Characterization of Accumulation of Tobacco PR-5 Proteins by IEF-Immunoblot Analysis : ENVIRONMENTAL AND STRESS RESPONSES : PROTEINS, ENZYMES AND METABOLISM

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概要

• 論文の詳細を見る

• Accumulation of group 5 pathogenesis-related (PR) proteins in tobacco was characterized by immunological analysis combined with isoelectric focusing. This method clearly distinguished 3 subclasses of PR-5 proteins; basic (osmotin), neutral (osmotin-like protein: OLP) and acidic (PR-S). A high accumulation of PR-5 proteins was detected only in root tissues in which neutral osmotin-like protein was mainly accumulated, in addition to a small amount of osmotin. Immunohistochemical analysis revealed that OLP accumulated in the cortex of the root. Cultured tobacco cells accumulated large amounts of neutral PR-5 proteins (OLP) in cells and the acidic form (PR-S) in the medium. Adaptation to salt stress was associated with a higher accumulation of basic PR-5 (osmotin) and less neutral PR-5. In an analysis of the effect of biotic and abiotic stimuli on the synthesis of PR-5 proteins in leaf tissues, ethylene was found to induce a high accumulation of basic and neutral PR-5 proteins. Tobacco mosaic virus (TMV) infection induced accumulation of all major PR-5 proteins, but induction of OLP was less than that of the other isoforms. Systemic induction of PR-5 proteins in upper non-infected leaves was not observed. Salicylate induced only a small accumulation of PR-S. These results indicate that each PR-5 protein has an independent regulatory pathway for its gene expression.

• 日本植物生理学会の論文

著者

• YAMADA Yasuyuki

  Department of Thoracic and Cardiovascular Surgery, Dokkyo Medical University

• Sato Fumihiko

  Department of Medicine, Metabolism and Endocrinology, Juntendo University School of Medicine

• Sato Fumihiko

  Div. Integ. Life Sci. Grad. Sch. Biostudies Kyoto Univ.:plant Function Lab.

• Sato Fumihiko

  Grad. Sch. Agri. Kyoto Univ.:grad. Sch. Biostudies Kyoto Univ.

• Sato Fumihiko

  Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University

• Yamada Yasuyuki

  Grad. School Of Biol. Sci. Naist

• Yamada Yasuyuki

  Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University

• Yamada Yasuyuki

  Graduate School Of Biological Science Nara Institute Of Science And Technoiogy

• Sato Fumihiko

  Laboratory Of Molecular And Cellular Biology Of Totipotency Division Of Integrated Life Science Grad

• Sato F

  Division Of Integrated Life Science Graduate School Of Biostudies Kyoto University

• Yamada Y

  Present Address: Graduate School Of Biological Science Nara Institute Of Science And Technology

• Koiwa Hisashi

  Department Of Applied Life Sciences Kyoto University

• KATO Hiroaki

  RIKEN Harima Inst.

• Yamada Yuko

  Kyoto Univ. Kyoto Jpn

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