Department Of Bioscience And Biotechnology Faculty Of Agriculture
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Yamada Yukio
Fine Chemicals Research Laboratories Kaneka Corporation
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Tanaka Atsuo
Department Of Applied Physics Faculty Of Engineering Osaka University
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Yamada Yasuyuki
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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Ichimura Kunihiro
Research Institute For Polymers And Textiles
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Ichimura Kunihiro
Research Center For Materials With Integrated Properties Toho University
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Yamada Yukio
Mechanical Engineering Laboratory Material Science And Bioengineering Department Aist
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Tanaka A
Kao Corp. Wakayama Jpn
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Nakajima H
Basic Technology Department Research And Development Center Unitika Ltd.
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Takahashi J
Division Of Oral Maxillofacial Regeneration Graduate School Of Dentistry Osaka University
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SUNOMOTO KENJI
(Present address)Department of Biochemical Engineering and Science, Faculty of Computer Science and
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Takahashi Junzo
The Research Institute For Iron Steel And Other Metals Tohoku University
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Takahashi Junzo
Department Of Dental Materials Science And Technology Faculty Of Dentistry Osaka University
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Takahashi Junzo
Department Of Industrial Chemistry Faculty Of Engineering Kyoto University
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Yamada Yuko
Kyoto Univ. Kyoto Jpn
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Nakajima Hiroki
Department Of Diagnostic Pathology Gunma University Graduate School Of Medicine
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Sato Fumihiko
Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University
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